Xylanase and β-glucanase improve growth performance, gut barrier, and microbiota of pre-growout Nile tilapia, Oreochromis niloticus fed a vegetable-based diet

Brito, Johnny Martins de; Urbich, Allan Vinnícius; Cruz, Thaís Pereira da; Panczevicz, Paola Aparecida Paulovski; Miranda, João Antônio Galiotto; Wernick, Bruno; Furuya, Valéria Rossetto Barriviera; Furuya, Wilson Massamitu

doi:10.1016/j.aquaculture.2022.738653

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…Dubosiella was considered a potential probiotic because it is positively correlated with butyric acid levels [ 87 ]. Bifidobacterium is butyrate-producing bacteria, and the increased short-chain fatty acids were crucial to the improvement of immunity in hosts [ 88 – 91 ]. In the present study, diet FF also increased the abundance of pathogenic bacterium Lachnoclostridium , Romboutsia , Chryseobacterium , and Aeromonas .…”

Section: Discussionmentioning

confidence: 99%

Fishmeal Protein Replacement by Defatted and Full-Fat Black Soldier Fly Larvae Meal in Juvenile Turbot Diet: Effects on the Growth Performance and Intestinal Microbiota

Zhao

Pan

Zhang

et al. 2023

Aquaculture Nutrition

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A 12-week feeding trial was conducted to investigate the effect of the same fishmeal protein level replaced by black soldier fly larvae (Hermetia illucens) meal (BSFL) with different lipid contents on the growth performance and intestinal health of juvenile turbot (Scophthalmus maximus L.) (initial body weight 12.64 g). Three isonitrogenous and isolipidic diets were formulated: fish meal-based diet (FM), diets DF and FF, in which 14% fish meal protein of the FM diet was replaced by defatted and full-fat BSFL, respectively. There were no significant differences in growth performance, intestinal morphology, and mucosal barrier function between the DF and the FM group. However, diet FF markedly reduced the growth performance, intestinal perimeter ratio, and the gene expression of anti-inflammatory cytokine TGF-β ( P < 0.05 ). Compared to group FF, the communities of intestinal microbiota in group DF were more similar to group FM. Moreover, diet DF decreased the abundance of some potential pathogenic bacteria and enriched the potential probiotics, such as Bacillus. Diet FF obviously altered the composition of intestinal microbiota and increased the abundance of some potential pathogenic bacteria. These results suggested that the application of defatted BSFL showed more positive effects on fish growth and intestinal health than the full-fat BSFL, and the intestinal microbiota was closely involved in these effects.

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Section: Discussionmentioning

confidence: 99%

Fishmeal Protein Replacement by Defatted and Full-Fat Black Soldier Fly Larvae Meal in Juvenile Turbot Diet: Effects on the Growth Performance and Intestinal Microbiota

Zhao

Pan

Zhang

et al. 2023

Aquaculture Nutrition

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“…Previous work with supplementing a multi-carbohydrase enzyme mixture to broiler chicken diets (0.05%) shows improvements in the digestibility of dry matter, crude protein, and energy and increases in growth performance [ 41 ]. With Nile Tilapia ( Oreochromis niloticus ), supplementation of a Xylanase and β-glucanase blend (0.20 g/kg of diet) to a vegetable-based diet showed improvements in growth performance, gut morphology, and gut health [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Enrichment of a Plant Feedstuff Mixture’s Nutritional Value through Solid-State Fermentation

Filipe,

Vieira,

Ferreira

et al. 2023

Animals

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Plant feedstuffs are the main ingredients of animal feed. Owing to food–feed competition, increasing the utilization efficiency of these feedstuffs is important for animal nutrition. This can be achieved via solid-state fermentation (SSF). SSF of a plant feedstuff mixture (PFM) (25% rapeseed meal, soybean meal, rice bran, and sunflower meal) by three fungi (Aspergillus ibericus MUM 03.29, Aspergillus niger CECT 2088, and Aspergillus niger CECT 2915) resulted in an increase in protein content by 5%, irrespective of fungi, a reduction in cellulose content by 9 to 11%, and of hemicellulose content by 21 to 34%, relative to unfermented PFM. Enzyme production was measured: the highest cellulase (123.7 U/g), xylanase (431.8 U/g), and beta-glucosidase (117.9 U/g) activity were achieved with A. niger CECT 2088. Principal component analysis showed a positive correlation between all fermented PFMs and enzyme production, protein content, digestibility, and fiber reduction. Bioprocessing of the PFM by SSF increased its nutritional value and digestibility, making it more appealing for animal feeds.

