Y‐chromosome short tandem repeats in forensics—Sexing, profiling, and matching male DNA

Roewer, Lutz

doi:10.1002/wfs2.1336

Cited by 43 publications

(33 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, Y-STR haplotypes are shared across paternal relatives and cannot be used to directly identify its source. Without autosomal data, other non-DNA evidence that could provide leads in supporting or negating the contribution of a paternal relative should be considered [ 26 , 27 ]. In all other scenarios when female to male ratio is less than 10:1, using both autosomal and Y-STR profiles will maximize DNA information obtained from a sample.…”

Section: An Integrated System For Dna Testing In Sexual Assault Casesmentioning

confidence: 99%

An integrated system for forensic DNA testing of sexual assault cases in the Philippines

Rodriguez

Laude

Ungria

2021

Forensic Science International: Synergy

View full text Add to dashboard Cite

In the Philippines, more than 7000 cases of sexual assault are reported annually. DNA technology is a powerful tool in identifying assailants. However, it is not routinely used in sexual assault investigations due to insufficient government support to cover the high cost of DNA testing and the absence of a national system for sample collection, handling, storage, and DNA testing of biological evidence. In itself, the nature of sexual assault samples containing DNA mixtures presents challenges to laboratory methods and interpretation of results. The sample recovered from the victim may only contain trace amounts of the assailant’s DNA, may have degraded due to prolonged storage in ambient conditions which is warm and humid in the tropics, or contaminated with inhibitors, such as in anal swabs. Hence, a closer evaluation of the processes of evidence collection and DNA testing is needed to increase the likelihood of success in generating conclusive results. In this paper, we propose an integrated system for DNA testing of biological samples collected from sexual assault victims considering the limitations of resources and the prevailing warm climate. Recommendations in this work should provide basis for formulating national guidelines for DNA analysis in aid of criminal investigations. The proposed scheme can be adopted by forensic DNA laboratories in the Philippines and in other countries facing similar challenges.

show abstract

Section: An Integrated System For Dna Testing In Sexual Assault Casesmentioning

confidence: 99%

An integrated system for forensic DNA testing of sexual assault cases in the Philippines

Rodriguez

Laude

Ungria

2021

Forensic Science International: Synergy

View full text Add to dashboard Cite

show abstract

“…However, the relatively small number of 13 previously identified RM Y-STRs provides limitations for male relative differentiation, particularly regarding closely related men, which limits applications in forensic genetics and genetic genealogy (Roewer, 2019). Empirical studies based on hundreds of male relative pairs showed that these 13 RM Y-STRs allow separation of males related by one, two, three, and four meioses with 27%, 46%, 54%, and 62%, respectively (Adnan et al, 2016), which demonstrates room for improvement.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of novel rapidly mutating Y‐chromosomal short tandem repeat markers

et al. 2020

Self Cite

View full text Add to dashboard Cite

Short tandem repeat polymorphisms on the male‐specific part of the human Y‐chromosome (Y‐STRs) are valuable tools in many areas of human genetics. Although their paternal inheritance and moderate mutation rate (~10−3 mutations per marker per meiosis) allow detecting paternal relationships, they typically fail to separate male relatives. Previously, we identified 13 Y‐STR markers with untypically high mutation rates (>10−2), termed rapidly mutating (RM) Y‐STRs, and showed that they improved male relative differentiation over standard Y‐STRs. By applying a newly developed in silico search approach to the Y‐chromosome reference sequence, we identified 27 novel RM Y‐STR candidates. Genotyping them in 1,616 DNA‐confirmed father–son pairs for mutation rate estimation empirically highlighted 12 novel RM Y‐STRs. Their capacity to differentiate males related by 1, 2, and 3 meioses was 27%, 47%, and 61%, respectively, while for all 25 currently known RM Y‐STRs, it was 44%, 69%, and 83%. Of the 647 Y‐STR mutations observed in total, almost all were single repeat changes, repeat gains, and losses were well balanced; allele length and fathers' age were positively correlated with mutation rate. We expect these new RM Y‐STRs, together with the previously known ones, to significantly improving male relative differentiation in future human genetic applications.

show abstract

“…Because of variation in STRs lenght and high meutation rate, it promoted the genetic polymorphism of STRs markers and therefore useful in human identity testing. The investigation of Y-STRs is a useful reliable marker for personal identity in forensic genetic cases including paternity testing in paternal lineage, historical cases, special cases of the missing person or disaster victim identification involving men, ancestor study, as well as sexual assault with mixed DNA profile between male and female DNA 2,3 . Moreover, Y-STRs analysis can be crucial for sex confirmation in the case of amelogenin dropout 4 .…”

Section: Introductionmentioning

confidence: 99%

Haplotype Profiling of Y-STRs among Northern Population in Thailand

Supakit Khacha-ananda,

Phatcharin Mahawong,

Thida Kaewkod

2021

Indian Journal of Forensic Medicine & Toxicology

View full text Add to dashboard Cite

Background:The investigation of Y-short tandem repeats (Y-STRs) has been widely performed in forensic caseworks. Due to variation between populations, understanding of genetic information within individual a population could provide the database and scientifically reliable results. This study was to investigate allele and haplotype frequencies of Y-STRs among localized people who lived in northern Thailand.Methods: A retrospective descriptive study was conducted by gathering medical reports of Y-STRs typing. The allele frequency and gene diversity as well as haplotype frequency and diversity were calculated. Pairwise genetic distance (R st ) was also calculated based on haplotype pattern by using AMOVA and Multidimensional Scaling (MDS) tools. Results:The result showed that DYS389II, DYS439, and DYS392 represented the highest number of allele patterns. The highest and lowest allele frequency was found to be allele 14 of DYS437 and allele 13 of DYS437, respectively. The highest and lowest gene diversity was observed in DYS389II and DYS437, respectively. One-hundred and sixty different haplotypes were defined where 144 carried a unique haplotype and 16 carried a replicate haplotype. A significant R st value was obtained between the studied population and central Thai population. Conclusion:This study provided genetic database of Y-STRs among localized population in northern region of Thailand. The genetic structure of our subjects also represented the significant close relationship with some other populations in Southeast Asia.

show abstract

Y‐chromosome short tandem repeats in forensics—Sexing, profiling, and matching male DNA

Cited by 43 publications

References 58 publications

An integrated system for forensic DNA testing of sexual assault cases in the Philippines

An integrated system for forensic DNA testing of sexual assault cases in the Philippines

Identification and characterization of novel rapidly mutating Y‐chromosomal short tandem repeat markers

Haplotype Profiling of Y-STRs among Northern Population in Thailand

Contact Info

Product

Resources

About