Xpert MTB/RIF Ultra assay for the detection of Mycobacterium tuberculosis in people with negative conventional Xpert MTB/RIF but chest imaging suggestive of tuberculosis in Dhaka, Bangladesh

Biswas, Shwapon; Uddin, Mohammad Khaja Mafij; Paul, Kishor Kumar; Ather, Md. Fahim; Ahmed, Shahriar; Nasrin, Rumana; Kabir, Shahjahan; Heysell, Scott K

doi:10.1016/j.ijid.2021.11.010

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…These outcomes strongly encourage using "trace" as a positive result for diagnostics of tuberculous pleurisy ( Gao et al , 2021 ). In addition, Biswas et al demonstrated that 8 of 16 Xpert-Ultra "trace" sputum retests with Xpert-Ultra turned categorically positive ( Biswas et al , 2022 ). We confronted many trace outcomes by Xpert-Ultra in this study as well.…”

Section: Discussionmentioning

confidence: 74%

Xpert MTB/RIF Ultra outperformed the Xpert assay in tuberculosis lymphadenitis diagnosis: a prospective head-to-head cohort study

Zhang

Kong

et al. 2022

International Journal of Infectious Diseases

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Section: Discussionmentioning

confidence: 74%

Xpert MTB/RIF Ultra outperformed the Xpert assay in tuberculosis lymphadenitis diagnosis: a prospective head-to-head cohort study

Zhang

Kong

et al. 2022

International Journal of Infectious Diseases

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“…Because Ultra can detect MTB at “trace” levels, this feature can be helpful in establishing TB diagnoses in paucibacillary conditions. Studies have shown that the “trace” calls result in the early diagnosis of TB in smear-negative, culture-positive paucibacillary situations, leading to prompt treatments [ 25 , 26 ]. This highlights the significant clinical impact of Ultra’s “trace” feature in critical situations where the diagnosis could have been missed or delayed otherwise.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Analytical Sensitivity (Limit of Detection) of Xpert MTB/RIF and Xpert MTB/RIF Ultra for Non-Sputum Specimens

et al. 2023

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Tuberculosis (TB) is a significant public health threat and has remained a leading cause of death in many parts of the world. Rapid and accurate testing and timely diagnosis can improve treatment efficacy and reduce new exposures. The Cepheid Xpert® MTB/RIF tests have two marketed products (US-IVD and Ultra) that are widely accepted for diagnosis of TB but have not yet been approved for non-sputum specimens. Despite numerous studies in the literature, no data for the analytical sensitivity of these two products on the non-sputum samples are available to date. This is the first study that systematically determined the analytical sensitivities of both US-IVD and Ultra tests on cerebrospinal fluid (CSF), tissue, and bronchoalveolar lavage (BAL). The limits of detection (LoDs) on the US-IVD test for both Mycobacterium tuberculosis and rifampin resistance in CFU/mL, respectively, were as follows: CSF (3.3 and 4.6), tissue (15 and 23), and bronchoalveolar lavage (BAL) (45 and 60), and on the Ultra test: CSF (0.16 and 2.7), tissue (0.11 and 12), and BAL (0.65, and 7.5). Overall, the analytical sensitivities of the Ultra test were substantially better than US-IVD for all sample types tested. This study provided a foundation for using either the US-IVD or Ultra test for the early detection of both pulmonary and extrapulmonary (EP) TB. Furthermore, using Ultra could result in higher TB case detection rates in subjects with paucibacillary TB and EP TB, positively impacting WHO goals to eradicate TB.

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“…This suggests that the Xpert® MTB/RIF Ultra tool can effectively serve in TB screening and treatment. 27,28 The heightened sensitivity of the device contributes significantly to increased TB detection, providing a more reliable identification of rifampicin resistance. 29 This is particularly noteworthy as MTB can still be detected even in cases with negative culture test results due to the presence of MTB DNA in the examined samples.…”

Section: Sensitivity Values Of the Afb And Xpert® Mtb/rif Ultra Tests...mentioning

confidence: 99%

Concordance of Sputum and Feces Samples for Detecting Mycobacterium Tuberculosis using Xpert® MTB/RIF Ultra

Rochmawati,

Wardhani,

Puspitasari

et al. 2024

Pharmacogn J.

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Xpert MTB/RIF Ultra assay for the detection of Mycobacterium tuberculosis in people with negative conventional Xpert MTB/RIF but chest imaging suggestive of tuberculosis in Dhaka, Bangladesh

Cited by 11 publications

References 27 publications

Xpert MTB/RIF Ultra outperformed the Xpert assay in tuberculosis lymphadenitis diagnosis: a prospective head-to-head cohort study

Xpert MTB/RIF Ultra outperformed the Xpert assay in tuberculosis lymphadenitis diagnosis: a prospective head-to-head cohort study

Comparison of Analytical Sensitivity (Limit of Detection) of Xpert MTB/RIF and Xpert MTB/RIF Ultra for Non-Sputum Specimens

Concordance of Sputum and Feces Samples for Detecting Mycobacterium Tuberculosis using Xpert® MTB/RIF Ultra

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