Xylose fermentation to bioethanol production using genetic engineering microorganisms

Komesu, Andrea; Oliveira, Johnatt Allan Rocha de; Neto, João Moreira; Penteado, Eduardo Dellosso; Diniz, A. A. R.; Martins, Luiza Helena da Silva

doi:10.1016/b978-0-12-817953-6.00010-5

Cited by 8 publications

(4 citation statements)

References 36 publications

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“…It is possible that this strain may carry specific transporter(s) and enzyme system involved in the conversion of xylose into L-MA, and may be due to enzyme deficiency involved in the subsequent conversion of L-MA to other products, which cannot be explained at the moment. However, Komesu et al [35] reported that xylose-fermenting bacteria initially take up xylose into the cells through pentose phosphate pathway (PPP) via a specific sugar transporter, after that xylose was converted to D-xylulose 5-phosphate as a precursor intermediate of the PPP by xylose isomerase and xylulose kinase. To understand the pathways involved in the synthesis of L-MA from xylose in the strain H1, we plan to conduct future studies by investigating the enzymes involved in the conversion of xylose to L-MA, transcription of the genes encoding these enzymes via adaptation of the gene expression level [36] and metabolic flux identification of this strain [37].…”

Section: Conversion Of Xylose Derived From Corn Hull To L-ma By a Tropicalis H1mentioning

confidence: 99%

Bioconversion of Untreated Corn Hull into L-Malic Acid by Trifunctional Xylanolytic Enzyme from Paenibacillus curdlanolyticus B-6 and Acetobacter tropicalis H-1

Duong

Ketbot

Phitsuwan

et al. 2021

J. Microbiol. Biotechnol.

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L -Malic acid (L-MA) is widely used in food and non-food products. However, few microorganisms have been able to efficiently produce L-MA from xylose derived from lignocellulosic biomass (LB). The objective of this work is to convert LB into L-MA with the concept of a bioeconomy and environmentally friendly process. The unique trifunctional xylanolytic enzyme, PcAxy43A from Paenibacillus curdlanolyticus B-6, effectively hydrolyzed xylan in untreated LB, especially corn hull to xylose, in one step. Furthermore, the newly isolated, Acetobacter tropicalis strain H1 was able to convert high concentrations of xylose derived from corn hull into L-MA as the main product, which can be easily purified. The strain H1 successfully produced a high L-MA titer of 77.09 g/l, with a yield of 0.77 g/g and a productivity of 0.64 g/l/h from the xylose derived from corn hull. The process presented in this research is an efficient, low-cost and environmentally friendly biological process for the green production of L-MA from LB.

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Section: Conversion Of Xylose Derived From Corn Hull To L-ma By a Tropicalis H1mentioning

confidence: 99%

Bioconversion of Untreated Corn Hull into L-Malic Acid by Trifunctional Xylanolytic Enzyme from Paenibacillus curdlanolyticus B-6 and Acetobacter tropicalis H-1

Duong

Ketbot

Phitsuwan

et al. 2021

J. Microbiol. Biotechnol.

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“…Xylose is the most abundant pentose sugar in hemicellulose and second only to glucose in natural abundance [ 46 ]. Bacteria, filamentous fungi and some yeast species could utilize xylose [ 37 , 47 – 49 ]. Xylose is a promising renewable carbon source for bioproduct generation.…”

