Yeast-based self-organized hybrid bio-silica sol–gels for the design of biosensors

Yeast Debaryomyces hansenii within ORMOSIL Shells as a Heterogeneous Biocatalyst

“…The spontaneous formation of similar structures was observed earlier in the case of the methylotrophic yeasts Pichia angusta BKM Y-2559 and the oleaginous yeasts Cryptococcus curvatus VKM Y-3288 [12].…”

“…In that study, we showed the dynamics of the formation of sol particles and fractal gel structure on the surface of yeast cells. Also, the direct participation of yeast in the spontaneous formation of 3D-structure of the cell-in-shell biohybrids was demonstrated [12]. It should be noted that in the process of the sol-gel synthesis, when alkoxysilanes undergo hydrolysis and polycondensation, alcohol is released and can be toxic for living cells.…”

“…Unlike the oleaginous yeasts Cryptococcus curvatus VKM Y-3288, the methylotrophic yeasts Pichia angusta VKM Y-2559 consume alcohols as substrates and retain their activity after encapsulation under these conditions. This allowed the use of their encapsulated form to develop a biosensitive system for methanol detection [12] and to create a biofilter for the recovery of methanol wastewater [13]. At the same time, the acetic acid bacteria Gluconobacter oxydans cannot be encapsulated under the same conditions, which suggests that other microorganisms cannot always form protective shells on the cell surface and save their vitality.…”

“…McElveen buffers (Sigma) with different pH values (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) were placed in cuvettes with subsequent addition of samples.…”

Tuning the Surface Properties of Oxygen-Rich and Nitrogen-Rich Plasma Polymers: Functional Groups and Surface Charge

“…Biomaterials find applications mainly in implants, biosensors, tissue engineering and regenerative medicine [1][2][3][4][5][6][7]. They provide favorable physical and chemical environment that can regulate the behavior and biological functions of microorganisms [2,8].…”