Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall

Ribeiro, Ricardo A.; Vitorino, Miguel V.; Godinho, Cláudia P.; Bourbon-Melo, Nuno; Robalo, Tiago T.; Fernandes, Fábio; Rodrigues, Mário S.; Sá‐Correia, Isabel

doi:10.1038/s41598-021-92069-3

Cited by 34 publications

(61 citation statements)

References 42 publications

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“…Therefore, in order to avoid false fluorescence signals when using VFAs medium together with Nile Red, it is highly recommended to conduct a throughout wash of the samples. Different culture media can affect inherent features of yeast cells (cell morphology, lipid droplet size or cell wall thickness) [ 27 ]. However, as it can be seen in Fig.…”

Section: Resultsmentioning

confidence: 99%

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Morales-Palomo

Liras

González‐Fernández

et al. 2022

Biotechnol Biofuels

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Background Microbial lipids are found to be an interesting green alternative to expand available oil sources for the chemical industry. Yeasts are considered a promising platform for sustainable lipid production. Remarkably, some oleaginous yeasts have even shown the ability to grow and accumulate lipids using unusual carbon sources derived from organic wastes, such as volatile fatty acids. Recent research efforts have been focused on developing rapid and accurate fluorometric methods for the quantification of intracellular yeast lipids. Nevertheless, the current methods are often tedious and/or exhibit low reproducibility. Results This work evaluated the reliability of different fluorescence measurements (fluorescence intensity, total area and fluorescence quantum yield) using Nile Red as lipid dye in two yeast strains (Yarrowia lipolytica ACA-DC 50109 and Cutaneotrichosporon curvatum NRRL-Y-1511). Different standard curves were obtained for each yeast specie. Fermentation tests were carried with 6-month difference to evaluate the effect of the fluorometer lamp lifetime on lipid quantification. Conclusions Fluorescence quantum yield presented the most consistent measurements along time and the closer estimations when compared with lipids obtained by conventional methods (extraction and gravimetrical determination). The need of using fluorescence quantum yield to estimate intracellular lipids, which is not the common trend in studies focused on microbial lipid production, was stressed. The information here provided will surely enable more accurate results comparison.

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Section: Resultsmentioning

confidence: 99%

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Morales-Palomo

Liras

González‐Fernández

et al. 2022

Biotechnol Biofuels

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“…During formation, a higher substrate concentration results in a higher conversion of carbohydrates in ethanol and other secondary metabolites (organic acids; Dzialo et al ., 2017). Accumulation of alcohol and organic acids induces stress to the yeast due to decreased intracellular pH (Attfield, 1997; Ribeiro et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

Performance of wild Saccharomyces and Non‐Saccharomyces yeasts as starter cultures in dough fermentation and bread making

Condessa

Silva

et al. 2022

Int J of Food Sci Tech

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Summary The use of wild Saccharomyces and non‐Saccharomyces yeasts might result in bread with different and attractive sensory characteristics. This study aimed to evaluate the performance of Saccharomyces and non‐Saccharomyces yeasts as starter culture in dough fermentation to bread making and the physicochemical parameters and aromatic profile of bread. All 26 wild yeasts strains isolated from Brazilian Cerrado fruit and tree bark were osmotolerant, and 19.4% were able to ferment maltose. Candida tropicalis ART101.3 and Saccharomyces cerevisiae SC5952 had the best growth capacity under high concentrations of glucose and maltose. Also, they were resistant to lyophilisation. Kinetic parameters of bioreactor cultivations showed high cell growth and lower generation time with 10 g L−1 maltose. Bread produced with C. tropicalis ART101.3 and the control bread had similar physicochemical properties and acceptance of consumers. Bread with S. cerevisiae SC5951 had a lower specific volume and a different colour than control bread; however, the consumers found no significant difference. More than 70% of the consumers demonstrated purchase intention of bread produced with both wild yeasts. The present study shows the potential of native Cerrado yeasts to be used and exploited in industrial processes and contributes to the diversification of bread starter cultures.

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“…Trehalose metabolism ( TPS2 , TSL1 , and ATH1 ) has been found to protect cell biomacromolecules from stress effects caused by acetic acid, high temperature, and high osmotic pressure [ 86 , 89 ]. Glucan synthesis ( FKS1 , FKS2 , and ROM2 ) has been found to involve in cell wall remodeling under acetic acid stress [ 90 , 91 ]. The other carbohydrates, such as inositol phosphate and galactose, also play indelible roles in strains’ response to stress effects [ 41 ].…”

Section: Response Mechanisms Of S Cerevisiae To St...mentioning

confidence: 99%

Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains

Liu

Zhao

2022

Biotechnol Biofuels

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Bioconversion of lignocellulosic biomass to biofuels such as bioethanol and high value-added products has attracted great interest in recent decades due to the carbon neutral nature of biomass feedstock. However, there are still many key technical difficulties for the industrial application of biomass bioconversion processes. One of the challenges associated with the microorganism Saccharomyces cerevisiae that is usually used for bioethanol production refers to the inhibition of the yeast by various stress factors. These inhibitive effects seriously restrict the growth and fermentation performance of the strains, resulting in reduced bioethanol production efficiency. Therefore, improving the stress response ability of the strains is of great significance for industrial production of bioethanol. In this article, the response mechanisms of S. cerevisiae to various hydrolysate-derived stress factors including organic acids, furan aldehydes, and phenolic compounds have been reviewed. Organic acids mainly stimulate cells to induce intracellular acidification, furan aldehydes mainly break the intracellular redox balance, and phenolic compounds have a greater effect on membrane homeostasis. These damages lead to inadequate intracellular energy supply and dysregulation of transcription and translation processes, and then activate a series of stress responses. The regulation mechanisms of S. cerevisiae in response to these stress factors are discussed with regard to the cell wall/membrane, energy, amino acids, transcriptional and translational, and redox regulation. The reported key target genes and transcription factors that contribute to the improvement of the strain performance are summarized. Furthermore, the genetic engineering strategies of constructing multilevel defense and eliminating stress effects are discussed in order to provide technical strategies for robust strain construction. It is recommended that robust S. cerevisiae can be constructed with the intervention of metabolic regulation based on the specific stress responses. Rational design with multilevel gene control and intensification of key enzymes can provide good strategies for construction of robust strains. Graphical Abstract

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Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall

Cited by 34 publications

References 42 publications

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Performance of wild Saccharomyces and Non‐Saccharomyces yeasts as starter cultures in dough fermentation and bread making

Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains

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