Y-STR typing in forensic analysis

Wurmb‐Schwark, Nicole von; Petermann, Sebastian; Wegener, R.

doi:10.1016/s0531-5131(02)00591-5

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Thus, males in the same lineage will possess identical Y-STR haplotypes allowing for the determination of a missing person's male haplotype by typing male relatives. Finally, Y-STR analysis can provide additional discriminatory power when combined with (often partial) autosomal STR profile results [24]–[26].…”

Section: Introductionmentioning

confidence: 99%

An Ultra-High Discrimination Y Chromosome Short Tandem Repeat Multiplex DNA Typing System

Hanson

Ballantyne

2007

PLoS ONE

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In forensic casework, Y chromosome short tandem repeat markers (Y-STRs) are often used to identify a male donor DNA profile in the presence of excess quantities of female DNA, such as is found in many sexual assault investigations. Commercially available Y-STR multiplexes incorporating 12–17 loci are currently used in forensic casework (Promega's PowerPlex® Y and Applied Biosystems' AmpFlSTR® Yfiler®). Despite the robustness of these commercial multiplex Y-STR systems and the ability to discriminate two male individuals in most cases, the coincidence match probabilities between unrelated males are modest compared with the standard set of autosomal STR markers. Hence there is still a need to develop new multiplex systems to supplement these for those cases where additional discriminatory power is desired or where there is a coincidental Y-STR match between potential male participants. Over 400 Y-STR loci have been identified on the Y chromosome. While these have the potential to increase the discrimination potential afforded by the commercially available kits, many have not been well characterized. In the present work, 91 loci were tested for their relative ability to increase the discrimination potential of the commonly used ‘core’ Y-STR loci. The result of this extensive evaluation was the development of an ultra high discrimination (UHD) multiplex DNA typing system that allows for the robust co-amplification of 14 non-core Y-STR loci. Population studies with a mixed African American and American Caucasian sample set (n = 572) indicated that the overall discriminatory potential of the UHD multiplex was superior to all commercial kits tested. The combined use of the UHD multiplex and the Applied Biosystems' AmpFlSTR® Yfiler® kit resulted in 100% discrimination of all individuals within the sample set, which presages its potential to maximally augment currently available forensic casework markers. It could also find applications in human evolutionary genetics and genetic genealogy.

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Section: Introductionmentioning

confidence: 99%

An Ultra-High Discrimination Y Chromosome Short Tandem Repeat Multiplex DNA Typing System

Hanson

Ballantyne

2007

PLoS ONE

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“…STRs are highly polymorphic, easily scored, and can be derived from a tiny amount of biological material. STRs have been widely used for clinical diagnostics, 1,2 population genetics, 3 sexual testing, 4 forensics, [5][6][7][8][9][10] paternity testing and individual identification. [11][12][13] STR analysis comprises selective amplification of target DNA using polymerase chain reaction (PCR), then purification and detection of PCR products.…”

Section: Introductionmentioning

confidence: 99%

The discrimination of three genotypes in STR locus D5S818 based on ultraviolet spectroscopy-chemical pattern recognition

Dou

et al. 2012

Anal. Methods

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A simple, sensitive and low-cost approach for genotyping of a short tandem repeat (STR) based on ultraviolet spectroscopy (UVS)-chemical pattern recognition is presented. Genotypes 10-11, 11-11 and 11-12 of STR locus D5S818 are selected as the objective subjects. Through optimized polymerase chain reaction and ultraviolet spectrum measurement, ultraviolet spectra of all samples are obtained. Based on principal discriminant variate (PDV) and discriminant partial least squares (DPLS), PDV and DPLS models are established by using ultraviolet spectra as recognition variables. By optimizing the weight parameter l in PDV algorithms and the number of latent variables in DPLS algorithms, optimal PDV and DPLS models are obtained. The two optimal models have good separability and robustness, as well as excellent prediction accuracy, which provides a methodological support for establishing ultraviolet spectrum-based classification for all genotypes of D5S818 locus and other STR loci.

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Y-STR typing in forensic analysis

Cited by 2 publications

References 6 publications

An Ultra-High Discrimination Y Chromosome Short Tandem Repeat Multiplex DNA Typing System

An Ultra-High Discrimination Y Chromosome Short Tandem Repeat Multiplex DNA Typing System

The discrimination of three genotypes in STR locus D5S818 based on ultraviolet spectroscopy-chemical pattern recognition

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