Xylanase production by Paenibacillus campinasensis BL11 and its pretreatment of hardwood kraft pulp bleaching

Ko, Chun-Han; Lin, Zi-Ping; Tu, Jenn; Tsai, Chien-Chung; Liu, Chia-Chen; Chen, Hsin-Tai; Wang, Tzu-Pin

doi:10.1016/j.ibiod.2009.10.001

Cited by 58 publications

(32 citation statements)

References 28 publications

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“…The xylanase activity was comparable to highly productive Bacillus and Paenibacillus strains cultivated under optimized conditions, suggesting that mixed consortial cultivation on switchgrass is an effective method to generate high levels of xylanase enzymes (18). Celluloytic activity was relatively low, and cultivations on pretreated switchgrass are being explored to enhance the cellulolytic activity of the recovered supernatants.…”

Section: Discussionmentioning

confidence: 90%

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass

Gladden

Allgaier

Miller

et al. 2011

Appl Environ Microbiol

113

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Industrial-scale biofuel production requires robust enzymatic cocktails to produce fermentable sugars from lignocellulosic biomass. Thermophilic bacterial consortia are a potential source of cellulases and hemicellulases adapted to harsher reaction conditions than commercial fungal enzymes. Compost-derived microbial consortia were adapted to switchgrass at 60°C to develop thermophilic biomass-degrading consortia for detailed studies. Microbial community analysis using small-subunit rRNA gene amplicon pyrosequencing and short-read metagenomic sequencing demonstrated that thermophilic adaptation to switchgrass resulted in low-diversity bacterial consortia with a high abundance of bacteria related to thermophilic paenibacilli, Rhodothermus marinus, and Thermus thermophilus. At lower abundance, thermophilic Chloroflexi and an uncultivated lineage of the Gemmatimonadetes phylum were observed. Supernatants isolated from these consortia had high levels of xylanase and endoglucanase activities. Compared to commercial enzyme preparations, the endoglucanase enzymes had a higher thermotolerance and were more stable in the presence of 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]), an ionic liquid used for biomass pretreatment. The supernatants were used to saccharify [C2mim][OAc]-pretreated switchgrass at elevated temperatures (up to 80°C), demonstrating that these consortia are an excellent source of enzymes for the development of enzymatic cocktails tailored to more extreme reaction conditions.

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Section: Discussionmentioning

confidence: 90%

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass

Gladden

Allgaier

Miller

et al. 2011

Appl Environ Microbiol

113

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“…The isolated bacterial strain was grown in medium containing (g l ¡1 ): beechwood xylan, 15 3.0 £ 10 ¡4 ; Na 2 MoO 4 , 1.0 £ 10 ¡3 ), 20 ml; the pH was adjusted to 6.0 using 1 M NaOH. The medium was inoculated with 16-h-old culture (early exponential phase) and incubated at 50°C in an orbital shaker at 150 rpm.…”

Section: Identiwcation Of Microorganism and Culture Conditionsmentioning

confidence: 99%

A novel thermostable xylanase of Paenibacillus macerans IIPSP3 isolated from the termite gut

Dheeran

Nandhagopal

Kumar

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Xylanase is an enzyme in high demand for various industrial applications, such as those in the biofuel and pulp and paper fields. In this study, xylanase-producing microbes were isolated from the gut of the wood-feeding termite at 50°C. The isolated microbe produced thermostable xylanase that was active over a broad range of temperatures (40-90°C) and pH (3.5-9.5), with optimum activity (4,170 ± 23.5 U mg⁻¹) at 60°C and pH 4.5. The enzyme was purified using a strong cation exchanger and gel filtration chromatography, revealing that the protein has a molecular mass of 205 kDa and calculated pI of 5.38. The half-life of xylanase was 6 h at 60°C and 2 h at 90°C. The isolated thermostable xylanase differed from other xylanases reported to date in terms of size, structure, and mode of action. The novelty of this enzyme lies in its high specific activity and stability at broad ranges of temperature and pH. These properties suggest that this enzyme could be utilized in bioethanol production as well as in the paper and pulp industry.

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“…Compared with the results found by Ko et al (2010), xylanase pretreatment at 2.5 IU/g.o.d.p. increased brightness by 4.6% for hardwood kraft pulp using a Kappa factor of 0.1 in the D0 stage of DED bleaching, and xylanase pretreatment at 6 IU/g.o.d.p.…”

Section: Pulp Properties Of Enzymatic Pretreatment After Chlorine Diomentioning

confidence: 56%

Properties of Enzyme Pretreated Wikstroemia sikokiana and Broussonetia papyrifera Bast Fiber Pulps

Lin¹,

Chang²,

Ko³

et al. 2015

BioResources

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Xylanase, pectinase complex, and BL11 pectinase were employed for the pretreatment of gampi and paper mulberry bast fiber pulps prior to chlorine dioxide bleaching. The bleaching efficiencies of the pulps with different enzymatic pretreatments were investigated. Accelerated aging by heat-humidity treatment was also conducted to evaluate yellowing phenomena and to estimate the prevention of brightness reversion (brightness retention) by enzymatic pretreatment. The order of active chlorine required with respect to pretreatment was pectinase complex > xylanase > BL11 pectinase for soda and soda/oxalate gampi pulps and pectinase complex > BL11 pectinase > xylanase for soda and soda/oxalate paper mulberry pulps. Higher brightness retention values were observed for soda/oxalate pulps compared to soda pulps. The brightness retention levels for gampi pulps and mulberry pulps after ClO2 bleaching with enzymatic pretreatment were higher than the levels of ClO2 and NaClO bleaching pulps. Enzymatic treatments were thus able to reduce the usage of ClO2 and to assist in producing photo-stable paper materials for art and artifact-repairing applications. Thus, enzymatic pretreatment of the pulp has the potential to meet world trends and environmental sustainability for pulp and paper industries.

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Xylanase production by Paenibacillus campinasensis BL11 and its pretreatment of hardwood kraft pulp bleaching

Cited by 58 publications

References 28 publications

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass

A novel thermostable xylanase of Paenibacillus macerans IIPSP3 isolated from the termite gut

Properties of Enzyme Pretreated Wikstroemia sikokiana and Broussonetia papyrifera Bast Fiber Pulps

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