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Abstract

Background: Brucellosis is one of the most common zoonotic infections globally. The laboratory confirmation of human Brucellosis is based on microbiological, serological or molecular methods, each having its own advantages and disadvantages. Aim: To study Brucellosis among patients with pyrexia of unknown origin. Materials and Methods: Blood samples from 111 patients with PUO were taken and blood from 50 healthy controls were also collected. All the samples were tested by IgG and IgM ELISA and Standard Agglutination Test (SAT). IgG ELISA results were compared with SAT for Brucellosis. Results: Anti Brucella IgM antibodies were found in 2 (1.80%) and IgG in 4 (3.60%). 3 patients (2.7%) out of 111 patients were positive by SAT.Sensitivity and specificity of IgG and SAT were 100% and 99.07% respectively. PPV (positive predictive value) and NPV (negative predictive value) were 95% and 100% respectively and diagnostic accuracy was 99.09%.Conclusion:Standard Agglutination Test being cumbersome, time consuming and showing false negativity due to blocking antibodies, IgG ELISA and IgM ELISA can be used for diagnosis of Brucellosis.