Xenotransplantation literature update, July–October 2011

Schneider, Mårten K J

doi:10.1111/j.1399-3089.2011.00682.x

Cited by 2 publications

(2 citation statements)

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“…Several approaches (e.g., xenotransplantation) have been proposed to increase the number of potential donor organs. However, previous attempts in humans often resulted in hyperacute rejection and death (22). A much more elegant solution to liver disease is hepatocyte transplantation, and laboratory studies and experiences in small numbers of human subjects have demonstrated its efficacy.…”

Section: Introductionmentioning

confidence: 99%

Human-Scale Whole-Organ Bioengineering for Liver Transplantation: A Regenerative Medicine Approach

et al. 2013

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At this time, the only definitive treatment of hepatic failure is liver transplantation. However, transplantation has been limited by the severely limited supply of human donor livers. Alternatively, a regenerative medicine approach has been recently proposed in rodents that describe the production of three-dimensional whole-organ scaffolds for assembly of engineered complete organs. In the present study, we describe the decellularization of porcine livers to generate liver constructs at a scale that can be clinically relevant. Adult ischemic porcine livers were successfully decellularized using a customized perfusion protocol, the decellularization process preserved the ultrastructural extracellular matrix components, functional characteristics of the native microvascular and the bile drainage network of the liver, and growth factors necessary for angiogenesis and liver regeneration. Furthermore, isolated hepatocytes engrafted and reorganized in the porcine decellularized livers using a human-sized organ culture system. These results provide proof-of-principle for the generation of a human-sized, three-dimensional organ scaffold as a potential structure for human liver grafts reconstruction for transplantation to treat liver disease.

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Section: Introductionmentioning

confidence: 99%

Human-Scale Whole-Organ Bioengineering for Liver Transplantation: A Regenerative Medicine Approach

et al. 2013

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“…xenotransplantation or cell transplantation therapy) have been proposed to solve the problem. Unfortunately, hyperacute rejection [2] and low engraftment efficiency limit their clinical application, respectively. Regenerative medicine may provide novel strategies to treat patients suffering from end-stage organ failure.…”

Section: Introductionmentioning

confidence: 99%

Using a decellularized splenic matrix as a 3D scaffold for hepatocyte cultivation in vitro : a preliminary trial

Zheng

Xiang

et al. 2015

Biomed. Mater.

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Using a decellularized liver matrix (DLM) to reengineer liver tissue is a promising therapy for end-stage liver disease. However, the limited supply of donor organs still hampers its potential clinical application, while a xenogenic decellularized matrix may bring a risk of zoonosis and immunological rejection. Therefore, an appropriate alternative scaffold is needed. In this research, we established a decellularized splenic matrix (DSM) in a rodent model, which preserved the 3D ultrastructure, the components of the extracellular matrix (ECM) and the native vascular network. The DSM and DLM had similar components of ECM, and similar mechanical properties. Hepatocytes were seeded to the DSM and DLM for dynamic culturing up to 6 d, and distributed both in decellularized sinusoidal spaces and around the vessels. The TUNEL-positive cell percentage in a dynamic culturing decellularized splenic matrix (dDSM) was 10.7% ± 3.6% at 3d and 25.8% ± 5.6% at 5d, although 14.2% ± 4.5% and 24.8% ± 2.9%, respectively, in a dynamic culturing decellularized liver matrix (dDLM) at the same time point (p > 0.05). Primary hepatocytes in the dDSM and dDLM expressed albumin, G6pc and Ugt1a1. The gene expression of Cyp2b1, Cyp1a2 and HNF1α in the gene transcription level revealed hepatocytes had lower gene expression levels in the dDSM compared with the dDLM at 3d, but better than those in a sandwich culture. The cumulative albumin production at 6 d of culture was 80.7 ± 9.6 μg per million cells in the dDSM and 89.6 ± 4.6 μg per million cells in the dDLM (p > 0.05). In summary, the DSM is a promising 3D scaffold for hepatocyte cultivation in vitro.

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Xenotransplantation literature update, July–October 2011

Abstract: Schneider MKJ, Seebach JD. Xenotransplantation literature update, July–October 2011. Xenotransplantation 2011; 18: 400–404. © 2011 John Wiley & Sons A/S.

Cited by 2 publications

References 20 publications

Human-Scale Whole-Organ Bioengineering for Liver Transplantation: A Regenerative Medicine Approach

Human-Scale Whole-Organ Bioengineering for Liver Transplantation: A Regenerative Medicine Approach

Using a decellularized splenic matrix as a 3D scaffold for hepatocyte cultivation in vitro : a preliminary trial

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