XPS study of protein adsorption onto nanocrystalline aluminosilicate microparticles

Vanea, E.; Simon, V.

doi:10.1016/j.apsusc.2010.09.101

Cited by 96 publications

(75 citation statements)

References 39 publications

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“…The peaks situated at lower binding energy (ie, ~398.9 eV and 399.9-400.7 eV) were associated with neutral amine and carbon-bonded nitrogen in an amidic bond, respectively. [64][65][66][67][68][69][70][71][72] The peak situated at higher binding energy (~400.3-402.2 eV) pertained to the protonated amine groups. In the case of samples tested in DS (which contained only amino acids), the spectra exhibited only the maxima of amino groups: protonated amino groups at higher binding energy (~400.3 eV), and unprotonated (neutral) amino groups at lower binding energies (~398.9 eV) ( Figure 15A).…”

Section: Discussionmentioning

confidence: 99%

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Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

Popa¹,

Stan²,

Husanu³

et al. 2017

IJN

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Synthetic physiological fluids are currently used as a first in vitro bioactivity assessment for bone grafts. Our understanding about the interactions taking place at the fluid–implant interface has evolved remarkably during the last decade, and does not comply with the traditional International Organization for Standardization/final draft International Standard 23317 protocol in purely inorganic simulated body fluid. The advances in our knowledge point to the need of a true paradigm shift toward testing physiological fluids with enhanced biomimicry and a better understanding of the materials’ structure-dissolution behavior. This will contribute to “upgrade” our vision of entire cascades of events taking place at the implant surfaces upon immersion in the testing media or after implantation. Starting from an osteoinductive bioglass composition with the ability to alleviate the oxidative stress, thin bioglass films with different degrees of polymerization were deposited onto titanium substrates. Their biomineralization activity in simulated body fluid and in a series of new inorganic–organic media with increasing biomimicry that more closely simulated the human intercellular environment was compared. A comprehensive range of advanced characterization tools (scanning electron microscopy; grazing-incidence X-ray diffraction; Fourier-transform infrared, micro-Raman, energy-dispersive, X-ray photoelectron, and surface-enhanced laser desorption/ionization time-of-flight mass spectroscopies; and cytocompatibility assays using mesenchymal stem cells) were used. The information gathered is very useful to biologists, biophysicists, clinicians, and material scientists with special interest in teaching and research. By combining all the analyses, we propose herein a step forward toward establishing an improved unified protocol for testing the bioactivity of implant materials.

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Section: Discussionmentioning

confidence: 99%

“…65,68,69 When amino acids and proteins are present in the environment (DS, DC, DCC), a new component appears at ~285.9-286.1 eV, associated with C-N bonds. 64,66,67,[70][71][72] Its position is rather robust, irrespective of changes in the testing conditions (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

Popa¹,

Stan²,

Husanu³

et al. 2017

IJN

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“…Usually the protein attachment on surfaces involves the evaluation of nitrogen and carbon, oxygen and nitrogen as key elements for protein identification [13].…”

Section: Surface Coverage Explored By Xpsmentioning

confidence: 99%

“…By adding radioactivable isotopes like yttrium, dysprosium, samarium, or rhenium, these materials can be used for in situ radiotherapy, while the addition of iron oxide makes them suitable for hyperthermia applications [11]. The simultaneous application of both radiotherapy and hyperthermia considerably enhances the therapeutic effects of these cancer treatment methods [12,13]. In both therapy cases, the aluminosilicate particles have to be injected locally or intravenously at the tumor site, and they are rapidly coated by components of circulation, such as blood plasma proteins.…”

Section: Introductionmentioning

confidence: 99%

Glass-Ceramics: Fundamental Aspects Regarding the Interaction with Proteins

Gruian¹,

Vanea²,

Steinhoff³

et al. 2015

Handbook of Bioceramics and Biocomposites

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“…After incubation, the films were washed in distilled water for 20 min, first soaking and then rinsing 3 times. The films were dried in a new plate and left at room temperature for 12 h. Some films were analyzed for the chemical structure using x-ray photoelectron spectroscopy (XPS) (Shimadzu, Japan) [19,20]. Survey spectra of XPS were acquired from 0-1200 eV, with a pass energy of 80 eV and step size of 1 eV.…”

Section: Protein Adsorption Analysismentioning

confidence: 99%

Wettability Effect of PECVD-SiO_xFilms on Poly(lactic acid) Induced by Oxygen Plasma on Protein Adsorption and Cell Attachment

Sarapirom

Lee

Jin

et al. 2013

J. Phys.: Conf. Ser.

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Abstract. Surface wettability is an important property of biomaterials. Silicon oxide films have a wide range of applications due to a range of the properties such as the mechanical strength and surface wettability. This paper reports effect of the surface wettability of silicon oxide (SiO x ) films on protein adsorption and cell attachment and proliferation. SiO x films were deposited onto poly(lactic acid) (PLA) substrate using plasma enhanced chemical vapor deposition (PECVD). Octamethylcyclotetrasiloxane (OMCTS:Si 4 O 4 C 8 H 24 ) was used as a precursor with O 2 as a carrier gas. After deposition, the films were treated with O 2 -plasma to adapt wettability. It was found that O 2 -plasma enhanced the wettability of the films without changing the film thickness, while made the surface morphology slightly smoother. The polar component increased after O 2 -plasma treatment as observed in the contact angle measurements. The surface energy of the films was calculated by means of the Owens-Wendt method to resolve the contributions of polar and dispersive components. The chemical structure was characterized using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. The films were dense with a high Si-network structure. The reduced carbon content (-CH n , Si-CH 3 ) and increased hydrogen content (-OH) of the O 2 -plasma treated SiO x films led to the polar components enhancing the SiO x wettability. Adsorption of bovine serum albumin (BSA) on the films was investigated by using x-ray photoelectron spectroscopy (XPS). More BSA was adsorbed onto the O 2 -plasma treated SiO x films. Attachment and proliferation of MC3T3-E1 mouse pre-osteoblasts and L929 mouse fibroblasts cells on the SiO x films were evaluated via MTT assay. The cells were attached more to the untreated SiO x films but proliferated more on the surface of the O 2 -plasma treated SiO x films depending on the cell types.

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XPS study of protein adsorption onto nanocrystalline aluminosilicate microparticles

Cited by 96 publications

References 39 publications

Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

Glass-Ceramics: Fundamental Aspects Regarding the Interaction with Proteins

Wettability Effect of PECVD-SiO_xFilms on Poly(lactic acid) Induced by Oxygen Plasma on Protein Adsorption and Cell Attachment

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XPS study of protein adsorption onto nanocrystalline aluminosilicate microparticles

Cited by 96 publications

References 39 publications

Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

Bioglass implant-coating interactions in synthetic physiological fluids with varying degrees of biomimicry

Glass-Ceramics: Fundamental Aspects Regarding the Interaction with Proteins

Wettability Effect of PECVD-SiOxFilms on Poly(lactic acid) Induced by Oxygen Plasma on Protein Adsorption and Cell Attachment

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Wettability Effect of PECVD-SiO_xFilms on Poly(lactic acid) Induced by Oxygen Plasma on Protein Adsorption and Cell Attachment