Y chromosome AZFc microdeletion may not affect the outcomes of ICSI for infertile males with fresh ejaculated sperm

Abstract

Purpose To explore whether the presence of a Y chromosome AZFc microdeletion confers any adverse effect on the outcomes of intracytoplasmic sperm injection (ICSI) with fresh ejaculated sperm. Methods A total of 143 oligozoospermia patients with Y chromosome AZFc microdeletion in ICSI cycles in a fiveyear period were studied. Infertile men with normal Y chromosome in ICSI at the same time-frame were used as controls matched to the study group for age of female, female's body mass index, male's age, infertility duration and number of oocytes retrieved. Retrospective case-control study was used. Results There were no significant differences between groups in clinical outcomes of endometrial thickness, transferred embryos, good embryo rates, implantation rates, biochemical pregnancy rates, clinical pregnancy rates, ectopic pregnancy rates, miscarriage rates, preterm birth rates, the ratio of male and female babies, newborn body height, newborn weight, low birth weight and birth defects (P>0.05). Patients with Y chromosome AZFc microdeletion had a lower fertilization rate (61.8 % vs. 67.8 %, P<0.05) and higher cleaved embryo rate (94.0 % vs. 88.1 %, P<0.05). Conclusions ICSI clinical outcomes for oligozoospermic patients with Y chromosome AZFc microdeletion are basically comparable to that of infertile patients with normal Y chromosomes. The results of ICSI were not affected by the AZFc deletion. Preimplantation genetic diagnosis (PGD) before ICSI for Y chromosome AZFc microdeletion may not be a justifiable regular procedure if the couples didn't care the vertical transmission of Y chromosome deletion.

The sperm quality and clinical outcomes were not affected by sY152 deletion in Y chromosome for oligozoospermia or azoospermia men after ICSI treatment

“…AZFc is the most common deleted region of the Y chromosome in infertile men, it has been thought that the function of this region is predominantly involved in spermatogenesis, and several studies have been reported the relationship between AZFc microdeletion and clinical outcomes (van Golde et al, 2001;Liu et al, 2013). sY152 is one of specific marker in AZFc region, which deletion can lead to oligozoospermia and azoospermia (Muslumanoglu et al, 2005), but litter is known about the relationship between single sY152 deletion and clinical manifestations in IVF/ICSI cycle.…”

“…It is located on the long arm of the Y chromosome (Yq11), which is considered significant for spermatogenesis (Massart et al, 2012;Visser&Repping, 2010;Ghorbian, 2012). Within the Yq11 region, AZF can be divided into three non-overlapping sub-regions: AZFa, AZFb and AZFc, which have been known to be associated with sertoli cell only syndrome (SCOS), spermatogenic maturation arrest at meiosis, and diverse phenotype (including hypospermatogenesis, severe oligozoospermia, azoospermia, or even SCOS) (Liu et al, 2013;Kihaile et al, 2004;van Golde et al, 2001;Zhang et al, 2014), respectively. The incidence of the Y chromosome microdeletion is about 7% among the infertile men, within a range of 3-55% according to previous studies (Pandey et al, 2010;Dai et al, 2012).…”

“…Several investigations have investigated the relationship of AZFc (sY254, sY255) microdeletion and clinical outcomes after intracytoplasmic sperm injection (ICSI) treatment (Liu et al, 2013;van Golde et al, 2001;Wettasinghe et al, 2010). sY152 is one of the STS marker in AZFc region, and previous study have shown that a single sY152 marker deletion can also result in severe oligozoospermia and azoospermia (Muslumanoglu et al, 2005).…”

Correlation between Y chromosome microdeletion and male infertility

“…A study (Kleiman et al, 2002) suggested that, 50% of patients with AZFc deletions could recover fertilization following testis puncture and intracytoplasmic sperm injection (ICSI). Liu et al (2013) suggested that AZFc deletion is more likely to be passed onto male offsprings, but would not increase the risks of infertility among female offsprings. Patients can choose to undergo preimplantation genetic diagnosis followed by sperm implantation in female embryos.…”

Linking Y‐chromosomal short tandem repeat loci to human male impulsive aggression

“…Additionally, there is evidence that SRY downregulates the expression of the estrogen receptor, and in turn attenuates the inhibitory effects of estrogen on male impulsive behavior (Tao et al., 2012). DYS448 and DYS456 loci on the Y chromosome may be linked to SRY (Beltramo, Pena, & Lojo, 2015; Gopinath et al., 2013; Liu, Qiao, Li, Yan, & Chen, 2013). However, whether the two loci affects male impulsive aggressive behavior by above mechanisms needs to be further investigated.…”