Xylem development and phloem conductivity in relation to the stem mechanical strength of Paeonia lactiflora

Yang, Yang; Huang, Yiran; Ren, Anqi; Wan, Yingling; Liu, Yan

doi:10.1016/j.jplph.2023.153963

Cited by 3 publications

(2 citation statements)

References 44 publications

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“…The 9 K-S liquid medium was prepared by sterilizing 9 K medium and adding 10.0 g/L sulfur powder separately. To perform scanning electron microscopy (Hitachi SU8010, Japan), the samples were subjected to cell fixation, gradient dehydration, critical point drying (Leica EM CPD030, Germany), and gold coating (Hitachi E-1045, Japan) before being observed ( Yang et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%

Genome sequencing and metabolic network reconstruction of a novel sulfur-oxidizing bacterium Acidithiobacillus Ameehan

Wu,

Yuan,

Cheng

et al. 2023

Front. Microbiol.

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Sulfur-oxidizing bacteria play a crucial role in various processes, including mine bioleaching, biodesulfurization, and treatment of sulfur-containing wastewater. Nevertheless, the pathway involved in sulfur oxidation is highly intricate, making it complete comprehension a formidable and protracted undertaking. The mechanisms of sulfur oxidation within the Acidithiobacillus genus, along with the process of energy production, remain areas that necessitate further research and elucidation. In this study, a novel strain of sulfur-oxidizing bacterium, Acidithiobacillus Ameehan, was isolated. Several physiological characteristics of the strain Ameehan were verified and its complete genome sequence was presented in the study. Besides, the first genome-scale metabolic network model (AMEE_WP1377) was reconstructed for Acidithiobacillus Ameehan to gain a comprehensive understanding of the metabolic capacity of the strain.The characteristics of Acidithiobacillus Ameehan included morphological size and an optimal growth temperature range of 37-45°C, as well as an optimal growth pH range of pH 2.0-8.0. The microbe was found to be capable of growth when sulfur and K2O6S4 were supplied as the energy source and electron donor for CO2 fixation. Conversely, it could not utilize Na2S2O3, FeS2, and FeSO4·7H2O as the energy source or electron donor for CO2 fixation, nor could it grow using glucose or yeast extract as a carbon source. Genome annotation revealed that the strain Ameehan possessed a series of sulfur oxidizing genes that enabled it to oxidize elemental sulfur or various reduced inorganic sulfur compounds (RISCs). In addition, the bacterium also possessed carbon fixing genes involved in the incomplete Calvin-Benson-Bassham (CBB) cycle. However, the bacterium lacked the ability to oxidize iron and fix nitrogen. By implementing a constraint-based flux analysis to predict cellular growth in the presence of 71 carbon sources, 88.7% agreement with experimental Biolog data was observed. Five sulfur oxidation pathways were discovered through model simulations. The optimal sulfur oxidation pathway had the highest ATP production rate of 14.81 mmol/gDW/h, NADH/NADPH production rate of 5.76 mmol/gDW/h, consumed 1.575 mmol/gDW/h of CO2, and 1.5 mmol/gDW/h of sulfur. Our findings provide a comprehensive outlook on the most effective cellular metabolic pathways implicated in sulfur oxidation within Acidithiobacillus Ameehan. It suggests that the OMP (outer-membrane proteins) and SQR enzymes (sulfide: quinone oxidoreductase) have a significant impact on the energy production efficiency of sulfur oxidation, which could have potential biotechnological applications.

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Section: Methodsmentioning

confidence: 99%

Genome sequencing and metabolic network reconstruction of a novel sulfur-oxidizing bacterium Acidithiobacillus Ameehan

Wu,

Yuan,

Cheng

et al. 2023

Front. Microbiol.

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“…It has been demonstrated that lignin deposition enhances plant cell walls, provides mechanical strength, and is closely related to resistance to lodging. The role of lignin in stem strength and lodging resistance was extensively investigated in herbaceous peony [144,145]. Ca treatment could increase lignin accumulation and reinforce the mechanical strength in peony stems, whereas ethyl glycol tetraacetic acid (EGTA), an effective Ca 2+ chelator, had a negative role [146,147].…”

Section: Lodging Resistance and Stem Strengthmentioning

confidence: 99%

More or Less: Recent Advances in Lignin Accumulation and Regulation in Horticultural Crops

Wang,

Wu,

Chen

et al. 2023

Agronomy

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Lignin is an important secondary metabolite that maintains the mechanical strength of horticultural plants and enhances their ability to respond to external environmental changes such as biotic and abiotic stresses. However, excessive accumulation of lignin can lead to lignification of horticultural products, reducing their taste quality and nutritional value. Therefore, the lignin content of horticultural products needs to be controlled at a reasonable level, and studying and regulating lignin metabolism is very meaningful work. This article focuses on the synthesis, accumulation, and regulation of lignin in horticultural crops in recent years, provides a systematic analysis of its molecular mechanism and application prospects, and sheds insights into the directions that need further research in the future. This article provides an important basis for the regulation of lignin accumulation and lignification in horticultural crops and proposes new ideas for improving the quality of horticultural crops.

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Identification and Functional Studies on the Role of PlSPL14 in Herbaceous Peony Stem Development

Xu,

Yu,

Tang

et al. 2024

IJMS

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Stem strength plays a crucial role in the growth and development of plants, as well as in their flowering and fruiting. It not only impacts the lodging resistance of crops, but also influences the ornamental value of ornamental plants. Stem development is closely linked to stem strength; however, the roles of the SPL transcription factors in the stem development of herbaceous peony (Paeonia lactiflora Pall.) are not yet fully elucidated. In this study, we obtained and cloned the full-length sequence of PlSPL14, encoding 1085 amino acids. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression level of PlSPL14 gradually increased with the stem development of P. lactiflora and was significantly expressed in vascular bundles. Subsequently, utilizing the techniques of virus-induced gene silencing (VIGS) and heterologous overexpression in tobacco (Nicotiana tabacum L.), it was determined that PlSPL14-silenced P. lactiflora had a thinner xylem thickness, a decreased stem diameter, and weakened stem strength, while PlSPL14-overexpressing tobacco resulted in a thicker xylem thickness, an increased stem diameter, and enhanced stem strength. Further screening of the interacting proteins of PlSPL14 using a yeast two-hybrid (Y2H) assay revealed an interactive relationship between PlSPL14 and PlSLR1 protein, which acts as a negative regulator of gibberellin (GA). Additionally, the expression level of PlSLR1 gradually decreased during the stem development of P. lactiflora. The above results suggest that PlSPL14 may play a positive regulatory role in stem development and act in the xylem, making it a potential candidate gene for enhancing stem straightness in plants.

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Xylem development and phloem conductivity in relation to the stem mechanical strength of Paeonia lactiflora

Cited by 3 publications

References 44 publications

Genome sequencing and metabolic network reconstruction of a novel sulfur-oxidizing bacterium Acidithiobacillus Ameehan

Genome sequencing and metabolic network reconstruction of a novel sulfur-oxidizing bacterium Acidithiobacillus Ameehan

More or Less: Recent Advances in Lignin Accumulation and Regulation in Horticultural Crops

Identification and Functional Studies on the Role of PlSPL14 in Herbaceous Peony Stem Development

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