The extent of inner ear membrane distention and rupture in the ears of a patient with otosclerosis, who had an oval window fistula 6 years after stapedectomy, was demonstrated by immunohistochemical staining of the keratin in the celIoidinembedded temporal bone sections according to an antigenretrieval technique. 1 The ability to demonstrate clearly the position of, and define the edges of, inner ear membranes derived from ectoderm, with a keratin ectoderm-specific stain, was instrumental in developing a hypothesis to explain the symptoms resulting from inner ear membrane raptures in this patient.
CASE REPORTThe temporal bones reported in this study were from a man first examined in 1957, at the age of 54 years. He had a progressiwe bilateral hearing loss with an average of 88 dB for speech frequencies in the right ear and 71 dB in the left ear. Physical examination of the ears revealed thin and intact tympanic membranes. Audiometric tests, with masking, elicited bilateral mixed hearing losses (Fig. 1A). Bone conduction was better than air conduction with the tuning fork test at t024 Hz, bilaterally.A diagnosis of otosclerosis was made and the patient underwent a right stapes mobilization and subsequent revision stapedectomies with a polyethelene strut and then a vein plug. At the last surgery it was found that the round window was closed by otosclerosis, so the prognosis was guarded. However, there was an improvement to a level of 60 DB (converted from the American Standards Association) in the speech frequencies recorded in 1960. There was a gradual decline in air and bone conduction until the last test in 1977, 7 years before the patient's death (Fig. 1B).In spite of the expected poor result from surgery on the right ear because of the sensorineural component of the loss, it became the better-hearing ear after surgery, so the left ear underwent a stapedectomy with a polyethelene strut over Gelfoam with a resultant improvement in hearing to 40 dB.Six years after the last surgery, during an upper respirato-From the