Xylitol production by yeasts isolated from rotting wood in the Galápagos Islands, Ecuador, and description of Cyberlindnera galapagoensis f.a., sp. nov.

Abstract

This study evaluated D-xylose-assimilating yeasts that are associated with rotting wood from the Galápagos Archipelago, Ecuador, for xylitol production from hemicellulose hydrolysates. A total of 140 yeast strains were isolated. Yeasts related to the clades Yamadazyma, Kazachstania, Kurtzmaniella, Lodderomyces, Metschnikowia and Saturnispora were predominant. In culture assays using sugarcane bagasse hemicellulose hydrolysate, Candida tropicalis CLQCA-24SC-125 showed the highest xylitol production, yield and productivity (27.1 g L(-1) xylitol, Y p/s (xyl) = 0.67 g g(-1), Qp = 0.38 g L(-1). A new species of Cyberlindnera, strain CLQCA-24SC-025, was responsible for the second highest xylitol production (24 g L(-1), Y p/s (xyl) = 0.64 g g(-1), Qp = 0.33 g L(-1) h(-1)) on sugarcane hydrolysate. The new xylitol-producing species Cyberlindnera galapagoensis f.a., sp. nov., is proposed to accommodate the strain CLQCA-24SC-025(T) (=UFMG-CM-Y517(T); CBS 13997(T)). The MycoBank number is MB 812171.

Production and purification of xylitol by Scheffersomyces amazonenses via sugarcane hemicellulosic hydrolysate

“…nov. CLQCA-24SC-025, from the Galápagos Archipelago, Ecuador Guaman-Burneo, Dussan. 50 The authors reported similar results (Y P/S = 0.64 g g −1 , Q P = 0.33 g L −1 h −1 , η = 70.58%) in the fermentation of hemicellulosic hydrolysate from sugarcane bagasse to those obtained in the present work.…”

Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production

“…In addition to acetic acid, the hydrolysate may contain other inhibitors such as furfural and HMF, which combined can affect yeast growth and fermentation (Almeida et al 2008;Deparis et al 2017). Since hydrolysate tolerance is species and strain-dependent (Modig et al 2008), and since many studies have already shown that C. tropicalis can produce xylitol with high yields and productivities on fermentative medium containing hydrolysates (Cadete et al 2012;Guamán-Burneo et al 2015;Mattam et al 2016;Dalli et al 2017), a C. tropicalis strain isolated by our group (C. tropicalis CMAA1716 strain, unpublished data) was used as a control in this experiment.…”

“…Among those, yeasts have been reported to be the most efficient xylitol producers (Albuquerque et al 2014;Pal, Mondal and Sahoo 2016). Previous studies involving approximately 40 species, belong to 18 different yeast genera, have identified Candida guilliermondii (currently Meyerozyma guilliermondii (Kurtzman and Suzuki 2010)) and Candida tropicalis as prominent xylitol producers, with production yields up to 0.59 g/g and 0.67 g/g, respectively (Barbosa et al 1988;Guamán-Burneo et al 2015). Recently, new species described as Cyberlindnera galapagoensis, Scheffersomyces amazonensis and Spathaspora spp.…”

“…Recently, new species described as Cyberlindnera galapagoensis, Scheffersomyces amazonensis and Spathaspora spp. strains have shown a significant ability to produce xylitol from xylose, achieving xylitol yields of 0.50 g/g, 0.75 g/g and 0.13-0.50 g/g of xylose, respectively (Cadete et al 2012(Cadete et al , 2016a(Cadete et al ,2016bGuamán-Burneo et al 2015;Lopes et al 2016). However, optimization of fermentative conditions such as aeration, biomass concentration, media pH and supplementation with nutrients, is necessary in order to obtain high xylitol yields and productivities when biomass hydrolysates are used as a substrate (Rao et al 2006;de Arruda et al 2011;Hernández-Pérez et al 2016;Martini et al 2016;Dalli et al 2017).…”

Evaluation of some lignocellulosic byproducts of food industry for microbial xylitol production by Candida tropicalis

“…forestry residues, are used (Mello Lourenco et al 2014; Kamat et al 2013; Guamán-Burneo et al 2015). It was reported that the yeasts adapted to these environments were expected to have high ability to convert xylose to xylitol.…”