Xylanase production by a novel halophilic bacterium increased 20-fold by response surface methodology

Optimization of selective production media for enhanced production of xylanases in submerged fermentation by Thielaviopsis basicola MTCC 1467 using L16 orthogonal array

“…Enzyme production Xylanase production was studied at 30°C for 10 days in production medium (100 ml) containing rice straw 2 g, MgSO 4 0.1 g, KH 2 PO 4 0.2 g, peptone 1 g, and (Wejse et al 2003). The phenomenon of sudden increase and subsequent downfall in enzyme activities during the cultivation period has also been observed in xylanase production by Aspergillus sydowii MG-49 (Ghosh et al 1993) and Streptomyces sp.…”

“…Production of maximum extracellular chitin deacetylase (460.4±14.7 unit/g initial dry substrate) was obtained by Colletotrichum lindemuthianum ATCC 56676 under solid substrate fermentation (Suresh et al 2011). Hence, use of cost-effective substrates such as lignocellulosic waste material is highly recommended (Wejse et al 2003;Sá-Pereira et al 2002).…”

Enhanced production of histamine dehydrogenase by <i>Natrinema gari</i> BCC 24369 in a non-sterile condition

“…4b). Halophilic archaea have a wide range of temperature preferences depending upon the nature of adaptation and salt requirements (Wejse et al, 2003). Most halophilic archaea and their enzyme production have rather high temperature optima, in the range between 35 and 50°C (Shand and Perez, 1999;Vidyasagar et al, 2006).…”

“…Due to its low solubility in salt-saturated brines, oxygen may easily become a limiting factor of the development of halophilic archaea (Oren, 2006). Wejse et al (2003) also investigated that an increase in the oxygen transfer rate from 130 to 170 rpm resulted in an approximately 10% increase in xylanase production by halophilic eubacterium, strain SX15.…”

“…Among various medium components, NaCl and organic components including complex nitrogen and carbon sources were the key factors having an extreme influence on the growth of cells and the enzyme production of halophilic archaea (Manikandan et al, 2009;Vidyasagar et al, 2006;Wejse et al, 2003). The commonly used organic components included casamino acid, yeast extract and tryptone (Oren, 2006).…”