Yak miR‐2285o‐3p attenuates hypoxia‐induced apoptosis by targeting <i>caspase‐3</i>

Zhang, J.; Long, Keren; Wang, J.; Jin, Long; Tang, Qianzi; Li, X.; Ma, Jian; Li, M.; Jiang, An An

doi:10.1111/age.13153

Cited by 4 publications

(4 citation statements)

References 26 publications

(49 reference statements)

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“…This gene encodes a member of the DNAJ/HSP40 family of proteins and shows increased expression in peripheral blood from Holstein calves exposed to heat stress [ 79 ]. Similarly, HIGD1A (BTA22 CSS cluster) and CBFA2T3 (a BTA18 CSS cluster distinct from that containing DNAJA2 ) encode proteins associated with cellular responses to hypoxia [ 80 , 81 ], which could be indicative of adaptation to oxidative stress caused by high temperatures [ 82 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic insights into the population history and adaptive traits of Latin American Criollo cattle

Ward,

Ng'ang'a,

Randhawa

et al. 2024

R. Soc. Open Sci.

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Criollo cattle, the descendants of animals brought by Iberian colonists to the Americas, have been the subject of natural and human-mediated selection in novel tropical agroecological zones for centuries. Consequently, these breeds have evolved distinct characteristics such as resistance to diseases and exceptional heat tolerance. In addition to European taurine ( Bos taurus ) ancestry, it has been proposed that gene flow from African taurine and Asian indicine ( Bos indicus ) cattle has shaped the ancestry of Criollo cattle. In this study, we analysed Criollo breeds from Colombia and Venezuela using whole-genome sequencing (WGS) and single-nucleotide polymorphism (SNP) array data to examine population structure and admixture at high resolution. Analysis of genetic structure and ancestry components provided evidence for African taurine and Asian indicine admixture in Criollo cattle. In addition, using WGS data, we detected selection signatures associated with a myriad of adaptive traits, revealing genes linked to thermotolerance, reproduction, fertility, immunity and distinct coat and skin coloration traits. This study underscores the remarkable adaptability of Criollo cattle and highlights the genetic richness and potential of these breeds in the face of climate change, habitat flux and disease challenges. Further research is warranted to leverage these findings for more effective and sustainable cattle breeding programmes.

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Section: Discussionmentioning

confidence: 99%

Genomic insights into the population history and adaptive traits of Latin American Criollo cattle

Ward,

Ng'ang'a,

Randhawa

et al. 2024

R. Soc. Open Sci.

View full text Add to dashboard Cite

show abstract

“…This gene encodes a member of the DNAJ/HSP40 family of proteins and shows increased expression in peripheral blood from Holstein calves exposed to heat stress [95]. Similarly, HIGD1A (BTA22 CSS cluster) and CBFA2T3 (a BTA18 CSS cluster distinct from that containing DNAJA2), encode proteins associated with cellular responses to hypoxia [96][97][98], which could be indicative of adaptation to oxidative stress caused by high temperatures [99].…”

Section: Selection For Heat Tolerance In Criollo Cattlementioning

confidence: 99%

Genomic Insights into the Population History and Adaptive Traits of Latin American Criollo Cattle

Ward,

Ng’ang’a,

Randhawa

et al. 2023

Preprint

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Criollo cattle, descendants of animals brought by Iberian colonists to the Americas, have been subject to centuries of natural and human-mediated selection in novel tropical agroecological zones. Consequently, these breeds have evolved distinct characteristics such as resistance to disease and exceptional heat tolerance. In addition to European taurine (Bos taurus) ancestry, it has been proposed that gene flow from African taurine and Asian zebu (Bos indicus) cattle has shaped the ancestry of Criollo cattle. In this study, we analysed Criollo breeds from Colombia and Venezuela using whole genome sequencing data to examine population structure and admixture at very high resolution. We did not detect evidence of African taurine admixture in Criollo cattle; however, there was evidence of longstanding zebu ancestry in one breed (Hartón del Valle). In addition, we detected selection signatures associated with a myriad of adaptive traits, revealing genes linked to thermotolerance, reproduction, fertility, immunity, and distinct coat and skin coloration traits. This study underscores the remarkable adaptability of Criollo cattle and highlights the genetic richness and potential of these breeds in the face of climate change, habitat flux, and disease challenges. Further research is warranted to leverage these findings for more effective and sustainable cattle breeding programmes.

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“…At the same time, the miR‐2285 family is also a highly expressed bovine‐specific miRNA (Muñoz‐Salinas et al., 2022). miR‐2258o‐3p negatively regulates the target gene phosphatase (phosphatase and tension homologue) and very low‐density lipoprotein receptor (VLDLR) receptor can relieve apoptosis induced by hypoxia (Zhang et al., 2022). There are few reports on the effects of miR‐2285f on milk fat metabolism.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐2285f regulates milk fat metabolism by targeting MAP2K2 in bovine mammary epithelial cells

Zhang,

Wang,

Chen

et al. 2024

Reprod Domestic Animals

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In this study, Holstein dairy cows raised in Ningxia were selected as the research object. Mammary epithelial cells (BMECs) were extracted from the milk of eight Holstein cows with significantly different milk fat expression rates and transcribed for sequencing. Bioinformatics analysis was used to analyse the correlation of fat milk percentage, and the critical miR‐2285f regulating milk fat was screened out. The target gene binding sites were predicted, and 293T cells and mammary epithelial cells were used as miRNA and target gene models for functional verification in vitro. The tissue difference of miR‐2285f Holstein cows was quantitatively analysed by transfecting miR‐2285f mimic and inhibitor. Assay (dual luciferase reporter gene assay) and quantitative real‐time PCR (quantitative real‐time PCR, qRT‐PCR), triglyceride (TAG) detection, oil red O detection of lipid droplets, Western Blot assay, Edu and Flow cytometry, The molecular regulatory effects of miR‐2285f and target gene MAP2K2 on milk fat metabolism of Holstein dairy cows were studied. The wild‐type vector and mutant vector of map2k2‐3′utr were constructed, and double luciferase reporting experiments were conducted to verify that MAP2K2 was one of the target genes of miR‐2285f. According to qRT‐PCR and Western Blot analysis, miR‐2285f mainly regulates the expression of MAP2K2 protein in BMECs at the translation level. Bta‐miR‐2285f can promote cell proliferation and slow cell apoptosis by regulating MAP2K2. Bta‐miR‐2285f can promote triglyceride (TAG) and lipid droplet accumulation in mammary epithelial cells by targeting MAP2K2. Bta‐miR‐2285f can regulate protein levels of fat milk marker gene PPARG by targeting MAP2K2. In conclusion, miR‐2285f can target the expression of the MAP2K2 gene, promote the proliferation of dairy mammary epithelial cells, inhibit cell apoptosis and regulate the milk fat metabolism in dairy mammary epithelial cells. The results of this study revealed the function of miR‐2285f in regulating the differential expression of fat milk in Holstein dairy cows at the cellular level. They provided a theoretical and experimental basis for analysing the regulation network of milk fat synthesis of Holstein dairy cows and the molecular breeding of dairy cows.

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Yak miR‐2285o‐3p attenuates hypoxia‐induced apoptosis by targeting caspase‐3

Cited by 4 publications

References 26 publications

Genomic insights into the population history and adaptive traits of Latin American Criollo cattle

Genomic insights into the population history and adaptive traits of Latin American Criollo cattle

Genomic Insights into the Population History and Adaptive Traits of Latin American Criollo Cattle

MicroRNA‐2285f regulates milk fat metabolism by targeting MAP2K2 in bovine mammary epithelial cells

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