Yarrowia lipolytica reduces the disease incidence of asparagus infected by Fusarium proliferatum by affecting respiratory metabolism and energy status

Hu, Wenjing; Zhang, Xiaoyun; Godana, Esa Abiso; Gu, Xiaohong; Zhao, Lina; Zhang, Hongyin

doi:10.1016/j.biocontrol.2021.104625

Cited by 21 publications

(6 citation statements)

References 54 publications

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“…The α-KGDH is widely distributed in the mitochondria of animals, plant microorganisms, and cultured cells. It is one of the key enzymes regulating the α-oxidative decarboxylation of ketoglutarate to succinyl coenzyme in the TCA cycle [ 30 , 31 ]. The activity of ICDHm and α-KGDH in each sample was detected according to the instructions of the kit (Solarbio, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Nitric Oxide Negatively Regulates the Rapid Formation of Pleurotus ostreatus Primordia by Inhibiting the Mitochondrial aco Gene

Hou

Huang

et al. 2022

JoF

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Nitric oxide (NO) is as a signaling molecule that participates in the regulation of plant development and in a number of physiological processes. However, the function and regulatory pathway of NO in the growth and development of edible mushrooms are still unknown. This study found that NO played a negative role in the transformation of Pleurotus ostreatus from vegetative growth to reproductive growth by the exogenous addition of NO donors and scavengers. Further studies showed that NO can inhibit the gene expression and enzyme activity of aconitase (ACO). Moreover, the overexpression (OE) of mitochondrial aco and RNA interference (RNAi) confirmed that ACO participates in the regulation of the primordia formation rate. The effects of aco OE and RNAi on the tricarboxylic acid (TCA) cycle and energy metabolism were further measured. The results showed that RNAi-aco mutant strains can affect the enzyme activities of isocitrate dehydrogenase of mitochondria (ICDHm) and α-ketoglutarate dehydrogenase (α-KGDH) in the TCA cycle, thereby reducing the production of nicotinamide adenine dinucleotide (NADH) in the TCA cycle, decreasing the contents of adenosine triphosphate (ATP) and hydrogen peroxide (H2O2), and negatively regulating the rapid formation of primordia. In addition, H2O2 was significantly increased during the transformation from vegetative growth to reproductive growth of P. ostreatus. Additionally, the exogenous addition of H2O2 and its scavengers further confirmed the positive regulation by H2O2 in primordia formation. This study shows that during the growth and development of P. ostreatus, NO can inhibit the expression of the mitochondrial aco gene and ACO protein in the TCA cycle, reduce the production of ATP and H2O2 in the respiratory chain, and negatively regulate the rate of primordia formation.

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Section: Methodsmentioning

confidence: 99%

Nitric Oxide Negatively Regulates the Rapid Formation of Pleurotus ostreatus Primordia by Inhibiting the Mitochondrial aco Gene

Hou

Huang

et al. 2022

JoF

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“…The enzyme activities of pyruvate kinase (PK) (Burrell et al, 1994), aconitase (ACO) (Alexander et al, 2021), mitochondrial isocitrate dehydrogenase (ICDHm) (Hu et al, 2021), Na + ‐K + ‐ATPase (Jin et al, 2014) and Ca 2+ ‐Mg 2+ ‐ATPase (Dayanne et al, 2015) were determined as described previously. ATP content and citrate synthase (CS) activity were determined using ATP and CS test kits (Solarbio, Beijing, China), following the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive proteome and lysine acetylome analysis after artificial aging reveals the key acetylated proteins involved in wheat seed oxidative stress response and energy production

Zhang

Shan

et al. 2022

Journal of Food Biochemistry

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Lysine acetylation is a common post‐translational modification of proteins within all organisms. However, quantitative acetylome characterization in wheat seed during aging in storage has not been reported. This study reports the first large‐scale acetylome analysis of wheat seeds after artificial aging treatment, using the quantitative proteomic approach. In total, 11,002 acetylation sites, corresponding to 4262 acetylated proteins were identified, of which 1207 acetylated sites, representing 783 acetylated proteins, were significantly more or less acetylated after artificial aging. Functional analysis demonstrated that the majority of the acetylated proteins are closely involved with cellular and metabolic functions. In particular, key enzymes in the oxidative stress response and energy metabolism were significantly differentially acetylated and appear to be heavily involved in wheat seed aging. The acetylome analysis was verified by quantitative real‐time PCR and enzyme activity determination. Lysine‐acetylation results in a weaker oxidative stress response and lower energy production efficiency, resulting in the apoptosis of wheat seed cells, insufficient energy supply at the germination stage, and consequently, marked loss of seed vigor. Practical applications It is known that the loss of protein function is an important reason for the decrease of seed vigor. Therefore, the change of protein function in the process of wheat seed aging was studied by proteome and lysine acetylome analysis technology. The results showed that the oxidation–reduction imbalance and the decrease of energy production efficiency of seeds were the important reasons for the decrease of their vigor. This will provide a new idea for green and safe storage of grain.

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“…This could be achieved by using an -omics approach (i.e., metabolomics, transcriptomics, proteomics, etc.). Asparagus quality deterioration may occur during transportation and storage because of pathogen infection (i.e., fungi, bacteria) as well. , Globally, there is an increased demand by consumers and fresh produce distributors for more chemically free fruits and vegetables. Review studies are encouraged to summarize environmentally and human-friendly postharvest treatments for preventing asparagus loss caused by pathogen infection during postharvest storage.…”

Section: Postharvest Treatments For Minimizing Physiological Disorder...mentioning

confidence: 99%

Sustainable Postharvest Treatments for Prolonging Asparagus (Asparagus officinalis L.) Shelf Life by Minimizing the Development of Physiological Disorders

Papoutsis

2023

ACS Food Sci. Technol.

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Asparagus (Asparagus officinalis L.) is a non-climacteric vegetable, well-known for its nutritional properties. Asparagus is consumed either fresh, frozen, or processed into canned food. Fresh asparagus has a short shelf life due to its high respiration rates, which results in asparagus quality loss. Asparagus quality loss is also associated with the development of physiological disorders (i.e., stem discoloration, bract opening, and toughening) during postharvest storage. The current study reviews various sustainable postharvest treatments that can be applied to minimize asparagus postharvest losses by controlling the development of physiological disorders. The mechanisms of action of sustainable treatments and the mechanisms involved in physiological disorder development are comprehensively discussed. Packaging, edible coatings, washing with environmentally and human-friendly compounds, storage in a controlled atmosphere, heat treatment, ultrasound treatment, and electrostatic atomized water particle treatments can successfully maintain asparagus quality. Multidisciplinary studies are encouraged to investigate the mechanism of action of sustainable treatments.

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Yarrowia lipolytica reduces the disease incidence of asparagus infected by Fusarium proliferatum by affecting respiratory metabolism and energy status

Cited by 21 publications

References 54 publications

Nitric Oxide Negatively Regulates the Rapid Formation of Pleurotus ostreatus Primordia by Inhibiting the Mitochondrial aco Gene

Nitric Oxide Negatively Regulates the Rapid Formation of Pleurotus ostreatus Primordia by Inhibiting the Mitochondrial aco Gene

Comprehensive proteome and lysine acetylome analysis after artificial aging reveals the key acetylated proteins involved in wheat seed oxidative stress response and energy production

Sustainable Postharvest Treatments for Prolonging Asparagus (Asparagus officinalis L.) Shelf Life by Minimizing the Development of Physiological Disorders

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