Xylanase supplementation to a wheat-based diet alleviated the intestinal mucosal barrier impairment of broiler chickens challenged byClostridium perfringens

Liu, Dan; Guo, Shuangshuang; Guo, Yuming

doi:10.1080/03079457.2012.684089

Cited by 88 publications

(83 citation statements)

References 51 publications

(69 reference statements)

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“…Probiotics can indirectly suppress the proliferation of pathogenic bacteria, such as C. perfringens (Annett et al, 2002). In addition, xylanase had positive effects on morphometric indices and increased the villus height-to-crypt depth ratio in different segments of the small intestine (Liu et al, 2012), resulting in a larger surface area available for digestion and absorption of nutrients. All these factors facilitated the digestibility of nutrients and improved the digestibility of protein for challenged broilers.…”

Section: Discussionmentioning

confidence: 99%

“…An increase in disaccharidase activity is associated with an increase in the capacity for disaccharide digestion and absorption in the epithelium (Moreno et al, 1995). Liu et al (2012) found that xylanase supplementation improves sucrose digestion in challenged broilers by reducing the number of pathogenic bacteria in the small intestine. In contrast, xylanase decreases the chyme viscosity that may prevent contact between bush border enzymes and their substrates by thickening the unstirred mucosal layer (Wang et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The NE challenge was a modified version of the method described by Liu et al (2012). A broiler C. perfringens type A field strain was isolated from a clinical case of NE at the Iranian Veterinary Culture Center (Mashhad, Iran).…”

Section: Necrotic Enteritis Challengementioning

confidence: 99%

“…The positive effects of xylanase may reduce intestinal functional disorders caused by C. perfringens infection. Liu et al (2012) noted that the xylanase enzyme reduced the impairment of the intestinal mucosal barrier in birds infected by C. perfringens. However, the possible mechanism(s) underlying the influence of xylanase on the nutrient digestibility in subclinical NE of starter broilers fed a wheat-based diet is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens

2017

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This study was conducted to evaluate the effects of xylanase and citric acid (CA) on growth, digesta pH, ileal populations of Clostridium perfringens and lactic acid bacteria, ileal nutrient digestibility, digestive enzyme activity, and mRNA expression of intestinal nutrient transporters in starter broilers challenged with C. perfringens. The experiment was conducted in a 2×2 factorial arrangement with two levels of CA (0 and 30 g/kg) and 2 levels of xylanase (0 and 200 mg/kg). Each of the four dietary treatments was fed to six replicate pens (15 birds/pen) between 0 and 21 d of age. Dietary CA significantly increased ADFI and ADG; meanwhile, xylanase addition led to a substantial reduction in FCR (P＜0.05). No differences in digesta pH, C. perfringens counts, or quantity of lactic acid bacteria were found between the treatments. Xylanase supplementation increased AME values (P＜0.01) and ileal digestibility of CP (P＜0.05) in challenged birds. The inclusion of CA also increased the AME (P＜0.01), and tended to increased ileal CP digestibility (P＝0.085). Xylanase supplementation increased α-amylase, trypsin, and sucrose activity in the jejunum (P＜0.01). Dietary CA significantly increased (P＜0.01) villi length as well as the villus length to crypt depth ratio in jejunum segments. The jejunal mRNA expression of sodium glucose co-transporter 1 (SGLT1) and H + -dependent peptide transporter 1 (PepT1) were upregulated by xylanase supplementation (P＜0.01). The results suggest that dietary CA can promote growth as well as improve intestinal morphology and AME in birds challenged with necrotic enteritis. This study shows that xylanase supplementation improved FCR and AME in birds independent of C. perfringens infection; it also elevated the apparent ileal digestibility of CP, digestive enzyme activities, and mRNA expression of nutrient transporters in challenged birds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Necrotic Enteritis Challengementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens

2017

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“…C. perfringens challenge C. perfringens challenge was based on the model originally developed by Dahiya et al (2005) and modified by Liu et al (2012). C. perfringens type A strain isolated from a clinical case of NE was obtained from China Veterinary Culture Collection Center (Beijing, China).…”

Section: Essential Oil and Enzymesmentioning

confidence: 99%