Xylose and cellulose fractionation from corncob with three different strategies and separate fermentation of them to bioethanol

Chen, Yefu; Dong, Boyu; Qin, Weijun; Xiao, Dongguang

doi:10.1016/j.biortech.2010.03.132

Cited by 81 publications

(37 citation statements)

References 24 publications

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“…The column temperature was fixed at 65 °C, and the mobile phase had a flow rate of 0.6 mL/min. The amount of ethanol was quantified as previously reported (Chen et al, 2010).…”

Section: Analytical Methods and Calculationsmentioning

confidence: 99%

“…The liquid seed of C. shehatae was subcultured in fresh culture medium of the same composition and cultivated for an additional day under the same conditions. S. cerevisiae liquid inoculum was grown in a medium containing 40 g/L glucose, 3 g/L yeast extract, 5 g/L peptone, and 2 g/L (NH 4 ) 2 SO 4 at 30 °C (Chen et al, 2010). The other cultivation details were the same as those used for C. shehatae.…”

Section: Seed Culture Of Microorganismsmentioning

confidence: 99%

“…Fermentation medium was inoculated with 10% (v/v) cultures of C. shehatae. The enzymatic hydrolysate was fermented at 28 °C in a 250-mL Erlenmeyer flask containing 100 mL of the culture medium with shaking at 160 rpm for 72 h (Chen et al, 2010). Liquid samples were obtained for ethanol determination.…”

Section: Ethanol Fermentation Of Hemicellulose Enzymatic Hydrolysate mentioning

confidence: 99%

“…Samples were obtained every 12 h at regular intervals, and the solid substrate was removed by centrifugation. The supernatant was used for ethanol determination, and the cellulose content of the remaining solid substrate was measured (Chen et al, 2010). All experiments were performed in triplicate.…”

Section: Ssf By S Cerevisiaementioning

confidence: 99%

See 3 more Smart Citations

Enhanced enzymatic xylose/cellulose fractionation from alkaline liquor-pretreated corn cob by surfactant addition and separate fermentation to bioethanol

Chen¹,

Zhang²,

Zhang³

et al. 2014

Turk J Biol

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Xylanase xylose/cellulose fractionation can efficiently improve the utilization of xylose and cellulose. Improvement of xylanase performance during hemicellulose hydrolysis can increase xylose/cellulose fractionation efficiency. To utilize xylose and cellulose efficiently, an enzymatic xylose/cellulose fractionation and a separate fermentation bioethanol process were performed. Alkaline liquorpretreated corn cob was subjected to xylose/cellulose fractionation with xylanase in the presence or absence of the following mixed surfactants: polyoxyethylene(20)-sorbitan-monooleate (0.15% v/v), polyethylene glycol 6000 (0.15% w/v), bovine serum albumin (0.15% w/v), and rhamnolipid (0.005% v/v). The hemicellulose hydrolysis yield (HHY) in the presence of mixed surfactants was 70.5%, which was 66% higher than that of the control (no surfactants added). Optimization of other hydrolysis conditions, such as pH, temperature, liquid-to-solid ratio, xylanase loading, and incubation time, increased the HHY to 86.62%. The xylose in the enzymatic hydrolysate was fermented to ethanol by Candida shehatae, and the cellulose remaining in the solid residues was converted to ethanol by simultaneous saccharification and fermentation with Saccharomyces cerevisiae. In total, 40.67% of the hemicellulose and 76.14% of the cellulose in the raw corn cob was converted to ethanol.

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“…The column temperature was fixed at 65 °C, and the mobile phase had a flow rate of 0.6 mL/min. The amount of ethanol was quantified as previously reported (Chen et al, 2010).…”

Section: Analytical Methods and Calculationsmentioning

confidence: 99%

Section: Seed Culture Of Microorganismsmentioning

confidence: 99%

Section: Ethanol Fermentation Of Hemicellulose Enzymatic Hydrolysate mentioning

confidence: 99%

Section: Ssf By S Cerevisiaementioning

confidence: 99%

See 2 more Smart Citations

Enhanced enzymatic xylose/cellulose fractionation from alkaline liquor-pretreated corn cob by surfactant addition and separate fermentation to bioethanol

Chen¹,

Zhang²,

Zhang³

et al. 2014

Turk J Biol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another challenging aspect in bioethanol technology is bioconversion of hexoses and pentoses both to ethanol [8]. Unfortunately, there are limited microorganisms that can proficiently convert both of these sugars to ethanol.…”

Section: Introductionmentioning

confidence: 99%