YAP1 mediates survival of ALK-rearranged lung cancer cells treated with alectinib via pro-apoptotic protein regulation

Tsuji, Takahiro; Ozasa, Hiroaki; Aoki, Wataru; Aburaya, Shunsuke; Funazo, Tomoko; Furugaki, Koh; Yoshimura, Yasushi; Yamazoe, Masatoshi; Ajimizu, Hitomi; Yasuda, Yuto; Nomizo, Takashi; Yoshida, Hironori; Sakamori, Yuichi; Wake, Hiroaki; Kim, Young Hak; Hirai, Toyohiro

doi:10.1038/s41467-019-13771-5

Cited by 57 publications

(61 citation statements)

References 67 publications

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“…The YAP1/Hippo signaling pathway was closely associated with GC progression, and YAP1induced dysregulation of Hippo pathway contributed to cancer development [36]. According to the previous data, YAP1 served as an oncogene in GC, and targeting YAP1 was effective to restrain cancer progression [37][38][39], which were supported by our data that YAP1 was high-expressed in GC tissues and cells, and GC patients with high-expressed were prone to have a worse prognosis. In addition, recent data hinted that YAP1 could be epigenetically silenced by its upstream miRNAs [48,49], and we surprisingly evidenced that miR-16-5p targeted the 3 UTR of YAP1 mRNA for its inhibition and degradation.…”

Section: Discussionsupporting

confidence: 77%

Long non-coding RNA LINC00649 regulates YES-associated protein 1 (YAP1)/Hippo pathway to accelerate gastric cancer (GC) progression via sequestering miR-16-5p

Wang¹,

Di²,

Bi³

et al. 2021

Bioengineered

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Although long non-coding RNA (LncRNA) LINC00649 is reported to be closely associated with acute myeloid leukemia (AML), prostate cancer and colorectal cancer, its role in regulating other types of cancer, such as gastric cancer (GC), has not been studied. This study analyzed the expression status of LINC00649 in GC tissues and cells by performing Real-Time qPCR analysis, and we found that LINC00649 tended to be enriched in cancerous tissues and cells but not in their normal counterparts, which were supported by the data from TCGA dataset. Next, by performing the gain-and loss-of-function experiments, we expectedly found that LINC00649 acted as an oncogene to accelerate GC cell proliferation, migration and epithelial-mesenchymal transition (EMT) in vitro and promote its tumorigenesis in vivo. Moreover, the online miRDB software predicted that miR-16-5p bound to both LINC00649 and 3 untranslated region (3 UTR) of YAP1 mRNA, which were validated by the following dual-luciferase reporter gene system assay and RNA pull-down assay. Finally, we proved that LINC00649 exerted its tumor-promoting effects in GC by regulating the miR-16-5p/YES-associated protein 1 (YAP1)/Hippo pathway. Mechanistically, knock-down of LINC00649 suppressed YAP1 expressions by releasing miR-16-5p, resulting in the recovery of the Hippo pathway, which suppressed the expression levels of the downstream oncogenes, including EGFR, SOX2 and OCT4, leading to the inhibition of the malignant phenotypes in GC cells. In conclusion, this study, for the first time, evidenced that LINC00649 promoted GC progression by targeting the miR-16-5p/YAP1/Hippo signaling pathway, which provided potential diagnostic and therapeutic indicators for GC treatment for clinical utilization.

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Section: Discussionsupporting

confidence: 77%

Long non-coding RNA LINC00649 regulates YES-associated protein 1 (YAP1)/Hippo pathway to accelerate gastric cancer (GC) progression via sequestering miR-16-5p

Wang¹,

Di²,

Bi³

et al. 2021

Bioengineered

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“…Furthermore, the morphological changes of the McF-7 breast cancer cells cultured in the presence of matrine also revealed its cytotoxic effect on McF-7 cells. apoptosis induction serves a key role in cancer therapy (35). in the present study, Hoechst 33342 staining and flow cytometry revealed that matrine could cause cellular apoptosis in McF-7 cells.…”

Section: Discussionsupporting

confidence: 56%

Matrine exerts anti‑breast cancer activity by mediating apoptosis and protective autophagy via the AKT/mTOR pathway in MCF‑7 cells

Jinwen

Song

et al. 2020

Mol Med Rep

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Matrine, a major alkaloid isolated from the traditional chinese herb Sophora flavescens, has been used clinically to treat breast cancer in china. However, the effects of matrine on apoptosis and autophagy in breast cancer cells remain unclear. in the present study, the anti-breast cancer capacity of matrine was evaluated and its role in regulating apoptosis and autophagy in vitro was investigated. Matrine significantly inhibited the growth of McF-7 cells. in addition, Hoechst 33342 staining and annexin V/propidium iodide staining demonstrated that incubation with matrine induced apoptosis in McF-7 cells. Furthermore, matrine induced autophagy in McF-7 cells, manifesting as an accumulation of light chain 3 ii and downregulation of p62. additionally, matrine suppressed aKT and mammalian target of rapamycin (mTor) phosphorylation, indicating that the aKT/mTor pathway is involved in matrine-induced apoptosis and autophagy. overall, the results of the present study indicated that matrine possesses anti-breast cancer activity by providing protective autophagy via inhibition of the aKT/mTor pathway. These findings indicated that matrine may be a promising candidate for drug development targeting breast cancer.

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“…The proteins in the sample were subjected to tandem mass tag-labeling proteomic analysis, according to previously described methods ( Tsuji et al, 2020 ). Proteins were identified by MASCOT search against the S. vesiculosa HM13 protein database.…”

Section: Methodsmentioning

confidence: 99%

Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13

et al. 2021

Self Cite

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Bacteria secrete and utilize nanoparticles, called extracellular membrane vesicles (EMVs), for survival in their growing environments. Therefore, the amount and components of EMVs should be tuned in response to the environment. However, how bacteria regulate vesiculation in response to the extracellular environment remains largely unknown. In this study, we identified a putative sensor protein, HM1275, involved in the induction of vesicle production at high lysine concentration in a hypervesiculating Gram-negative bacterium, Shewanella vesiculosa HM13. This protein was predicted to possess typical sensing and signaling domains of sensor proteins, such as methyl-accepting chemotaxis proteins. Comparison of vesicle production between the hm1275-disrupted mutant and the parent strain revealed that HM1275 is involved in lysine-induced hypervesiculation. Moreover, HM1275 has sequence similarity to a biofilm dispersion protein, BdlA, of Pseudomonas aeruginosa PAO1, and hm1275 disruption increased the amount of biofilm. Thus, this study showed that the induction of vesicle production and suppression of biofilm formation in response to lysine concentration are under the control of the same putative sensor protein.

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YAP1 mediates survival of ALK-rearranged lung cancer cells treated with alectinib via pro-apoptotic protein regulation

Cited by 57 publications

References 67 publications

Long non-coding RNA LINC00649 regulates YES-associated protein 1 (YAP1)/Hippo pathway to accelerate gastric cancer (GC) progression via sequestering miR-16-5p

Long non-coding RNA LINC00649 regulates YES-associated protein 1 (YAP1)/Hippo pathway to accelerate gastric cancer (GC) progression via sequestering miR-16-5p

Matrine exerts anti‑breast cancer activity by mediating apoptosis and protective autophagy via the AKT/mTOR pathway in MCF‑7 cells

Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13

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