Xymedone conjugate with para-aminobenzoic acid. Estimation of hepatoprotective properties

Парфенов, А. А.; Vyshtakalyuk, A. B.; Gumarova, L. F.; Khasanshina, L. R.; Belyaev, G. P.; Назаров, Н. Г.; Kondrashina, D. A.; Galyametdinova, I. V.; Зобов, В. В.; Семенов, В. Э.

doi:10.1007/s11172-019-2704-z

Cited by 4 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Histological Studies: Histological processingpreparation of paraffin sections, staining with Garris hematoxylin (BioVitrum, Russia), alcohol eosin (BioVitrum, Russia) were performed according to standard protocols described in more detail in our previous works [3][4][5][6][7] . Frozen sections were also prepared and stained with Sudan 4 (BioVitrum, Russia) according to standard protocols.…”

Section: Design Of the Experiments Involving The Experimental Animalsmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

2022

IJPSR

View full text Add to dashboard Cite

The present study aimed to study the effect of the drug Xymedon and its conjugate with L-ascorbic acid exhibiting hepatoprotective activity on the apoptosis of rat liver cells against the background of the influence of hepatotoxic agent tetrachloromethane. Though the general effects of Xymedon and its conjugates with various biogenic acids have been studied, the molecular markers affected by the compounds have not yet been established. Experimental methods of investigation included modeling of toxic liver damage by oral administration of carbon tetrachloride to laboratory Wistar rats. To establish the molecular mechanisms of the antiapoptotic effect involved in the hepatoprotective activity of Xymedon and its conjugate with L-ascorbic acid, the level of early apoptosis markers Akt, BAD, BCL-2, p53, Active Caspase-8 Active Caspase-9 in rat liver homogenates was determined by multiplex analysis method using the MagPix system («MerkMillipore», USA). The study showed that Xymedon and its derivative with L-ascorbic acid exhibited an antiapoptotic effect, significantly reducing the number of early apoptosis markers BAD, Active Caspase-9. Also, the derivative with Lascorbic acid reduces the expression of p53. Moreover, it was shown that some biochemical markers are normalized under the influence of the studied compounds. The results obtained have both scientific value, bringing us closer to the true mechanism of action of pyrimidine derivatives, and practical value -the possibility to create an effective hepatoprotective drug with a strong evidence base and degree of study. INTRODUCTION:The liver is the body that plays a key role in the detoxification of exogenous and endogenous toxic substances. Late treatment of mild forms of liver pathologies caused by hepatotoxic substances can lead to more serious up to necrotic damage 1 .

show abstract

Section: Design Of the Experiments Involving The Experimental Animalsmentioning

confidence: 99%

“…Earlier data have shown that conjugation of Xymedon with some bioactive acids leads to an increase in the protective effect of the Xymedon molecule itself [3][4][5] . As a result of exploratory studies, Xymedon conjugate with L-ascorbic acid, among other studied conjugates, has a more pronounced and stable hepatoprotective effect.…”

mentioning

confidence: 99%

Untitled

2022

IJPSR

View full text Add to dashboard Cite

show abstract

“…Гистологическую проводку, приготовление парафиновых срезов, окрашивание гематоксилином Гарриса (BioVitrum, Россия), спиртовым эозином (BioVitrum, Россия) осуществляли по стандартным протоколам, более подробно описанным в работах [4][5][6][7][8][9]. Приготовление замороженных срезов и окрашивание их суданом 4 (BioVitrum, Россия) также выполняли по стандартным протоколам.…”

Section: гистологические исследованияunclassified

“…Несвоевременно начатое лечение легких форм патологий печени, вызванных действием гепатотоксических веществ, может привести к более серьезным, вплоть до некротических повреждений [1]. Объектом настоящего исследования были лекарственный препарат на основе производного Ранее были получены данные, которые показали, что конъюгирование Ксимедона с некоторыми биологически активными кислотами приводит к усилению протекторного действия самой молекулы Ксимедона [3][4][5][6][7][8][9].…”

Section: Introductionunclassified

Anti-Apoptosis Mechanism of the Hepatoprotective Effect of Pyrimidine Derivatives in In Vivo Studies

Parfenov¹,

Vyshtakalyuk²,

Semenov³

et al. 2022

Uch. Zap. Kazan. Univ. Ser. Estestv. Nauki

View full text Add to dashboard Cite

This study investigates the effect of pyrimidine derivatives (Xymedon and its conjugate with L-ascorbic acid, both exhibiting hepatoprotective activity) on the apoptosis of rat liver cells against the background of exposure to carbon tetrachloride as a hepatotoxic agent. The characteristic markers of apoptosis affected by the considered compounds were identified. Modern multiplex analysis of the early apoptosis markers Akt, BAD, BCL-2, p53, Active Caspase-8, and Active Caspase-9 in rat liver homogenates using the MagPix

show abstract