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“…The xylanase activity in feeds was measured by quantifing reducing sugar equipvalent (xylose or glucose) after incubation in 0.05 mol/L sodium acetate buffer (pH 5.3) at 50 °C for 60 min and used colorimetic assay at 540 nm following Inborr et al [43] and Moita et al [13]. Dietary β-glucanase activity was measured using a β-glucanase commercial assay kit (Megazyme, Ireland) with modifications in the amounts of samples following Duarte et al [20] and de Brito et al [44].…”

Section: Chemical Analysismentioning

confidence: 99%

Investigation of the nutritional and functional roles of a combinational use of xylanase and β-glucanase on intestinal health and growth of nursery pigs

Choi,

Duarte,

Pasquali

et al. 2024

J Animal Sci Biotechnol

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Background Xylanase and β-glucanase combination (XG) hydrolyzes soluble non-starch polysaccharides that are anti-nutritional compounds. This study aimed to evaluate the effects of increasing levels of XG on intestinal health and growth performance of nursery pigs. Methods Forty pigs (6.5 ± 0.4 kg) were assigned to 5 dietary treatments and fed for 35 d in 3 phases (11, 9, and 15 d, respectively). Basal diets mainly included corn, soybean meal, and corn distiller’s dried grains with solubles, contained phytase (750 FTU/kg), and were supplemented with 5 levels of XG at (1) 0, (2) 280 TXU/kg xylanase and 125 TGU/kg β-glucanase, (3) 560 and 250, (4) 840 and 375, or (5) 1,120 and 500, respectively. Growth performance was measured. On d 35, all pigs were euthanized and jejunal mucosa, jejunal digesta, jejunal tissues, and ileal digesta were collected to determine the effects of increasing XG levels and XG intake on intestinal health. Results Increasing XG intake tended to quadratically decrease (P = 0.059) viscosity of jejunal digesta (min: 1.74 mPa·s at 751/335 (TXU/TGU)/kg). Increasing levels of XG quadratically decreased (P < 0.05) Prevotellaceae (min: 0.6% at 630/281 (TXU/TGU)/kg) in the jejunal mucosa. Increasing XG intake quadratically increased (P < 0.05) Lactobacillaceae (max: 40.3% at 608/271 (TXU/TGU)/kg) in the jejunal mucosa. Increasing XG intake quadratically decreased (P < 0.05) Helicobacteraceae (min: 1.6% at 560/250 (TXU/TGU)/kg) in the jejunal mucosa. Increasing levels of XG tended to linearly decrease (P = 0.073) jejunal IgG and tended to quadratically increase (P = 0.085) jejunal villus height to crypt depth ratio (max: 2.62 at 560/250 (TXU/TGU)/kg). Increasing XG intake tended to linearly increase the apparent ileal digestibility of dry matter (P = 0.087) and ether extract (P = 0.065). Increasing XG intake linearly increased (P < 0.05) average daily gain. Conclusions A combinational use of xylanase and β-glucanase would hydrolyze the non-starch polysaccharides fractions, positively modulating the jejunal mucosa-associated microbiota. Increased intake of these enzyme combination possibly reduced digesta viscosity and humoral immune response in the jejunum resulting in improved intestinal structure, and ileal digestibility of nutrients, and finally improving growth of nursery pigs. The beneficial effects were maximized at a combination of 550 to 800 TXU/kg xylanase and 250 to 360 TGU/kg β-glucanase.

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Xylanase and β-glucanase improve growth performance, gut barrier, and microbiota of pre-growout Nile tilapia, Oreochromis niloticus fed a vegetable-based diet

Cited by 6 publications

References 55 publications

Fishmeal Protein Replacement by Defatted and Full-Fat Black Soldier Fly Larvae Meal in Juvenile Turbot Diet: Effects on the Growth Performance and Intestinal Microbiota

Fishmeal Protein Replacement by Defatted and Full-Fat Black Soldier Fly Larvae Meal in Juvenile Turbot Diet: Effects on the Growth Performance and Intestinal Microbiota

Enrichment of a Plant Feedstuff Mixture’s Nutritional Value through Solid-State Fermentation

Investigation of the nutritional and functional roles of a combinational use of xylanase and β-glucanase on intestinal health and growth of nursery pigs

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