2003
DOI: 10.1074/jbc.m302546200
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Xeroderma Pigmentosum Group C Protein Possesses a High Affinity Binding Site to Human Centrin 2 and Calmodulin

Abstract: Human centrin 2 (HsCen2), a member of the EF-hand superfamily of Ca 2؉ -binding proteins, is commonly associated with centrosome-related structures. The protein is organized in two domains, each containing two EFhand motifs, but only the C-terminal half exhibits Ca

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Cited by 78 publications
(126 citation statements)
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“…Notably, our results from sequence analysis were in agreement with the general predictions deposited in the Pfam-A database (61,62), which also suggest that the region 520-870 is fundamentally distinct from the rest of XPC. The prediction of structure in the region 520-870 is also fully consistent with the mapping of XPC interactions with DNA (6), RAD23B (6), and CETN2 (11,27) to functional domains within XPC-C (Fig. 1C).…”
Section: Analysis Of Xpc Sequencesupporting
confidence: 82%
See 1 more Smart Citation
“…Notably, our results from sequence analysis were in agreement with the general predictions deposited in the Pfam-A database (61,62), which also suggest that the region 520-870 is fundamentally distinct from the rest of XPC. The prediction of structure in the region 520-870 is also fully consistent with the mapping of XPC interactions with DNA (6), RAD23B (6), and CETN2 (11,27) to functional domains within XPC-C (Fig. 1C).…”
Section: Analysis Of Xpc Sequencesupporting
confidence: 82%
“…(C) Schematic diagram of XPC domains and several constructs designed from sequence analyses. The XPC regions proposed to interact with RAD23B (6), TFIIH (5), CETN2 (27), and DNA (6) are highlighted as indicated. Red bars indicate the predicted ordered regions from panel A.…”
Section: Discussionmentioning
confidence: 99%
“…Furthermore our proteinprotein interaction assays show that the assembly of all centrin-transducin complexes is strictly dependent on Ca 2ϩ . Previous biophysical analyses of centrins have indicated that the binding of Ca 2ϩ via their EF-hands induces conformational changes of the molecules resulting in activation of centrins (18,27,48,49). In diverse unicellular green algae, Ca 2ϩ -activated centrins are responsible for the formation and contraction of centrin-containing nanofibers (17,18).…”
Section: Discussionmentioning
confidence: 99%
“…Whether or not centrin-2 is required for centrosome duplication in human cells is controversial, but there is no question that this protein is a genuine component of vertebrate centrosomes [144][145][146][147]. Equally well established is the fact that centrin-2 interacts with xeroderma pigmentosum group C protein, a core component of nucleotide excision repair [148,149]. Much remains to be learned about the functions of centrin-2, but it is remarkable that genetic deletion of all centrin isoforms from chicken DT40 cells resulted in significant delays in nucleotide excision repair, but no obvious phenotypes associated with centrosome duplication or function [144].…”
Section: Centrosomes As Signalling Platforms In Vertebrates? (A) Cellmentioning
confidence: 99%