Section: Introductionmentioning

confidence: 99%

Second generation Pichia pastoris strain and bioprocess designs

Ergün

Laçın

Çaloğlu

et al. 2022

Biotechnol Biofuels

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Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation. Most of the P. pastoris bioprocess operations has been relying on toxic methanol and glucose feed. In the actual bioeconomy era, for sustainable value-added bioproduct generation, non-conventional yeast P. pastoris bioprocess operations should be extended to low-cost and renewable substrates for large volume bio-based commodity productions. In this review, we evaluated the potential of P. pastoris for the establishment of circular bioeconomy due to its potential to generate industrially relevant bioproducts from renewable sources and waste streams in a cost-effective and environmentally friendly manner. Furthermore, we discussed challenges with the second generation P. pastoris platforms and propose novel insights for future perspectives. In this regard, potential of low cost substrate candidates, i.e., lignocellulosic biomass components, cereal by-products, sugar industry by-products molasses and sugarcane bagasse, high fructose syrup by-products, biodiesel industry by-product crude glycerol, kitchen waste and other agri-food industry by products were evaluated for P. pastoris cell growth promoting effects and recombinant protein production. Further metabolic pathway engineering of P. pastoris to construct renewable and low cost substrate utilization pathways was discussed. Although, second generation P. pastoris bioprocess operations for valorisation of wastes and by-products still in its infancy, rapidly emerging synthetic biology tools and metabolic engineering of P. pastoris will pave the way for more sustainable environment and bioeconomy. From environmental point of view, second generation bioprocess development is also important for waste recycling otherwise disposal of carbon-rich effluents creates environmental concerns. P. pastoris high tolerance to toxic contaminants found in lignocellulosic biomass hydrolysate and industrial waste effluent crude glycerol provides the yeast with advantages to extend its applications toward second generation P. pastoris strain design and bioprocess engineering, in the years to come. Graphical Abstract

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“…In recent years, it has been widely studied to develop xylose fermenting species with genetic modifications in order to increase ethanol production efficiency from lignocellulosic biomass. [14][15][16] Fermentation via yeast is fast and economically effective. 17 Optimum temperature and optimum pH for ethanol fermentation are between 35and 38 C and 4-and 5, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…However, there are some species that can also ferment xylose. In recent years, it has been widely studied to develop xylose fermenting species with genetic modifications in order to increase ethanol production efficiency from lignocellulosic biomass 14‐16 . Fermentation via yeast is fast and economically effective 17 .…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of dilute acid pretreatment of lignocellulosic biomass by response surface methodology to obtain fermentable sugars for bioethanol production

et al. 2021

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Central composite design to optimize sugar recovery from cotton straw and sunflower straw using dilute acid pretreatment was applied. Selected input variables were acid concentration, retention time, and temperature, as well as the response parameter of sugar yield. The optimum pretreatment conditions observed for maximum sugar yield are temperature: 121.7 C, acid concentration: 2.28% (vol/vol), and time: 36.82 minutes for cotton straw; temperature: 87.03 C, and acid concentration: 3.68% (vol/vol), and time: 36.82 minutes for sunflower straw.Maximum sugar concentrations were corresponded to 20 and 17.5 g L −1 under conditions for sunflower and cotton straw, respectively. This study not only investigates the sugar recovery efficiency statistically but also examines the ethanol production efficiency. As a result, the maximum ethanol concentration, ethanol yield, and ethanol productivity of 7.21 g L −1 , 0.41 g g −1 , and 0.10 g L −1 h −1 for cotton straw and 8.05 g L −1 , 0.40 g g −1 , and 0.11 g L −1 h −1 for sunflower straw were achieved via fermentation with Saccharomyces cerevisiae, respectively. The output of this study is important for the commercialization of bioprocesses that enable the conversion of the lignocellulosic waste matrix into high value-added products in a biorefinery concept.

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Xylose fermentation to bioethanol production using genetic engineering microorganisms

Cited by 8 publications

References 36 publications

Bioconversion of Untreated Corn Hull into L-Malic Acid by Trifunctional Xylanolytic Enzyme from Paenibacillus curdlanolyticus B-6 and Acetobacter tropicalis H-1

Bioconversion of Untreated Corn Hull into L-Malic Acid by Trifunctional Xylanolytic Enzyme from Paenibacillus curdlanolyticus B-6 and Acetobacter tropicalis H-1

Second generation Pichia pastoris strain and bioprocess designs

Statistical optimization of dilute acid pretreatment of lignocellulosic biomass by response surface methodology to obtain fermentable sugars for bioethanol production

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