Y-STR analysis of digital and/or penile penetration cases with no detected spermatozoa

McDonald, Andrew J.; Jones, Emma; Jw, Lewis; O'Rourke, P.

doi:10.1016/j.fsigen.2014.10.015

Cited by 33 publications

(19 citation statements)

References 9 publications

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“…Another study indicated that more than 90% of the male DNA initially present in simulated sexual-assault samples was lost after differential DNA extraction [28]. Current quantification methods based on q-PCR cannot reliably determine which samples will yield a DNA profile [2]. STR kits were previously shown to be more sensitive than real-time PCR approaches in terms of DNA quantitation [29].…”

Section: Discussionmentioning

confidence: 99%

“…Other methods (e.g. mitochondrial or Y-chromosomal DNA analysis) have restricted applications; for example Y-STR analyses are highly valuable for investigations of sexually motivated crimes in which the minor male proportion in DNA mixtures frequently remains undetected in standard analyses [1,2]. These markers trace male cells even when long periods of time have passed since the assault [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Validation of a combined autosomal/Y-chromosomal STR approach for analyzing typical biological stains in sexual-assault cases

Purps

Geppert

Nagy

et al. 2015

Forensic Science International: Genetics

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DNA testing is an established part of the investigation and prosecution of sexual assault. The primary purpose of DNA evidence is to identify a suspect and/or to demonstrate sexual contact. However, due to highly uneven proportions of female and male DNA in typical stains, routine autosomal analysis often fails to detect the DNA of the assailant. To evaluate the forensic efficiency of the combined application of autosomal and Y-chromosomal short tandem repeat (STR) markers, we present a large retrospective casework study of probative evidence collected in sexual-assault cases. We investigated up to 39 STR markers by testing combinations of the 16-locus NGMSElect kit with both the 23-locus PowerPlex Y23 and the 17-locus Yfiler kit. Using this dual approach we analyzed DNA extracts from 2077 biological stains collected in 287 cases over 30 months. To assess the outcome of the combined approach in comparison to stand-alone autosomal analysis we evaluated informative DNA profiles. Our investigation revealed that Y-STR analysis added up to 21% additional, highly informative (complete, single-source) profiles to the set of reportable autosomal STR profiles for typical stains collected in sexual-assault cases. Detection of multiple male contributors was approximately three times more likely with Y-chromosomal profiling than with autosomal STR profiling. In summary, 1/10 cases would have remained inconclusive (and could have been dismissed) if Y-STR analysis had been omitted from DNA profiling in sexual-assault cases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Validation of a combined autosomal/Y-chromosomal STR approach for analyzing typical biological stains in sexual-assault cases

Purps

Geppert

Nagy

et al. 2015

Forensic Science International: Genetics

View full text Add to dashboard Cite

show abstract

“…Although, in numerous cases, jurisdictions make use of the benefits that lineage marker analysis can bring, they do however have strong limitations in forensic applications, specifically, conclusions may not be drawn on the individual level as would be otherwise desirable [149][150][151]. Although haplotype markers usually are not included as standard markers in police databases, similarly to the Internet-based STR information resource [9,152], haplotype databases have also been designed to store haplotypes from global populations.…”

Section: Brief History Of Uniparental Lineage Markersmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The importance of biological traces and evidences related to a criminal matter has been recognized for a long time. The examination of the expression of genetic polymorphism has been an integral part of the multidisciplinary field of medico-legal autopsy for over a century. Since the initial application of blood group antigens for personalization of a putative perpetrator in a murder case, the discipline of forensic genetics has evolved as a standard of forensic sciences. The real breakthrough, the application of molecular tools and processes for the in-vitro replication of genetic substances, has increasingly allowed the exploitation of advances of molecular genetics for both forensic and criminal investigations. Although there are certainly many more applications and scientific fields in the medico-legal arena, the relatively fast progress of genetics, which has accelerated recently with state-of-art technologies, can provide ever more relevant information in relation to a corpse or the cause and manner that resulted in the corpse for autopsy. This topic concerns the currently accepted forensic DNA technology, and the last section reviews commonly used markers for nuclear and mitochondrial DNA analysis as well as ongoing research. This review also focuses on the increasingly important non-human sources of DNA, and shortly covers the main aspects of animal forensic DNA examination.

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“…Some work has been carried out on the presence of male DNA profiles in casework samples and on internal vaginal swabs . Here, we expand on the current knowledge.…”

mentioning

confidence: 91%

A Study of the Background Levels of Male DNA on Underpants Worn by Females

Murphy

Kenna

Flanagan

et al. 2019

Journal of Forensic Sciences

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This study is the first to examine the background level of male DNA on underpants worn by females in the absence of sexual contact. Here, we examined 103 samples from the inside front of underpants from 85 female volunteers. Samples were examined for the presence of male DNA using NGM SElect and PowerPlex Y23 kits. Only five samples gave a “complete” Y‐STR profile, even though 83.5% of our volunteers cohabited with a male. In all cases where a partner reference sample was available, the Y‐STR profile matched the cohabiting partner. We have demonstrated that a Y‐STR profile is not expected on the inside front of underpants worn by females after social contact alone. The results of this study are informative for evaluating the significance of a Y‐STR profile on underpants in cases of alleged sexual assault.

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Y-STR analysis of digital and/or penile penetration cases with no detected spermatozoa

Cited by 33 publications

References 9 publications

Validation of a combined autosomal/Y-chromosomal STR approach for analyzing typical biological stains in sexual-assault cases

Validation of a combined autosomal/Y-chromosomal STR approach for analyzing typical biological stains in sexual-assault cases

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

A Study of the Background Levels of Male DNA on Underpants Worn by Females

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