XPD and hOGG1 gene polymorphisms in reperfusion oxidative stress

Isbir, O.; Görmüş, Uzay; Ergen, Arzu; Çakmakoğlu, Bedia; Kahraman, Özlem Tımırcı; Baykan, Nigar

doi:10.4238/2011.december.19.4

Cited by 3 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…( 11 ). Our results regarding the frequencies of studied gene polymorphisms are consistent with those of previous Turkish studies ( 27 , 28 ). However, our results did not show any statistical difference between CAD patients and controls in terms of GSTM1, GSTT1, and hOGG1 variant allele frequency.…”

Section: Discussionsupporting

confidence: 93%

The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk

et al. 2016

View full text Add to dashboard Cite

Objective:Atherosclerotic coronary artery disease (CAD) appears to be a multifactorial process caused by the interaction of environmental risk factors with multiple predisposing genes. Therefore, in this study we aimed to determine the role of oxidative DNA damage and some variations in glutathione S-transferase (GSTM1 and GSTT1) and DNA repair (hOGG1) genes in CAD risk.Methods:A case-control study was conducted on 59 individuals who had undergone coronary angiographic evaluation. Of these, 29 were patients diagnosed with CAD (mean age =61.5±10.3) and 30 were controls examined for reasons other than suspected CAD and who had angiographically documented normal coronary arteries (mean age =60.4±11.6). Basal DNA damage as well as pyrimidine and purine base damage were evaluated in peripheral blood lymphocytes using the modified comet assay. Polymerase chain reaction-restriction length polymorphism (PCR-RFLP)-based assay was used for genotyping.Results:Basal DNA damage levels in patients [9.16 (3.26)] were significantly higher than those in controls [7.59 (3.23); p=0.017], and basal DNA and pyrimidine base damage levels were significantly correlated with disease severity based on Gensini scoring (r=0.352, p=0.006; r=0.318, p=0.014, respectively). However, no significant differences were observed in terms of oxidized DNA bases between patients and controls. The frequencies of studied genotypes (GSTM1, GSTT1, and hOGG1) were similar between groups.Conclusion:The results of this study pointed out the role of DNA damage in CAD and its severity. However, GSTM1, GSTT1, and hOGG1 gene polymorphisms seemed to have no effect on individual susceptibility for disease progression. (Anatol J Cardiol 2016; 16: 931-8)

show abstract

Section: Discussionsupporting

confidence: 93%

The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In addition, with oxidative stress in reperfusion, the hOGG1 Ser326Cys gene polymorphism was not found related, on the other hand, it was related with the Gln allele of the XPD Lys751Gln polymorphism [24].…”

Section: Discussionmentioning

confidence: 98%

Investigation of DNA repair genes XPD and hOGG1 in type 2 Diabetes mellitus

Yigitbasi¹,

Yurdum²,

Yılmaz³

et al. 2017

Med-Science

View full text Add to dashboard Cite

Increased oxidative stress in type 2 diabetes cause to the accumulation of DNA damage and results diabetic complications. Xeroderma pigmentosum complementation group D (XPD) and human oxoguanine glycosylase 1 (hOGG1) are genes involved in the repair of oxidative DNA damage. In this study, we aimed to evaluate association between XPD Lys751Gln and hOGG1 Ser326Cys polymorphisms with type 2 diabetes mellitus (T2DM) in the Turkish population. Seventy-one T2DM patients and 54 healthy individuals were incorporated into this study. DNA was extracted from whole blood. The Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) techniques were used. There was statistically significant difference between patient and control groups in the genotype distribution of XPD Lys751Gln polymorphism (p˂0.05). While the Lys/Lys genotype was found significantly higher in control group, the Lys/Gln was higher in patients. (p˂0.05). There was no significant difference between groups in the genotype distribution of hOGG1 Ser326Cys polymorphism. Despite the small number of subjects included in the study, it could be interpreted that the Lys/Gln genotype of XPD Lys751Gln polymorphism may be contributing to the development of diabetes.

show abstract