Yanghe Decoction Suppresses the Experimental Autoimmune Thyroiditis in Rats by Improving NLRP3 Inflammasome and Immune Dysregulation

Ma, Binge; Chen, Dexuan; Liu, Yangjing; Zhao, Zhengping; Wang, Jianhua; Zhou, Guowei; Xu, Kun; Zhu, Taiyang; Wang, Qiong; Ma, Chaoqun

doi:10.3389/fphar.2021.645354

Cited by 25 publications

(11 citation statements)

References 47 publications

(53 reference statements)

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“…Therefore, 10 g crude herbs/kg is almost equivalent to the human therapeutic dose, 5 g crude herbs/kg and 20 g crude herbs/kg are almost 0.5-fold and 2-fold of the human therapeutic dose, respectively. The doses of MGQD extract used in mice in this study were also consistent with that in most previous studies, that is, the dosage of decoction in rodents reached crude drug g/kg/d (Luo et al, 2019;Ma et al, 2021;Song et al, 2021). However, the standardization of bioactive compounds in MGQD and future studies at lower doses levels to evaluate the anti-UC potential of MGQD decoction are worthy of further exploration.…”

Section: Discussionsupporting

confidence: 90%

Modified Gegen Qinlian Decoction Regulates Treg/Th17 Balance to Ameliorate DSS-Induced Acute Experimental Colitis in Mice by Altering the Gut Microbiota

Wang

Zhang

et al. 2021

Front. Pharmacol.

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Inflammatory bowel disease (IBD) is characterized by chronic pathology associated with extensive intestinal microbial dysregulation and intestinal inflammation. Thus, efforts are underway to manipulate the gut microbiome to improve inflammatory pathology. Gegen Qinlian decoction (GQD), a traditional Chinese medicine prescription, has been widely utilized for treating diarrhea and ulcerative colitis (UC) for thousands of years. However, the underlying mechanism of its efficacy and whether its protective effect against colitis is mediated by the gut microbiota are poorly understood. In the present study, our data demonstrated that modified GQD (MGQD) administration significantly improved the pathological phenotypes and colonic inflammation challenged by DSS in mice, which were specifically manifested as reduced loss of body weight, shortening of colon length, DAI score, histological score and suppressed inflammatory response. 16S rRNA sequencing and targeted metabonomics analysis showed that MQGD altered the diversity and community landscape of the intestinal microbiota and the metabolic profiles. In particular, MQGD significantly boosted the abundance of the intestinal microbiota producing short-chain fatty acids (SCFAs), which are causally associated with promoting the development of Treg cells and suppressing the differentiation of pro-inflammatory Th17 cells. More importantly, transferring fecal microbiota from MGQD-treated or healthy controls exhibited equivalent alleviative effects on colitis mice. However, this protective effect could not be replicated in experiments of mice with depleted intestinal microbes through broad-spectrum antibiotic cocktails (ABX), further supporting the importance of SCFA-producing gut microbiota in the beneficial role of MGQD. In general, MGQD therapy has the potential to remodel the intestinal microbiome and reestablish immune homeostasis to ameliorate DSS-induced colitis.

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Section: Discussionsupporting

confidence: 90%

Modified Gegen Qinlian Decoction Regulates Treg/Th17 Balance to Ameliorate DSS-Induced Acute Experimental Colitis in Mice by Altering the Gut Microbiota

Wang

Zhang

et al. 2021

Front. Pharmacol.

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“…The main components of Yanghe Decoction include Rehmannia glutinosa, Colla Cornus Cervi, Cinnamomum cassia, Ephedra sinica, Semen Sinapis, Rhizoma Zingiberis Preparata, and Glycyrrhiza. Yanghe Decoction has been proved to play a significant antiinflammatory role in disease treatment through the NLRP3 inflammatory inflammasome [ 11 ], as well as JAK/STAT [ 12 ] and Wnt/ β -catenin [ 13 ] pathways. It is worth noting that Yanghe Decoction has great potential therapeutic value in asthma treatment.…”

Section: Introductionmentioning

confidence: 99%

Yanghe Decoction Effectively Alleviates Lung Injury and Immune Disorder in Asthmatic Mice Induced by Ovalbumin

Tian

Jiang

et al. 2022

Computational Intelligence and Neuroscience

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Objective. To probe into the ameliorative effect of Yanghe Decoction on pulmonary injury and immunologic derangement in asthmatic mice. Methods. C57BL/6 mice were randomized into control (Con), Model, and Yanghe Decoction (YHF) groups, with 12 in each. The asthma model of adult female mice was induced by ovalbumin in the Model group, and the YHF group was treated by Yanghe Decoction on the basis of asthma modeling. The Con group received the same amount of normal saline. Inspiratory resistance (Ri), expiratory resistance (Re), lung compliance (CL), and maximal voluntary ventilation (MVV) were measured after modeling. Lung tissue was collected for the measurement of interleukin (IL)-4, IL-5, IL-6, IL-10, IL-13, and tumor necrosis factor-α (TNF-α) by ELISA kits. Combined with HE staining and PAS staining, the pathological alterations of the lung in each group were observed, and CD4+, Th2, and Th1 contents were determined by flow cytometry (FCM). Results. The pulmonary function (PF) test revealed notably reduced Ri and Re as well as enhanced CL and MVV in asthmatic mice after the application of Yanghe Decoction. Yanghe Decoction dramatically ameliorated the pathological changes of lung tissue in asthmatic mice, as demonstrated by the staining results. ELISA results showed that Yanghe Decoction validly reduces lung tissue IL-4, IL-5, IL-6, IL-13, TNF-α and upregulates IL-10 in asthmatic mice. FCM indicated that Yanghe Decoction obviously reduced the number of Th1 and Th2 cells in asthmatic mice, although it caused the decrease of CD4+ cells, but the difference was not statistically significant. Conclusions. Yanghe Decoction can effectively ameliorate the inflammatory reaction, immune cell disorder, and PF injury in ovalbumin-induced asthmatic mice.

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“…The extraction rate of 21.261% was obtained by evaporation of the concentrate at 100 °C. These doses used in this study were within the range of in vivo studies reported by others (Luo et al, 2019;Lin et al, 2020;Ma et al, 2021;Song et al, 2021).…”

Section: Introductionsupporting

confidence: 61%

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Yanghe Decoction Suppresses the Experimental Autoimmune Thyroiditis in Rats by Improving NLRP3 Inflammasome and Immune Dysregulation

Cited by 25 publications

References 47 publications

Modified Gegen Qinlian Decoction Regulates Treg/Th17 Balance to Ameliorate DSS-Induced Acute Experimental Colitis in Mice by Altering the Gut Microbiota

Modified Gegen Qinlian Decoction Regulates Treg/Th17 Balance to Ameliorate DSS-Induced Acute Experimental Colitis in Mice by Altering the Gut Microbiota

Yanghe Decoction Effectively Alleviates Lung Injury and Immune Disorder in Asthmatic Mice Induced by Ovalbumin

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