Xylitol Production by Xylose Reductase over producing Recombinant Escherichia coli M15

Ariyan, Manikandan; Sivakumar, U.

doi:10.29321/maj.2019.000247

Cited by 4 publications

(3 citation statements)

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“…C. tropicalis isolate GRA1 is a comparison isolate, which has the gene sequences encoding xylose reductase available in the genebank (MF143598.1). Isolate GRA1 is able to reduce xylose to xylitol, which will enter the ethanol fermentation pathway, because it has a xylose reductase gene sequence ( Ariyan & Uthandi 2019 ). The difference in the percentage similarity of the xylose reductase sequences of KBKTI 10.5.1 and Fcs1 isolates to GRA1 can be one of the benchmarks for the success of the genome shuffling that has been carried out.…”

Section: Resultsmentioning

confidence: 99%

Construction of Novel Yeast Strains from Candida tropicalis KBKTI 10.5.1 and Saccharomyces cerevisiae DBY1 to Improve the Performance of Ethanol Production Using Lignocellulosic Hydrolysate

Jamaluddin

Riyanti²,

Mubarik

et al. 2023

tlsr

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Increased consumption of xylose-glucose and yeast tolerance to lignocellulosic hydrolysate are the keys to the success of second-generation bioethanol production. Candida tropicalis KBKTI 10.5.1 is a new isolated strain that has the ability to ferment xylose. In contrast to Saccharomyces cerevisiae DBY1 which only can produce ethanol from glucose fermentation. The research objective is the application of the genome shuffling method to increase the performance of ethanol production using lignocellulosic hydrolysate. Mutants were selected on xylose and glucose substrates separately and using random amplified polymorphic DNA (RAPD) analysis. The ethanol production using lignocellulosic hydrolysate by parents and mutants was evaluated using a batch fermentation system. Concentrations of ethanol, residual sugars, and by-products such as glycerol, lactate and acetate were measured using HPLC machine equipped with Hi-plex H for carbohydrate column and a refraction index detector (RID). Ethanol produced by Fcs1 and Fcs4 mutants on acid hydrolysate increased by 26.58% and 24.17% from parent DBY1, by 14.94% and 21.84% from parent KBKTI 10.5.1. In contrast to the increase in ethanol production on alkaline hydrolysate, Fcs1 and Fcs4 mutants only experienced an increase in ethanol production by 1.35% from the parent KBKTI 10.5.1. Ethanol productivity by Fcs1 and Fcs4 mutants on acid hydrolysate reached 0.042 g/L/h and 0.044 g/L/h. The recombination of the genomes of different yeast species resulted in novel yeast strains that improved resistance performance and ethanol production on lignocellulosic hydrolysates.

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Section: Resultsmentioning

confidence: 99%

Construction of Novel Yeast Strains from Candida tropicalis KBKTI 10.5.1 and Saccharomyces cerevisiae DBY1 to Improve the Performance of Ethanol Production Using Lignocellulosic Hydrolysate

Jamaluddin

Riyanti²,

Mubarik

et al. 2023

tlsr

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“…Apart from using a detoxification step, increasing the inoculum concentration could also help in reducing the effects of the inhibitory compounds in the hydrolysate (Felipe et al, 1997). To enhance the production of xylitol, co-substrates such as glucose and glycerol can be supplemented (Kogje and Ghosalkar, 2017;Ariyan and Uthandi, 2019). One of the key elements in the metabolism of D-xylose in yeast is oxygen.…”

Section: Fermentation Strategies In Xylitol Production Fermentative P...mentioning

confidence: 99%

Xylitol: Bioproduction and Applications-A Review

et al. 2022

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Xylitol, a natural compound classified as a sugar alcohol, is found diversely in fruits and vegetables in small quantities. Commercial production of xylitol has expanded due to its health benefits and wide applications as an alternative sweetener in food and pharmaceutical products. Production of xylitol on large scale is industrially being achieved by the chemical method. However, the biotechnological method offers the possibilities of lowered cost and energy compared to the chemical methods. It involves the conversion of xylose to xylitol by microbes or enzymes which is environmentally safe. This review highlights the prospects of the biotechnological method of xylitol production. Various microorganisms that have been used to produce xylitol, the bioprocess parameters, and genetic modifications to increase xylitol yield have been reviewed. In addition, the applications, benefits, and safety concerns to health have been discussed.

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“…These methods have two steps for the introduction of foreign DNA into Pichia cells; first is the preparation of competent yeast, and the second is the transformation of DNA into cells. Previously we cloned and expressed Xr gene in pQE30Xa under the control of T7 promoter in E. coli M15 and used D-xylose and corncob during the fermentation for the production of xylitol in E. coli (Ariyan and Uthandi, 2019). Pichia pastoris, a methylotrophic yeast of ascomycetes class, is popularly used as a host to express heterologous proteins (Lin-Cereghino et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of efficient transformation method for xylose reductase gene integration in Pichia pastoris GS115

2021

MAJ

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Xylitol Production by Xylose Reductase over producing Recombinant Escherichia coli M15

Cited by 4 publications

References 0 publications

Construction of Novel Yeast Strains from Candida tropicalis KBKTI 10.5.1 and Saccharomyces cerevisiae DBY1 to Improve the Performance of Ethanol Production Using Lignocellulosic Hydrolysate

Construction of Novel Yeast Strains from Candida tropicalis KBKTI 10.5.1 and Saccharomyces cerevisiae DBY1 to Improve the Performance of Ethanol Production Using Lignocellulosic Hydrolysate

Xylitol: Bioproduction and Applications-A Review

Evaluation of efficient transformation method for xylose reductase gene integration in Pichia pastoris GS115

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