Xy99-5038 Promotes Long-Term Survival of Cultured Retinal Neurons

Abstract

We have previously reported that XY99-5038, a preparation from a specific formula of Traditional Chinese Medicine, could effectively inhibit hydrogen peroxide-induced retinal cell death. In the present study, we investigated the possibility of XY99-5038 to prolong neuronal survival in a long-term retinal neuronal culture. Basic fibroblast survival factor (bFGF), a potent neurotrophic factor, was employed as comparable agent. Retinas of 0-2 days old Sprague-Dawley rats were isolated and dissociated. The cells were maintained in tissue culture for up to 9 weeks in a synthetic serum-free media. XY99-5038 (100 ng/ml) or a vehicle was added to culture every 3-4 days, starting at the first week of culturing. The number of cells were counted and compared for each time point and treatment. Cell viability was also determined by MTT assay, whereas apoptotic cell death was evaluated by the TUNEL assay. XY99-5038 treatment significantly reduced cell loss, increased cell viability, and inhibited apoptosis in this long-term retinal neuronal culture. Our data also show that the protective effect of XY99-5038 is more potent than that of bFGF. Our data suggest that XY99-5038 could be beneficial to the prolongation of neuron survival.

External Qi of Yan Xin Qigong induces cell death and gene expression alterations promoting apoptosis and inhibiting proliferation, migration and glucose metabolism in small-cell lung cancer cells

“…Long-term clinical observations and ongoing studies have shown that patients with cancer and other medical conditions received significant beneficial effects from exposure to External Qi of Yan Xin Qigong (YXQ-EQ) without noticeable side effects [8, 9]. A body of substantial experimental studies has been conducted to show the effects of YXQ-EQ at molecular, cellular, and microorganism levels [7, 10–14]. Most recently, it was reported that YXQ-EQ exhibited potent cytotoxic effects on BxPC3 pancreatic cancer cells [4], PC3 prostate cancer cells [5] and MDA-MB-231 breast cancer cells [6], respectively, by effectively inhibiting ERK1/2 and/or Akt pathways in these cancer cells, without harming normal cells.…”

External Qi of Yan Xin Qigong Inhibits Activation of Akt, Erk/12 and NF-ĸB and Induces Cell Cycle Arrest and Apoptosis in Colorectal Cancer Cells

“…No cytotoxicity to normal cells was observed in these studies with YXQ-EQ. In fact, in vitro studies have found that YXQ-EQ was capable of protecting neurons from oxidative stress induced apoptosis and significantly prolonged survival of neurons [20,21]. …”

王鳳儀倫理療病闡析——儒家生命倫理之活例

“…(3) 90 "Protective Effect of XY99-5038 on Hydrogen Peroxide Induced Cell Death in Cultured Retinal Neurons" (Yan et al 2001, 289-299), "XY99-5038 Promotes Long-Term Survival of Cultured Retinal Neurons" ( Yan et al 2002( Yan et al , 1209( Yan et al -1227 " 2014 , 1970).〕…”