Yeast as a Biocatalyst in Microbial Fuel Cell

Sayed, Enas Taha; Abdelkareem, Mohammad Ali

doi:10.5772/intechopen.70402

Cited by 22 publications

(18 citation statements)

References 62 publications

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“…In the present paper we emphasized the use of a simple commercial DCFC device, coupled with Saccharomyces Cerevisiae cells, for analytical purpose, i.e., for glucose determination. As reported in the reference [ 17 ] of the present paper, other researchers also addressed the same topic, especially to investigate energy production. However, as can easily be seen, reading the paper cited in reference [ 17 ], practically all these researchers have worked on rather complex systems, typically involving the immobilization of yeast in the anodic compartment of the fuel cell along with an exogenous mediator, like methylene blue, or bromocresol, or eriochrome, and so on.…”

Section: Introductionmentioning

confidence: 88%

“…Enzymatic glucose biofuel cells were fabricated [ 12 , 13 , 14 ], using enzymes such as glucose oxidase and laccase as electrocatalysts, however they have limited stability [ 8 ]. Lastly microbial fuel cells have been studied, where the different systems of a whole electroactive micro-organism were used [ 15 , 16 , 17 ]. Among fuel cells, those based on biological processes are particularly appealing for their inherent sustainability.…”

Section: Introductionmentioning

confidence: 99%

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Simple Yeast-Direct Catalytic Fuel Cell Bio-Device: Analytical Results and Energetic Properties

et al. 2021

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This paper reports the analytical detection and energetic properties of a glucose-fed Direct Catalytic Fuel Cell (DCFC) operated in association with yeast cells (Saccharomyces Cerevisiae). The cell was tested in a potentiostatic mode, and the operating conditions were optimized to maximize the current produced by a given concentration of glucose. Results indicate that the DCFC is characterized by a glucose detection limit of the order to 21 mmol L−1. The cell was used to estimate the “pool” of carbohydrate content in commercial soft drinks. Furthermore, the use of different carbohydrates, such as fructose and sucrose, has been shown to result in a good current yield.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Simple Yeast-Direct Catalytic Fuel Cell Bio-Device: Analytical Results and Energetic Properties

et al. 2021

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“…Carbon and carbon-based materials are commonly used as electrodes for MFC as they are cheap, biocompatible, have a good electrical conductivity, and are available in different morphologies, such as carbon paper (CC), carbon cloth, carbon felt, and carbon brush [41][42][43][44]. The different parameters affecting the MFC performance include everything from the membrane, electrode type and structure, biocatalyst, cell design, pH, and concentration of the anode [45][46][47][48]. In addition, the anode configuration and structure play an important role in cell performance.…”

Section: Introductionmentioning

confidence: 99%

A Carbon-Cloth Anode Electroplated with Iron Nanostructure for Microbial Fuel Cell Operated with Real Wastewater

et al. 2020

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Microbial fuel cell (MFC) is an emerging method for extracting energy from wastewater. The power generated from such systems is low due to the sluggish electron transfer from the inside of the biocatalyst to the anode surface. One strategy for enhancing the electron transfer rate is anode modification. In this study, iron nanostructure was synthesized on a carbon cloth (CC) via a simple electroplating technique, and later investigated as a bio-anode in an MFC operated with real wastewater. The performance of an MFC with a nano-layer of iron was compared to that using bare CC. The results demonstrated that the open-circuit voltage increased from 600 mV in the case of bare CC to 800 mV in the case of the iron modified CC, showing a 33% increase in OCV. This increase in OCV can be credited to the decrease in the anode potential from 0.16 V vs. Ag/AgCl in the case of bare CC, to −0.01 V vs. Ag/AgCl in the case of the modified CC. The power output in the case of the modified electrode was 80 mW/m2—two times that of the MFC using the bare CC. Furthermore, the steady-state current in the case of the iron modified carbon cloth was two times that of the bare CC electrode. The improved performance was correlated to the enhanced electron transfer between the microorganisms and the iron-plated surface, along with the increase of the anode surface- as confirmed from the electrochemical impedance spectroscopy and the surface morphology, respectively.

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“…Concomitantly, laccases catalyze the four-electron reduction of dioxygen to water at a tri-copper site in the interior of the protein without the production of superoxides or peroxides [17]. Despite their promise, laccase cathodes are plagued by short enzyme lifetimes, suboptimal enzymatic reaction velocities, enzyme inactivation, and low enzyme adsorption [6,15,[18][19][20][21][22]. Many attempts have been made to engineer laccases with faster reaction velocities, but additional work is required to address losses from these other factors [23,24].…”

Section: Introductionmentioning

confidence: 99%

A Hybrid Microbial-Enzymatic Fuel Cell Cathode Overcomes Enzyme Inactivation Limits in Biological Fuel Cells

Gervasio¹,

Evans²,

Pryor³

2020

Preprint

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The construction of optimized biological fuel cells requires a cathode which combines the longevity of a microbial catalyst with the power density of an enzymatic catalyst. Laccase secreting fungi were grown directly on the cathode of a biological fuel cell to facilitate the exchange of inactive enzymes with active enzymes with the goal of extending the lifetime of laccase cathodes. Additionally, a functionally graded coating was developed to increase enzyme loading at the cathode. Directly incorporating the laccase producing fungus at the cathode extends the operational lifetime of laccase cathodes while eliminating the need for frequent replenishment of the electrolyte. Additionally, the hybrid microbial-enzymatic cathode addresses the issue of enzyme inactivation by using the natural ability of fungi to exchange inactive laccases at the cathode with active laccases. Finally, enzyme adsorption was increased through the use of a functionally graded coating containing an optimized ratio of titanium dioxide nanoparticles and single walled carbon nanotubes. The hybrid microbial-enzymatic fuel cell combines the higher power density of enzymatic fuel cells with the longevity of microbial fuel cells and demonstrates the feasibility of a self-regenerating fuel cell in which inactive laccases are continuously exchanged with active laccases.

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Yeast as a Biocatalyst in Microbial Fuel Cell

Abstract: Microbial fuel cells (MFCs) are fascinating bioelectrochemical devices that use the catalytic activity of living microorganisms to draw electric energy from organic matter pres-

Cited by 22 publications

References 62 publications

Simple Yeast-Direct Catalytic Fuel Cell Bio-Device: Analytical Results and Energetic Properties

Simple Yeast-Direct Catalytic Fuel Cell Bio-Device: Analytical Results and Energetic Properties

A Carbon-Cloth Anode Electroplated with Iron Nanostructure for Microbial Fuel Cell Operated with Real Wastewater

A Hybrid Microbial-Enzymatic Fuel Cell Cathode Overcomes Enzyme Inactivation Limits in Biological Fuel Cells

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