Yap/Taz-TEAD ACTIVITY LINKS MECHANICAL CUES TO CELL PROGENITOR BEHAVIOR DURING HINDBRAIN SEGMENTATION

Voltes, Adria; Cf, Hevia; Dingare, Chaitanya; Calzolari, Simone; Terriente, Javier; Norden, Caren; Lecaudey, Virginie; Pujades, Cristina

doi:10.1101/366351

Cited by 1 publication

(1 citation statement)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies in chick argue against this idea as hindbrain boundaries have a lower proliferation rate than segment centres Peretz et al, 2016), reflecting their role as a pool of neurogenic stem cells. However, recent work has found two-fold greater proliferation at boundaries than segment centres at late stages in the zebrafish hindbrain (after 26 hpf), which depends upon actomyosin and Yap/Taz/Tead activity (Voltes et al, 2018). Since this study only analysed late stages, it did not detect the role in boundary cell specification, which we find occurs prior to 18 hpf.…”

Section: Regulation Of Cell Identity By Taz Activitycontrasting

confidence: 77%

Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness

Mas

Addison

et al. 2019

Preprint

View full text Add to dashboard Cite

The segregation of cells with distinct regional identity underlies formation of a sharp border, which in some tissues serves to organise a boundary signaling centre.It is unclear whether or how border sharpness is coordinated with induction of boundary-specific gene expression. We show that forward signaling of EphA4 is required for border sharpening and induction of boundary cells in the zebrafish hindbrain, which we find both require kinase-dependent signaling, with a lesser input of PDZ domain-dependent signaling. We find that boundary-specific gene expression is regulated by myosin II phosphorylation, which increases actomyosin contraction downstream of EphA4 signaling. Myosin phosphorylation leads to nuclear translocation of Taz, which together with Tead1a is required for boundary marker expression. Since actomyosin contraction maintains sharp borders, there is direct coupling of border sharpness to boundary cell induction that ensures correct organisation of signaling centres.

show abstract

Section: Regulation Of Cell Identity By Taz Activitycontrasting

confidence: 77%