Xylaria feejeensis, SRNE2BP a Fungal Endophyte with Biocontrol Properties to Control Early Blight and Fusarium Wilt Disease in Tomato and Plant Growth Promotion Activity

Brooks, Siraprapa; Klomchit, Anthikan; Chimthai, Surangkana; Jaidee, Wuttichai; Bastian, Aaron Christopher

doi:10.1007/s00284-022-02803-x

Cited by 20 publications

(11 citation statements)

References 48 publications

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“…Thus, these two strains were further analyzed for antagonistic potential with a poisoned food assay. The liquidliquid chemical extraction method using ethyl acetate was employed to obtain crude extract from the two fungal endophytes following the technique described in Brooks et al (2022). Brie y, fungal endophytes were cultured in a PDA medium at 28 °C for seven days and mycelium plugs were inoculated into the PDB medium.…”

Section: Poisoned Food Assaymentioning

confidence: 99%

“…The spore of pathogenic fungi C. truncatum, MFLUCC CG-0064 and C. acutatum, MFLUCC CC-0036 were induced by incubation in 1/10 PDA for 25 and 10 days, respectively. Spore solutions were prepared and adjusted to 1×10 6 spores/mL followed the method described in Brooks et al (2022). The 1 µL of fungal crude extract (90 mg/mL) was added to 9 µL of spore solution of pathogenic fungi.…”

Section: Inhibition Of Spore Germination Inhibition Of Hypha Expansio...mentioning

confidence: 99%

“…Inoculated leaves were placed in plastic containers with moist paper towels for ve days. Leaves were cut and stained with Coomassie Brilliant Blue mixture following methods described in Brooks et al (2022). A light microscope was used to observe hyphal coverage on the plant material.…”

Section: Inhibition Of Spore Germination Inhibition Of Hypha Expansio...mentioning

confidence: 99%

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Novel Hypocrealean Fungi from Rhizophoraceae, Neocosmospora mangrovei sp. nov. and Neocosmospora ceriopis sp. nov., and their Antifungal Activity against Anthracnose Pathogen Colletotrichum spp.

Brooks

Klomchit

Calabon

et al. 2022

Preprint

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Neocosmospora is a fungus genus that contains saprobes, endophytes, and pathogens in the family Nectriaceae. Prior to this study, 113 morphological species belonging to this genus have been described and published. In this study, we reported two new species of fungus, Neocosmospora mangrovei (MFLUCC 17–0253; MFLUCC 17–0257) and N. ceriopis (MFLUCC 17–0259) that were isolated from the mangrove habitats in the Southern part of Thailand. Phylogenetic analyses of the combined ITS, EF-1α, RPB2, and LSU regions showed that the new species clustered within the Neocosmospora clade with strong statistical support. Detailed descriptions and illustrations, pairwise homoplasy index (PHI) test, and a phylogenetic tree showing the position of both new species are provided. In addition, both new species showed the potential to be used as biocontrol agents against Colletotrichum spp. The N. mangrovei, MFLUCC 17–0253 exhibited strong antifungal activity against C. truncatum, MFLUCC CG-0064 e.g., 100% of mycelium growth inhibition, 90.83% inhibition of spore germination, and 100% reduction hyphae expansion. The N. ceriopis MFLUCC 17–0259 exhibited antifungal activity against C. acutatum, MFLUCC CC-0036 e.g., 69.39% of mycelium growth inhibition, 79.39% inhibition of spore germination, and 96.42% reduction hyphae expansion. Overall, these findings suggested novel species of Neocosmospora that had the capacity to produce various classes of biometabolites including 2,3-hydro-5-hydroxy-8-methoxy-2,4-dimethylnapthol-[1,2-b]-furan-6,9-dione, 2-methoxy-6-methyl-7-acetonyl-8-hydroxy-1,4-maphthalenedione, 5,8-dihydroxy-7-acetonyl-1,4-naphthalenedione, anhydrojavanicin, and fusarnaphthoquinones that can provide an antimicrobial activity that could have the potential in playing a big role in treating anthracnose disease.

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Section: Poisoned Food Assaymentioning

confidence: 99%

Section: Inhibition Of Spore Germination Inhibition Of Hypha Expansio...mentioning

confidence: 99%

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Novel Hypocrealean Fungi from Rhizophoraceae, Neocosmospora mangrovei sp. nov. and Neocosmospora ceriopis sp. nov., and their Antifungal Activity against Anthracnose Pathogen Colletotrichum spp.

