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Crockett, Elahé T; Spielman, William S.; Dowlatshahi, Shadi; He, Jun

doi:10.1186/1476-9255-3-16

Cited by 21 publications

(9 citation statements)

References 36 publications

(54 reference statements)

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“…We used only male C57BL/6 N mice for this primary study because gender of mice affects hormones and cell-mediated immune response [ 20 , 21 ]. Cytokine expression also differs between male and female mice [ 22 ]. Further studies with female mice will be necessary.…”

Section: Methodsmentioning

confidence: 99%

A new multiple trauma model of the mouse

Fitschen-Oestern

Lippross

Klueter

et al. 2017

BMC Musculoskelet Disord

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BackgroundBlunt trauma is the most frequent mechanism of injury in multiple trauma, commonly resulting from road traffic collisions or falls. Two of the most frequent injuries in patients with multiple trauma are chest trauma and extremity fracture. Several trauma mouse models combine chest trauma and head injury, but no trauma mouse model to date includes the combination of long bone fractures and chest trauma. Outcome is essentially determined by the combination of these injuries. In this study, we attempted to establish a reproducible novel multiple trauma model in mice that combines blunt trauma, major injuries and simple practicability.MethodsNinety-six male C57BL/6 N mice (n = 8/group) were subjected to trauma for isolated femur fracture and a combination of femur fracture and chest injury. Serum samples of mice were obtained by heart puncture at defined time points of 0 h (hour), 6 h, 12 h, 24 h, 3 d (days), and 7 d.ResultsA tendency toward reduced weight and temperature was observed at 24 h after chest trauma and femur fracture. Blood analyses revealed a decrease in hemoglobin during the first 24 h after trauma. Some animals were killed by heart puncture immediately after chest contusion; these animals showed the most severe lung contusion and hemorrhage. The extent of structural lung injury varied in different mice but was evident in all animals. Representative H&E-stained (Haematoxylin and Eosin-stained) paraffin lung sections of mice with multiple trauma revealed hemorrhage and an inflammatory immune response. Plasma samples of mice with chest trauma and femur fracture showed an up-regulation of IL-1β (Interleukin-1β), IL-6, IL-10, IL-12p70 and TNF-α (Tumor necrosis factor- α) compared with the control group. Mice with femur fracture and chest trauma showed a significant up-regulation of IL-6 compared to group with isolated femur fracture.ConclusionsThe multiple trauma mouse model comprising chest trauma and femur fracture enables many analogies to clinical cases of multiple trauma in humans and demonstrates associated characteristic clinical and pathophysiological changes. This model is easy to perform, is economical and can be used for further research examining specific immunological questions.

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Section: Methodsmentioning

confidence: 99%

A new multiple trauma model of the mouse

Fitschen-Oestern

Lippross

Klueter

et al. 2017

BMC Musculoskelet Disord

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“…Furthermore, we observed greater dysbiosis of the transcriptome in ApoE4-KI males. There are several studies that have demonstrated evidence of sex differences in the expression of DPGs and inflammation genes 67 – 69 . For example, a 2011 study showed that the release of growth hormone was associated with the difference in the expression of sulfotransferases between sexes 70 .…”

Section: Discussionmentioning

confidence: 99%

Cadmium exposure modulates the gut-liver axis in an Alzheimer’s disease mouse model

et al. 2021

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The human Apolipoprotein E4 (ApoE4) variant is the strongest known genetic risk factor for Alzheimer’s disease (AD). Cadmium (Cd) has been shown to impair learning and memory at a greater extent in humanized ApoE4 knock-in (ApoE4-KI) mice as compared to ApoE3 (common allele)-KI mice. Here, we determined how cadmium interacts with ApoE4 gene variants to modify the gut-liver axis. Large intestinal content bacterial 16S rDNA sequencing, serum lipid metabolomics, and hepatic transcriptomics were analyzed in ApoE3- and ApoE4-KI mice orally exposed to vehicle, a low dose, or a high dose of Cd in drinking water. ApoE4-KI males had the most prominent changes in their gut microbiota, as well as a predicted down-regulation of many essential microbial pathways involved in nutrient and energy homeostasis. In the host liver, cadmium-exposed ApoE4-KI males had the most differentially regulated pathways; specifically, there was enrichment in several pathways involved in platelet activation and drug metabolism. In conclusion, Cd exposure profoundly modified the gut-liver axis in the most susceptible mouse strain to neurological damage namely the ApoE4-KI males, evidenced by an increase in microbial AD biomarkers, reduction in energy supply-related pathways in gut and blood, and an increase in hepatic pathways involved in inflammation and xenobiotic biotransformation.

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“…Sex-based differences in IR have been noted by others in patients with cardiac, renal, and liver injury [ 26 – 30 ]. Our study showed sex-based differences in the clinical phenotype of IR injury (PGD), as well as IR-induced cytokine production, after lung transplantation.…”

Section: Discussionmentioning

confidence: 90%

Analysis of sex-based differences in clinical and molecular responses to ischemia reperfusion after lung transplantation

Chacon‐Alberty¹,

Daoud

et al. 2021

Respir Res

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Background Sex and hormones influence immune responses to ischemia reperfusion (IR) and could, therefore, cause sex-related differences in lung transplantation (LTx) outcomes. We compared men’s and women’s clinical and molecular responses to post-LTx IR. Methods In 203 LTx patients, we used the 2016 International Society for Heart and Lung Transplantation guidelines to score primary graft dysfunction (PGD). In a subgroup of 40 patients with blood samples collected before LTx (T0) and 6, 24, 48 (T48), and 72 h (T72) after lung reperfusion, molecular response to IR was examined through serial analysis of circulating cytokine expression. Results After adjustment, women had less grade 3 PGD than men at T48, but not at T72. PGD grade decreased from T0 to T72 more often in women than men. The evolution of PGD (the difference in mean PGD between T72 and T0) was greater in men. However, the evolution of IL-2, IL-7, IL-17a, and basic fibroblast growth factor levels was more often sustained throughout the 72 h in women. In the full cohort, we noted no sex differences in secondary clinical outcomes, but women had significantly lower peak lactate levels than men across the 72 h. Conclusions Men and women differ in the evolution of PGD and cytokine secretion after LTx: Women have a more sustained proinflammatory response than men despite a greater reduction in PGD over time. This interaction between cytokine and PGD responses warrants investigation. Additionally, there may be important sex-related differences that could be used to tailor treatment during or after transplantation.

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Untitled

Cited by 21 publications

References 36 publications

A new multiple trauma model of the mouse

A new multiple trauma model of the mouse

Cadmium exposure modulates the gut-liver axis in an Alzheimer’s disease mouse model

Analysis of sex-based differences in clinical and molecular responses to ischemia reperfusion after lung transplantation

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