Abstract:A xylanase DNA sequence with a total length of 642 bp was previously isolated from a xylanolytic Klebsiella pneumoniae. Xylanase gene primers were designed with the addition of BamH1 and EcoR1 restriction enzyme sites in order get a full xylanase gene that is in-frame with pSTAG expression vector. The isolated xylanase gene was amplified using the designed primers through PCR, then cloned and expressed in E. coli BL21 (DE3). In-silico characterization showed that the recombinant xylanase has a molecular weight… Show more
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