Brooks

Klomchit

Calabon

et al. 2022

Preprint

Self Cite

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“…Leaves were cut and stained with Coomassie Brilliant Blue mixture following methods described in Brooks et al (2022). A light microscope was used to observe hyphal coverage on the plant material.…”

Section: Inhibition Of Spore Germination Inhibition Of Hypha Expansio...mentioning

confidence: 99%

Comparative Analysis of Conventional Optimization Techniques with RSM and ANN Models for Extracting Oil from Sterculia urens Seeds for Biodiesel Applications

Nagarajan

Pandian

Karuppasamy

et al. 2022

Preprint

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In this work, oil was extracted from Sterculia urens (S. urens) seeds in a batch reactor and the parameters affecting this process were optimized. For this study, a suitable solvent for extraction was identified and its solvent to seed meal ratio was determined as petroleum ether solvent and 8:1, respectively. The total oil content of the seed by Soxhlet extraction is 38.9 wt%. Other parameters which affect the oil yield, such as the meal size of seed, extraction temperature and extraction time, were optimized and their values were 0.25 mm, 60°C and 240 min, respectively, to get 38.2 wt% oil. Moreover, Response Surface Methodology (RSM) and Artificial Neural Network (ANN) techniques were compared with conventional extraction and used for predicting the optimum values. The correlation regression coefficient (R2) values for RSM and ANN were 0.822 and 0.99, respectively. The predicted optimum values obtained in both the tools are approximately similar for oil extraction as 0.5 mm meal size, 60°C temperature and 180 min to get optimum yield. Finally, the physiochemical parameters of the oil were determined by standard methods and predicted the properties of biodiesel made from this. Thus these results suggest that the oil obtained can be used as a potential second-generation non-edible feedstock for biodiesel production.

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“…Tomato early blight caused by Alternaria solani is one of the important diseases in tomato cultivation, which can lead to detaching of the leaves, drying of branches, and premature fruit drop, and the resulting fruit yield losses often amount to 50–86% [ 13 , 14 ]. The main chemical agents that have been applied to control tomato early blight include trifloxystrobin, tebuconazole [ 15 ], oxathiapiprolin, and benthiavalicarb [ 16 ], while the currently used biocontrol agents include Bacillus [ 17 ] and Trichoderma [ 18 ], which are still very limited. In this study, A. solani was used as an indicator fungus, and four active compounds were obtained from a Streptomyces fungicidicus SYH3 fermentation broth by a series of separation and purification procedures.…”

Section: Introductionmentioning

confidence: 99%

Identification of Cyclic Dipeptides and a New Compound (6-(5-Hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one) Produced by Streptomyces fungicidicus against Alternaria solani

et al. 2022

Molecules

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As an important microbial resource, Actinomycetes, especially Streptomyces, have important application values in medicine and biotechnology. Streptomyces fungicidicus SYH3 was isolated from soil samples in tomato-growing areas and showed good inhibitory effects on Alternaria solani in tomato. To obtain pure active compounds, SYH3 fermentation broth was subjected to XAD-16 macroporous resin and silica gel column chromatography. Combined with the repeated preparation and separation of preparative high-performance liquid chromatography (HPLC), a total of four monomer compounds were obtained after activity tracking. Compound 4 was identified as a new six-membered lactone ring compound named 6-(5-hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one by 1D and 2D nuclear magnetic resonance (NMR) data and mass spectrometry (MS). The other three active compounds belong to the cyclodipeptide, and their half maximal inhibitory concentration (IC50) values against A. solani were 43.4, 42.9, and 30.6 μg/mL, respectively. Compound 4 significantly inhibited the spore germination and induced swollen and deformed local hyphae of A. solani with an IC50 value of 24.9 μg/mL. Compound 4 also had broad-spectrum antifungal activity and had a good antifungal effect on the tested plant-pathogenic fungi. The modes of action of new compound (4) still require further investigation, representing a novel and effective anti-fungal agent for future application.

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Xylaria feejeensis, SRNE2BP a Fungal Endophyte with Biocontrol Properties to Control Early Blight and Fusarium Wilt Disease in Tomato and Plant Growth Promotion Activity

Cited by 20 publications

References 48 publications

Novel Hypocrealean Fungi from Rhizophoraceae, Neocosmospora mangrovei sp. nov. and Neocosmospora ceriopis sp. nov., and their Antifungal Activity against Anthracnose Pathogen Colletotrichum spp.

Novel Hypocrealean Fungi from Rhizophoraceae, Neocosmospora mangrovei sp. nov. and Neocosmospora ceriopis sp. nov., and their Antifungal Activity against Anthracnose Pathogen Colletotrichum spp.

Comparative Analysis of Conventional Optimization Techniques with RSM and ANN Models for Extracting Oil from Sterculia urens Seeds for Biodiesel Applications

Identification of Cyclic Dipeptides and a New Compound (6-(5-Hydroxy-6-methylheptyl)-5,6-dihydro-2H-pyran-2-one) Produced by Streptomyces fungicidicus against Alternaria solani

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