Untitled

Abstract

Background: Blood serum and/or plasma is one of important biomarkers in laboratories to estimate the vital components of blood and is used to determine the healthy status of human and also to diagnose and treat diseases through laboratory results obtained through analysis of serum samples. Wet cupping therapy (WCT), also known as (Al-Hijamah) is one of the therapeutic techniques that were practiced in many countries of the world in ancient times and it is still used in many Islamic countries as considered as one of the beneficial treatment methods for many diseases. Objective: The goal of this study is to identify the effect of storage time and temperature on some biochemical parameters of venous blood serum and compare these results with samples taken from the blood serum of wet cupping therapy, which was treated with the same conditions. Method: Eighteen male participants were selected randomly between ages 25-61 years. About 5 ml of blood samples were collected from the vein and cupping site for each participant and eight biochemical parameters that are glucose, urea, uric acid, creatinine, total cholesterol, triglycerides (TG), high density lipids (HDL), and low density lipids (LDL) were analyzed. Results: All the parameters that were studied gave different results when they were studied at different storage periods i.e. zero time, 24 h, 48 h, and 72 h and at different temperatures (room temperature (26±1 °C) and 2±1 °C). Glucose and high density lipids showed a decrease in concentration with the increase in storage time, while total cholesterol, triglycerides, low density lipids. and uric acid increased their concentration with storage time. Urea and creatinine did not change during the storage period. Conclusion:We recommend avoiding storing the serum for a long period and analyzing it at tested temperatures as soon as possible.