Y‐STRs in Forensic Medicine: DNA Analysis in Semen Samples of Azoospermic Individuals

Soares-Vieira, José Arnaldo; Billerbeck, Ana Elisa C.; Iwamura, Edna Sadayo Miazato; Zampieri, Ricardo Andrade; Gattás, Gilka Jorge Fígaro; Muñoz, Daniel Romero; Hallak, Jaime Eduardo Cecílio; Mendonca, Berenice B.; Lucon, Antônio Marmo

doi:10.1111/j.1556-4029.2007.00433.x

Cited by 20 publications

(12 citation statements)

References 22 publications

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“…This is consistent with the use of the 12-loci PowerPlex TM Y-System in other studies following detection of seminal markers as PSA and semenogelin [5]. In this sense, multiplexed Y-STR profiles have been obtained in the absence of spermatozoa using 3-loci [23], 7-loci [24] or even 10-loci [25] based protocols, albeit in the latter study all samples were APAand PSA-positive. It seems that the greater is the complexity of the Y-STR profile the greater is the difficulty to obtain complete DNA profiles but the greater is the certainty of assailant identification.…”

Section: Discussionsupporting

confidence: 76%

Relationship of spermatoscopy, prostatic acid phosphatase activity and prostate-specific antigen (p30) assays with further DNA typing in forensic samples from rape cases

Romero-Montoya¹,

Martínez-Rodríguez²,

Pérez³

et al. 2011

Forensic Science International

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Section: Discussionsupporting

confidence: 76%

Relationship of spermatoscopy, prostatic acid phosphatase activity and prostate-specific antigen (p30) assays with further DNA typing in forensic samples from rape cases

Romero-Montoya¹,

Martínez-Rodríguez²,

Pérez³

et al. 2011

Forensic Science International

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“…Overall, these suggest that STR DNA typing is more sensitive than Sg testing and that DNA profiles of the semen source can still be obtained despite a negative semen test but with the risk of only recovering partial profiles. This has implications for DNA typing not only of samples with trace amounts of semen but also for rape cases in which the assailant failed to ejaculate (Soares-Vieira et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Integrating presumptive and confirmatory semen tests into DNA profiling of sexual assault evidence: a Philippine example

Rodriguez

Calacal

Laude

et al. 2019

Egypt J Forensic Sci

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Background: In sexual assault casework, the detection of semen on a sample supports an allegation of sexual contact and indicates the presence of DNA from a male source. Ideally, techniques employed should demonstrate high sensitivity and specificity and should be compatible with standard DNA typing procedures. To contribute recommendations for routine DNA tests of sexual assault cases in the Philippines, we evaluated the RSID TM-Semen, an immunochromatographic test for human semenogelin (Sg), in parallel with the alternate light source (ALS) method using Mini BLUMAXX™ III and the acid phosphatase (AP) test using Seminal Fluid DISCHAPS TM. Results: The ALS detected semen diluted until 5% (v/v) fluid, whereas the AP assay was sensitive down to 0.5% (v/v). Non-blood-containing stains were visible under blue light except on dark-colored cloth. All semen-containing fluids including post-ejaculatory urine tested positive for AP and Sg. RSID TM-Semen was sensitive down to 0.5 nL of semen; did not cross-react with blood, saliva, and female urine; and successfully detected Sg on the majority of post-coital samples tested. Complete short tandem repeat (STR) profiles of the semen donors were generated for most samples incubated in the RSID TM-Universal Buffer (UB). However, we report an extraction efficiency of 15% for UB which can lead to partial profiles in already compromised samples. Conclusion: We recommend the use of ALS in visualizing stains present on surfaces and the AP test on bloodstained materials. These presumptive tests should be followed by RSID TM-Semen test to confirm the presence of semen prior to DNA profiling.

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“…The individuals donated the samples pre vasectomy and after 20 ejaculations or after 3 to 4 months post-vasectomy surgery. The Ethics Committee for Research Project Analysis nº1022/02 approved the study [4] .…”

Section: Methodsmentioning

confidence: 99%

Stains of ejaculated pre and post-vasectomy: Purity and sufficient quantity of recovered DNA after 10 years of storage

Mautoni

Astolphi

Mello

et al. 2015

Forensic Science International: Genetics Supplement Series

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Vasectomy is a widely used method of contraception. The absence of spermatozoa in the ejaculate of azoospermic individuals increases the difficulty of extracting DNA for analysis, especially if the biological samples are in the form of spots and stored for years. The aim of this study is to evaluate the possibility of obtaining autosomal and Y-STR profiles from stains containing the ejaculate of individuals pre and post-vasectomy, stored in cotton fabric for a period of 10 years. Samples from 28 (twenty eight) individuals: semen pre-vasectomy and seminal fluid (post-vasectomy) stains, stored from 2004 to 2014 at room temperature were used. Three cutouts of 1.5 cm in diameter (punch) were taken from central region of each spot. It was used the QIAamp DNA Mini kit (Qiagen) to extract DNA with an adapted protocol followed by quantification by Nanodrop equipment. Genetic profiles were obtained using ESI17PowerPlex R (Promega). Capillary electrophoresis was performed on the ABI PRISM 3500XL DNA Analyzer. The DNA extraction was successful in all punch analyzed, and its concentration ranged from 1.1ng/uL to 23.3ng/uL in the pre-vasectomy and from 0.8ng/uL to 7 ng/uL in postvasectomy punch. The samples had suitable purity, reflecting the presence of few contaminants. Currently, the techniques of DNA amplification are extremely sensitive and capable of assessing human DNA in small quantities generating partial or full profiles, trespassing situations such as long storage periods in cotton fabric. These data can be used as a benchmark for forensic analysis in cases of semen analysis. IntroductionThe need to obtain genetic profiles to be added to DNA databank is a recent reality in Brazil, since only in 2012 the legislative started to regulate the creation and the use of the National Bank of Genetic Profiles (NBGP), which counts with CODIS technology [1]. It is well known that because of the nature of the crime, sexual assault tends to leave traces that allow the identification of the perpetrator in about 50% of cases [2], and due to the possibility of additional offenses by the criminal, it is recommended to insert the genetic profiles generated in DNA banks [3]. In parallel, there are situations where the only materials available for analysis are traces, such as semen stains on the victim's clothing. The absence of spermatozoa in the ejaculate of azoospermic individuals increases the difficulty of extracting DNA for profile analysis, especially if the biological samples are impregnated on fabric and stored for years. With the launch of NBGP, the analysis of cases without suspects lead to the necessity of generating autosomal genetic profiles for sex offenders. The aim of this study was to evaluate the possibility of obtaining autosomal and Y-STR profiles from ejaculate stains of 28 pre and post-vasectomy individuals stored for 10 years in cotton fabrics.

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Y‐STRs in Forensic Medicine: DNA Analysis in Semen Samples of Azoospermic Individuals

Cited by 20 publications

References 22 publications

Relationship of spermatoscopy, prostatic acid phosphatase activity and prostate-specific antigen (p30) assays with further DNA typing in forensic samples from rape cases

Relationship of spermatoscopy, prostatic acid phosphatase activity and prostate-specific antigen (p30) assays with further DNA typing in forensic samples from rape cases

Integrating presumptive and confirmatory semen tests into DNA profiling of sexual assault evidence: a Philippine example

Stains of ejaculated pre and post-vasectomy: Purity and sufficient quantity of recovered DNA after 10 years of storage

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