Yeast-Based Biosynthesis of Natural Products From Xylose

Abstract

Xylose is the second most abundant sugar in lignocellulosic hydrolysates. Transformation of xylose into valuable chemicals, such as plant natural products, is a feasible and sustainable route to industrializing biorefinery of biomass materials. Yeast strains, including Saccharomyces cerevisiae, Scheffersomyces stipitis, and Yarrowia lipolytica, display some paramount advantages in expressing heterologous enzymes and pathways from various sources and have been engineered extensively to produce natural products. In this review, we summarize the advances in the development of metabolically engineered yeasts to produce natural products from xylose, including aromatics, terpenoids, and flavonoids. The state-of-the-art metabolic engineering strategies and representative examples are reviewed. Future challenges and perspectives are also discussed on yeast engineering for commercial production of natural products using xylose as feedstocks.

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

“…Yeasts are recognized as promising host microorganisms for chemicals and biofuels production from biomass feedstocks ( Do et al, 2019 ; Yaguchi et al, 2018 ; Zha et al, 2021 ) and many research groups have successfully produced resveratrol from glucose using yeast species such as S. cerevisiae and Y. lipolytica as a host for construction of the recombinant resveratrol producing strains ( Li et al, 2016a , Li et al, 2016b ; Sáez-Sáez et al, 2020 ) . However, there have been no reports on the use of biomass-derived sugars, other than glucose, as carbon sources for resveratrol production, and the effects of the assimilation of these sugars on resveratrol production have not been investigated.…”

Lipid and carotenoid production by Rhodosporodium toruloides ATCC 204091 using C5 and C6 sugars obtained from lignocellulosic hydrolysate

“…Xylose is a five-carbon atom (C5) monosaccharide which is the second most plentiful sugar present in plant biomass (Tiukova et al, 2019). Its consumption by microorganisms is considered as a challenge to attain a sustainable valorization of plant biomass and commercialization of lignocellulosic fuels and chemicals (Nogué and Karhumaa, 2015;Zha et al, 2021). The yeast was able to produce lipid and carotenoids simultaneously when grown in xylose-rich medium of hydrolysate (C5), but the final biomass and lipid production was lesser than that of glucose rich medium (C6).…”

Workflows for optimization of enzyme cascades and whole cell catalysis based on enzyme kinetic characterization and pathway modelling

“…In general, the suitability of a pathway for an application depends on the desired product and therefore each of the D-xylose degradation pathways may be preferable for a specific biotechnological approach [5,11,25] depending on the primary D-xylose degradation product of the pathway and the required precursor for the desired product formation (Figure 2). And even the simultaneous use of two or more of the pathways can have synergistic effects.…”