Y5 neuropeptide Y receptor overexpression in mice neither affects anxiety- and depression-like behaviours nor seizures but confers moderate hyperactivity

Neuropeptide Y administration reverses tricyclic antidepressant treatment-resistant depression induced by ACTH in mice

“…Supporting this data, Dos Santos et al (2013) and Jiménez-Vasquez et al (2007) showed antidepressive-like effects with NPY treatment and the overexpression of NPY receptors in mice, respectively. Moreover, we also suggested that this effect can be modulated by Y1, Y2, Y4 and Y5 receptors (Rotzinger et al, 2010;Olesen et al, 2012;Kormos and Gaszner, 2013). It is important to emphasize that the treatment did not cause a sedative effect or excitement as shown in the OFT and lack of change in home-cage food consumption in the NSFT.…”

Anxiolytic-like effects after vector-mediated overexpression of neuropeptide Y in the amygdala and hippocampus of mice

“…Likewise, no effects on immobility were observed after overexpression of Y1 or Y5 receptors (Olesen et al, 2012a(Olesen et al, , 2012b. It remains to be seen whether rAAV-mediated overexpression of NPY transgenes could be efficient in rodents that express depression-like behaviour, e.g.…”

“…The forced swim test (Olesen et al, 2012b) was a slightly modified version from that developed by Porsolt et al (1977). Briefly, mice were placed in glass cylinders (10 × 25 cm) containing 10 cm of water (maintained at 25°C ± 0.5°C).…”

“…Thus, in the amygdala, anxiolytic-like effects have been shown following Herpes simplex (Primeaux et al, 2005) and Sindbis (Thorsell et al, 2007) viral vector-induced NPY overexpression in rats. Mouse studies have shown that recombinant adeno-associated viral (rAAV) vector-induced overexpression of NPY or Y1 receptors, but not Y5 receptors, also confers anxiolytic-like effect in the hippocampus (Lin et al, 2010;Olesen et al, 2012aOlesen et al, , 2012b. Robust reciprocal connections have been shown between the amygdala and hippocampus in the rat (Pitkänen et al, 2000).…”

Exploring the Behavioral and Metabolic Phenotype Generated by Re-Introduction of the Ghrelin Receptor in the Ventral Tegmental Area

“…The transgene was sub-cloned into an rAAV expression cassette, which consisted of the rat neuron-specific enolase promoter, the woodchuck post-transcriptional regulatory element and a bovine growth hormone polyA signal flanked by rAAV inverted terminal repeats. The injections were performed as previously described [70,71]. Briefly, the mice were pre-treated with a mixture of the analgesics Rimadyl (5 mg/kg, Pfizer, New York City, NY, USA) and Temgesic (0.06 mg/kg, Reckit Benckiser, Berkshire, UK) and the antibiotic Baytril (10 mg/kg, Bayer Healthcare, Leverkusen, Germany) before they were anaesthetized using isoflurane gas anesthesia (Baxter, San Juan, Puerto Rico).…”