1952
DOI: 10.1111/j.1365-2818.1952.tb02336.x
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Xviii.—polyethylene Glycols as Histological Embedding Media: With a Note on the Dimensional Change of Tissue During Embedding in Various Media

Abstract: Synopsis The literature is reviewed. A technique for embedding tissues in polyethylene glycol 1,000 mono‐stearate (Nonex 63B) after preliminary infiltration with polyethylene glycol 900 is described. Preliminary dehydration in alcohol is not required and at no stage is the temperature of the tissue raised above 40° C. Measurement of tissue before and after embedding in Nonex, paraffin and celloidin shows that there is significantly less shrinkage in Nonex. It is shown that the celloidin in celloidin‐Nonex mixt… Show more

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Cited by 32 publications
(5 citation statements)
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“…However, it seems that the degree of shrinkage of paraffin-embedded tissue is not caused by compression of the tissue by the contracting wax, but rather by the prolonged high temperature of the wax oven [9,10]. Our figures of about 6% shrinkage after embedding in 56-57~ Paraplast correspond to similar figures quoted by Miles and Linder [2] also for cancellous bone, as compared to the shrinkage of the paraffin-embedded compact bone, which they quote to be about 4%. Because of this obvious and constant reduction in size after paraffin wax embedding, many authors recommend embedding tissue in cellulose nitrate (celloidin or low-viscosity nitrocellulose [LVN]) [11] or in polyethylene glycol [2].…”
Section: Discussionsupporting
confidence: 91%
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“…However, it seems that the degree of shrinkage of paraffin-embedded tissue is not caused by compression of the tissue by the contracting wax, but rather by the prolonged high temperature of the wax oven [9,10]. Our figures of about 6% shrinkage after embedding in 56-57~ Paraplast correspond to similar figures quoted by Miles and Linder [2] also for cancellous bone, as compared to the shrinkage of the paraffin-embedded compact bone, which they quote to be about 4%. Because of this obvious and constant reduction in size after paraffin wax embedding, many authors recommend embedding tissue in cellulose nitrate (celloidin or low-viscosity nitrocellulose [LVN]) [11] or in polyethylene glycol [2].…”
Section: Discussionsupporting
confidence: 91%
“…Patten and Philpott [1] recorded shrinkage of tissue in pig embryos in a variety of fixatives and at different stages of paraffin wax embedding. Miles and Linder [2] studied the influence of various embedding media on dimensional changes in different tissues including cancellous bone, and A. Smith [3] investigated the effect of drying on tissue sections after cutting and also changes in their stainability due to adherence to the microscopic slide. Brain [4] studied and measured quantitative changes during the dehydration and clearing processes in several agents and also while cutting a decalcified head of a mouse fetus.…”
Section: Summary Distortion Of the Dimensions Of Largementioning
confidence: 99%
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“…If the test material must be retained in the tissue sections, then cryopreservation and cryomicrotomy may be considered. Direct infiltration using hydrophilic embedding media such as glycolmethacrylate or PEG may also be considered (Miles and Linder 1952). However, care must be taken to avoid exposing the microscopic tissue preparation to reagents that dissolve the implant material during slide staining.…”
Section: Issues With Bioresorbable Devicesmentioning
confidence: 99%
“…Cell Dimensions.-Auricles were cooled or were poisoned with ouabain until they ceased to beat and were then fixed in saline containing 10% (v/v) of neutralized formalin, and embedded in polyethyleneglycol-1000 monostearate (Miles and Linder, 1952). Sections were stained with haematoxylin and van Gieson, magnified x 1,000 and projected on a screen so that the diameter of the cells could be measured.…”
Section: Methodsmentioning
confidence: 99%