Xylanase and cellulase of fungus Cerrena unicolor VKM F-3196: Production, properties, and applications for the saccharification of plant material

Belova, O. V.; Lisov, A. V.; Vinokurova, N. G.; Костеневич, А. А.; Сапунова, Л. И.; Lobanok, A. G.; Leontievsky, A. A.

doi:10.1134/s0003683814020057

Cited by 19 publications

(15 citation statements)

References 28 publications

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“…Many lignin-containing agricultural byproducts can stimulate white rotting fungi to produce biotechnologically applicable ligninolytic and hydrolytic enzymes (Żuchowski et al, 2013;Belova et al, 2014). The results obtained clearly indicate that SLF of the RM-containing medium is a very good growth environment for C. unicolor.…”

Section: Resultsmentioning

confidence: 81%

“…C. unicolor has been reported to produce cellulases and xylanase, especially when it is grown in a medium containing microcrystalline cellulose (Belova et al, 2014;Elisashvili et al, 2002). The submerged fermentation of rapeseed meal by C. unicolor was correlated with a very distinct increase in β-glucosidase activities in the medium and in mycelia (Fig 3A, C).…”

Section: β-Glu Activitiesmentioning

confidence: 91%

“…The biotechnological application of both groups of enzymes has been broadly investigated for many years. C. unicolor is a white rot fungus belonging to the phylum Basidiomycetes, known as a very efficient laccase or hydrolase producer (Janusz et al, 2007;Belova et al, 2014). It can also be used to produce dietary nutraceuticals or supplements having antioxidant and antibacterial properties .…”

Section: Introductionmentioning

confidence: 99%

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Effective and complex stimulation of the biodegradation system of fungus Cerrena unicolor by rapeseed meal fermentation

Jaszek¹,

Miłek²,

Żuchowski³

et al. 2016

Acta Biochim Pol

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The effect of supplementation of medium with rapeseed meal (RM) on production of biotechnologically important enzymes was investigated in submerged cultures of the white rot fungus Cerrena unicolor. The addition of RM (3.5% w/v) distinctly stimulated the activities of laccase, chitinase, and β-glucosidase. As compared to the control, the activities of chitinase, β-glucosidase, and laccase in the RM supplemented cultures were up to 4.1, 8.4, and 3.9 times higher, respectively. The results of the spectrophotometric and spectrofluorometric measurements were additionally confirmed by zymographic analysis of the samples. The level of sugars and phenolic compounds as well as the antioxidative ability of fungal preparations were also determined. The results obtained indicate that the submerged liquid fermentation of rapeseed meal can be proposed as an inexpensive and very effective method for biotechnological production of chitinase, β-glucosidase, and laccase by C. unicolor.

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Section: Resultsmentioning

confidence: 81%

Section: β-Glu Activitiesmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Effective and complex stimulation of the biodegradation system of fungus Cerrena unicolor by rapeseed meal fermentation

Jaszek¹,

Miłek²,

Żuchowski³

et al. 2016

Acta Biochim Pol

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“…Cerrena unicolor, a mossy maze polypore, is a globally distributed wood-degrading basidiomycete that causes extensive white rot (Enebak & Blanchette, 1989;Roody, 2003). C. unicolor strains are also well described as potential bioproducers of industrially relevant enzymes and other bioactive compounds of medical importance (Janusz et al, 2007;Hibi et al, 2012;Belova et al, 2014;Mizerska-Dudka et al, 2015;Sulej et al, 2015). A recent RNAseq-based transcriptomic analysis has provided new Vol.…”

Section: Introductionmentioning

confidence: 99%

Light-regulated synthesis of extra- and intracellular enzymes related to wood degradation by the white rot fungus Cerrena unicolor during solid-state fermentation on ash sawdust-based medium

et al. 2019

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The light-dependent metabolism of the white rot basidiomycete Cerrena unicolor FCL139 has already been demonstrated using transcriptomic and Biolog-based approaches. To further analyze the influence of light on C. unicolor wood degradation, we measured the activity of an array of CAZymes (carbohydrate-active enzymes) and enzymes involved in the redox system of fungal cells associated with lignolysis. Extra- and intracellular enzymatic extracts were obtained from solid-state ash sawdust C. unicolor cultures cultivated for 14 days under red, blue, green, or white light conditions, or in the dark. Light greatly influenced the synthesis of MnP, total cellulases, endo-1,4-β-glucanase, endo-1,4-β-xylanase, catalase, and superoxide dismutase. The production of MnP and catalase was evidently stimulated by white light. It is also worth noticing that blue light caused a gradual increase in the activity of total cellulases throughout the entire period of C. unicolor growth. Moreover, endo-1,4-β-glucanase showed the highest activity on day 13 of fungus cultivation and the production of laccase and β-glucosidase appeared to be the least influenced by light. However, the strongest activity of the endo-1,4-β-xylanase was observed in the dark. It seemed that light not only influenced the regulation of the synthesis of the wood-degrading enzymes at different levels, but also acted indirectly by affecting production of enzymes managing harmful lignin by-products causing oxidative stress. The ability of the fungus to decompose woody plant material is clearly influenced by environmental factors.

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“…Among the many hitherto-characterized fungal species, Cerrena unicolor was described in literature as one of the best laccase producers [ 5 ]. Moreover, this species belonging to Aphyllophorales was proved to secrete extracellular manganese peroxidase, versatile peroxidases [ 6 ], and xylanase or cellulase when grown on cellulose [ 7 ]. This fungus commonly called “mossy maze polypore” may be found on dead northern hardwood tree species as maple, birch, or alder, where it causes white rot [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Cellobiose Dehydrogenase from a Biotechnologically Important Cerrena unicolor Strain

Sulej

Janusz

Osińska-Jaroszuk

et al. 2015

Appl Biochem Biotechnol

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Cellobiose dehydrogenase (CDH), a secreted flavocytochrome produced by a number of wood-degrading fungi, was detected in the culture supernatant of a biotechnologically important strain of Cerrena unicolor grown in a modified cellulose-based liquid medium. The enzyme was purified as two active fractions: CuCDH-FAD (flavin domain) (1.51-fold) with recovery of 8.35 % and CuCDH (flavo-heme enzyme) (21.21-fold) with recovery of 73.41 %. As CDH from other wood-rotting fungi, the intact form of cellobiose dehydrogenase of C. unicolor is a monomeric protein containing one flavin and one heme b with molecular mass 97 kDa and pI = 4.55. The enzyme is glycosylated (8.2 %) mainly with mannose and glucosamine residues. Moreover, the cellobiose dehydrogenase gene cdh1 and its corresponding cDNA from the fungus C. unicolor were isolated, cloned, and characterized. The 2316-bp full-length cDNA of cdh1 encoded a mature CDH protein containing 771 amino acids preceded by a signal peptide consisting of 18 amino acids. Moreover, both active fractions were characterized in terms of kinetics, temperature and pH optima, and antioxidant properties.

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Xylanase and cellulase of fungus Cerrena unicolor VKM F-3196: Production, properties, and applications for the saccharification of plant material

Cited by 19 publications

References 28 publications

Effective and complex stimulation of the biodegradation system of fungus Cerrena unicolor by rapeseed meal fermentation

Effective and complex stimulation of the biodegradation system of fungus Cerrena unicolor by rapeseed meal fermentation

Light-regulated synthesis of extra- and intracellular enzymes related to wood degradation by the white rot fungus Cerrena unicolor during solid-state fermentation on ash sawdust-based medium

Characterization of Cellobiose Dehydrogenase from a Biotechnologically Important Cerrena unicolor Strain

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