Y chromosome visibility in quinacrine-stained human spermatozoa

Roberts, A. M.; Goodall, Harry W.

doi:10.1038/262493a0

Cited by 22 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly to the Y chromosome, disomy 1 or 9 was found in 1-2% of the sperm cells. A disomy rate of 1-2% for each individual chromosome, however, would result in a total of about 35% hyperhaploid sperm cells, an extremely high value that raised some doubt about the reliability of the methods (Roberts and Goodall 1976).…”

Section: Martina Guttenbach · Wolfgang Engel · Michael Schmidmentioning

confidence: 99%

Analysis of structural and numerical chromosome abnormalities in sperm of normal men and carriers of constitutional chromosome aberrations. A review

Engel²,

1997

View full text Add to dashboard Cite

Sperm chromosome analysis offers the opportunity to gather information about the origin of chromosome aberrations in human germ cells. Over the last 20 years more than 20,000 sperm chromosome complements from normal donors and almost 6000 spermatozoa from men with constitutional chromosome aberrations (inversions, translocations) have been analyzed for structural and numerical chromosome abnormalities, as well as for segregation of the constitutional chromosome aberrations after the sperm had penetrated hamster oocytes. On the other hand, it took only 6 years to screen more than 3 million mature spermatozoa from healthy probands for disomy rates of 20 autosomes (chromosomes 19 and 22 not evaluated) and the sex chromosomes, and for diploidy rates by in situ hybridization techniques. In the present paper the results arising from both methods are compiled and compared.

show abstract

Section: Martina Guttenbach · Wolfgang Engel · Michael Schmidmentioning

confidence: 99%

Analysis of structural and numerical chromosome abnormalities in sperm of normal men and carriers of constitutional chromosome aberrations. A review

Engel²,

1997

View full text Add to dashboard Cite

show abstract

“…Questions have been raised on optical grounds about the validity of inferring the presence of two Y chromosomes in quinacrine-stained spermatozoa displaying two fluorescent spots or F-bodies (Roberts and Goodall, 1976). The optical attenuation coefficient is sufficiently great that Y chromosomes located deep in the head of spermatozoa would not be detected and other chromosomes and artifacts fluorescing less brightly than the Y located near the surface would be detected.…”

Section: Estimation Of Rmentioning

confidence: 99%

Incidence of 47,XYY males: Implications of the production of 47,XYY offspring by 47,XYY males

Dorus

Gillespie

1978

Social Biology

View full text Add to dashboard Cite

“…It has also been stated that the scoring of F bodies is a subjective analysis [29]. It is agreed that recognizing F bodies is subjective; however, it does not appear to be any more subjective than scoring Barr bodies or diagnosing malignancies.…”

Section: Dbcp-exposed Individualsmentioning

confidence: 99%

“…It is agreed that recognizing F bodies is subjective; however, it does not appear to be any more subjective than scoring Barr bodies or diagnosing malignancies. [29] suggest that the Y chromosome visibility decreases with Another point of discussion is that some YFF spermatozoa may, in fact, contain…”

Section: Dbcp-exposed Individualsmentioning

confidence: 99%

Analysis of human spermatozoa for Y chromosomal nondisjunction

Kapp

Jacobson

1981

Teratog Carcinog Mutagen

View full text Add to dashboard Cite

The YFF sperm assay, which is a quantification of the incidence of sperm with two fluorescent bodies (YFF = two fluorescent bodies), was performed to measure Y chromosomal nondisjunction. Three categories of human subjects were analyzed: 1) nonexposed, 2) exposed to antineoplastic agents - ie, chemo- and radiation therapy, and 3) dibromochloropropane (DBCP)-exposed. The nonexposed individuals demonstrated a relatively constant mean YFF value of 1.3%, which was consistent with historical controls in this laboratory and with the results of other investigators. Further, a one-way analysis of variance among the means of the control samples revealed no statistical differences either between these men or within each man's samples. The individuals exposed to antineoplastic agents showed a three- to four-fold increase in the incidence of YFF sperm three to six weeks after the initiation of exposure to Adriamycin and X-irradiation. The maximum percentages of YFF per 1,000 sperm for each individual in this exposed group was analyzed by Wilcoxon's distribution free rank sum test using a one-sided alternative. The exposed individuals' maximum YFF percentages were statistically significantly increased when compared to the maximum YFF values of the nonexposed controls. The individuals exposed to the nematocide DBCP also exhibited a statistically significant increase in the number of sperm containing two Y chromosomes as determined by chi-square analysis with one degree of freedom (P less than 0.01). Data presented herein show statistically significant increases in the incidence of double Y chromosomes as measured by the presence of YFF sperm following exposure to Adriamycin, X-irradiation, and DBCP. It is suggested that men who have a history of antineoplastic therapy could be evaluated for evidence of Y-Y nondisjunction with this method. In the event of an increased YFF sperm level, genetic counseling and amniocentesis should be made available to the spouse where pregnancy has occurred. Further, because this procedure measures gametic mutation, is relatively simple, and is noninvasive, it should be considered for inclusion as part of a battery of medical tests for monitoring industrial populations.

show abstract

Y chromosome visibility in quinacrine-stained human spermatozoa

Cited by 22 publications

References 14 publications

Analysis of structural and numerical chromosome abnormalities in sperm of normal men and carriers of constitutional chromosome aberrations. A review

Analysis of structural and numerical chromosome abnormalities in sperm of normal men and carriers of constitutional chromosome aberrations. A review

Incidence of 47,XYY males: Implications of the production of 47,XYY offspring by 47,XYY males

Analysis of human spermatozoa for Y chromosomal nondisjunction

Contact Info

Product

Resources

About