Xylans inhibit enzymatic hydrolysis of lignocellulosic materials by cellulases

Zhang, Junhua; Tang, Ming; Viikari, Liisa

doi:10.1016/j.biortech.2012.07.010

Cited by 179 publications

(100 citation statements)

References 21 publications

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“…We do not know the susceptibilities of the two cellulases we used in our simulations to competitive inhibition by GAXs or xylooligosaccharides that have been suggested [11][12][13][14][15], but neither of them is a cellobiohydrolase, the type most susceptible to such inhibition. We therefore did not attempt to model such inhibition.…”

Section: Resultsmentioning

confidence: 99%

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In Silico Comparison of the Hemicelluloses Xyloglucan and Glucuronoarabinoxylan in Protecting Cellulose from Degradation

et al. 2015

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Abstract:We used a previously developed simulation model of a plant cell wall and its enzymatic degradation to compare the abilities of two hemicelluloses, glucuronoarabinoxylan (GAX) and xyloglucan (XG), to protect cellulose microfibrils (CMFs) from attack by cellulose-degrading enzymes. Additionally, we investigated the effect of XG abundance on the degradation rate of CMFs in the presence of the same enzymes. Simulations were run using hypothetical cell-wall compositions in which the numbers and arrangement of CMFs and (1,3;1,4)-β-glucan were kept constant, but the proportions of GAX and XG were altered. Scenarios considered walls with low and equal proportions of either GAX or XG, and also low, medium and high proportions of XG in the absence of GAX. The rate of CMF degradation was much lower in walls with GAX than walls with XG, except for early in the simulation when the reverse held, suggesting that XGs were protecting CMFs by competitive inhibition. Increasing XG content reduced both the degradation rate of CMFs and the percent of XG degraded, indicating that activity of enzymes decreased with XG density despite XG being degradable. Glucose oligosaccharide breakdown products were analysed on the basis of the originating polysaccharide and their OPEN ACCESSComputation 2015, 3 337 degree of polymerisation (DP). The presence of GAX as opposed to equal amounts of XG had some significant effects on the amount and profile of breakdown products from XG and CMFs.

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Section: Resultsmentioning

confidence: 99%

“…of cellulases and competitively inhibit them [11][12][13][14][15]. This inhibition was found to be particularly marked for cellobiohydrolases, which are exo-cellulases that successively cleave cellobiose units from the reducing end (type I) or non-reducing end (type II) of cellulose or cellooligosaccharides.…”

mentioning

confidence: 99%

In Silico Comparison of the Hemicelluloses Xyloglucan and Glucuronoarabinoxylan in Protecting Cellulose from Degradation

et al. 2015

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“…The nutritional value of these materials can be enhanced to serve as a medium for microbial growth in the production of bioenergy, such as biogas (Gomez-Tover et al, 2012;He et al, 2008) and bioethanol (Biond et al, 2010;Ko et al 2009;Sun and Cheng 2005). Presence of both lignin and hemicelluloses has shown to be a major barrier in chemical or enzymatic hydrolysis of cellulose (Zhang et al, 2012). Therefore, biomass needs to be pre-treated to alter the lignin and hemicelluloses contents and cellulose fibre crystallinity to enhance enzyme or chemical accessibility and thereby increase the bio-digestibility.…”

Section: Introductionmentioning

confidence: 99%

Chemical Pre-treatment for Enhancing Bio Accessibility of Oat Husk

Gunawardena

Jin²

2016

AJBB

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Agricultural production delivers enormous waste biomass materials, which have very low values for industries, but contributes significant environmental impact on carbon emission. This study was aimed to identify cost-effective chemical technologies to enhance bio-accessibility of cellulose-based agricultural biomass materials, which can be used as carbohydrates for fermentation process. Three chemical methods alkaline, dilute acid and ammonia soaking were investigated for the pretreatment of oat husk, which was used as a cellulose biomass model. Comparison studies of the pretreatment methods were carried out in a series of bench scale tests and biochemical analysis. These chemical pretreatment methods were evaluated by enzymatically hydrolysing the treated oat husk and estimating the mount of glucose produced in the hydrolysate. Experimental results revealed these pretreatment methods are promising for enhancing the enzymatic accessibility of the cellulose biomass materials. Alkaline pretreatment method appeared to demonstrate the best results in terms of enzymatic hydrolysis and glucose yield.

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“…Hemicelluloses play an important role in biomass enzymatic digestion (Xu et al 2012;Zhang et al 2012;Li et al 2013a). Xylan, the major hemicellulosic polysaccharide present in plant cell walls, has a backbone of β-1,4-linked xylose residues and side chains of different substituents (Vázquez et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…However, high-activity xylanase and β-xylosidase should be supplemented in saccharification processes for total hydrolysis of lignocellulosic biomass into monosaccharides because xylan clearly inhibited the hydrolysis of cellulose by cellulase and xylooligomers are stronger inhibitors of cellulase activity than are glucose and cellobiose (Selig et al 2008;Qing et al 2010;Zhang et al 2012). Currently, most biotechnological processes are based on the use of crude enzymes.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Enzymatic Hydrolysis of Miscanthus Xylan using Aspergillus niger, Hypocrea orientalis, and Trichoderma reesei Xylan-degrading Enzymes

Liu

et al. 2014

BioRes

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Xylan-degrading enzymes from Aspergillus niger and Hypocrea orientalis were characterized by enzyme activity assays and protein profiling with SDS-PAGE and LC-MS/MS. The hydrolysis of Miscanthus xylan by xylan-degrading enzymes from A. niger, H. orientalis, and Trichoderma reesei were comparatively studied by HPLC analysis. It was found that the glycoside hydrolase families 10 xylanase was the main xylanase secreted by H. orientalis and A. niger when using corn cob and wheat bran as inducers. Compared to the enzymes from T. reesei,the enzymes from A. niger showed better efficiency in the hydrolysis of Miscanthus xylan into monosaccharides. Nevertheless, the enzymes from H. orientalis were more preferable for the hydrolysis of Miscanthus xylan into xylo-oligosaccharides (XOS), especially xylobiose and xylotriose. Miscanthus xylan degradation was significantly influenced by the activities of β-xylosidase and α-L-arabinofuranosidase. Xylan-degrading enzymes with high ratios of β-xylosidase and α-L-arabinofuranosidase are necessary for the efficient conversion of Miscanthus xylan into monosaccharides. However, xylan-degrading enzymes with low β-xylosidase activity and high α-L-arabinofuranosidase activity were required for producing XOS.

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Xylans inhibit enzymatic hydrolysis of lignocellulosic materials by cellulases

Cited by 179 publications

References 21 publications

In Silico Comparison of the Hemicelluloses Xyloglucan and Glucuronoarabinoxylan in Protecting Cellulose from Degradation

In Silico Comparison of the Hemicelluloses Xyloglucan and Glucuronoarabinoxylan in Protecting Cellulose from Degradation

Chemical Pre-treatment for Enhancing Bio Accessibility of Oat Husk

Comparative Analysis of Enzymatic Hydrolysis of Miscanthus Xylan using Aspergillus niger, Hypocrea orientalis, and Trichoderma reesei Xylan-degrading Enzymes

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