Xenopus msx-1 regulates dorso-ventral axis formation by suppressing the expression of organizer genes

Takeda, M.; Saito, Yoshinari; Sekine, Ryo; Onitsuka, Izumi; Maeda, Ryo; Maéno, Mitsugu

doi:10.1016/s0305-0491(00)00194-2

Cited by 14 publications

(25 citation statements)

References 43 publications

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“…Two research groups have demonstrated that FGF is a potent neural inducer (Kengaku & Okamoto 1993, 1995Lamb & Harland 1995). Furthermore, in the chicken embryo, it has been shown that FGF can induce posterior neural tissues from epiblasts (Alvarez et al 1998;Storey et al 1998 (Maeda et al 1997;Suzuki et al 1997;Takeda et al 2000. The expression pattern in the gastrula embryo suggested that Xmsx-1 functions in an earlier step of neural tissue formation than that indicated by a previous study (Su et al 1991) …”

Section: It Has Been Widely Accepted Therefore That the Appearanccontrasting

confidence: 47%

“…In the single cap assay, Xmsx-1 inhibited the neurulation induced by noggin or cerberus (Fig. 2), and a dominant-negative form of , which was able to induce the secondary axis if expressed in the prospective ventral regions (Takeda et al 2000), directly induced the expression of N-CAM (Fig. 3) (Onichtchouk et al 1998), in which BMP-4 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it was found that Xmsx-1 is expressed in a broad area, including the presumptive ectoderm (Maeda et al 1997 (Maeda et al 1997;Takeda et al 2000), (Zimmerman et al 1996). Thus, it is assumed that the endogenous expression of Xmsx-1, a direct target gene of the BMP-4 signal, is suppressed by the introduction of a (Xcer, (D) (Fig.…”

Section: Antagonistic Regulation Between Xmsx-1 and Neural-inducing Gmentioning

confidence: 99%

“…The construction of the Xmsx-1/VP-16 fusion gene was described in another paper (Takeda et al 2000). (Amaya et al 1991).…”

Section: Embryos and Rna Injectionmentioning

confidence: 99%

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Involvement of BMP‐4/msx‐1 and FGF pathways in neural induction in the Xenopus embryo

et al. 2000

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The msx homeodomain protein is a downstream transcription factor of the bone morphogenetic protein (BMP)- results of cell-lineage tracing experiments indicated that the neural cells were differentiated from the animal pole tissue where the excess RNA of either BMP-4 or Xmsx-1 was injected, whereas notochord was differentiated from the organizer mesoderm. Neural tissue differentiated from BMP-4-injected ectodermal cells strongly expressed posterior neural markers, such as hoxB9 and krox20, suggesting that the posterior neural cells differentiated regardless of the existence of the BMP signal. The introduction of a dominant-negative form of the fibroblast growth factor (FGF) receptor (XFD) into the ectodermal cells drastically reduced the expression of pan and posterior neural mark

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Section: It Has Been Widely Accepted Therefore That the Appearanccontrasting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Section: Antagonistic Regulation Between Xmsx-1 and Neural-inducing Gmentioning

confidence: 99%

“…The construction of the Xmsx-1/VP-16 fusion gene was described in another paper (Takeda et al 2000). (Amaya et al 1991).…”

Section: Embryos and Rna Injectionmentioning

confidence: 99%

See 2 more Smart Citations

Involvement of BMP‐4/msx‐1 and FGF pathways in neural induction in the Xenopus embryo

et al. 2000

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“…These plasmids were linearized with NotI and RNA was transcribed with Sp6 RNA polymerase. RNA injection was performed according to the method described previously (Takeda et al, 2000). Injected embryos were kept in 50% Steinberg's solution until stages 32-34 for subsequent analyses.…”

Section: Manipulations Of the Embryo And Microinjectionmentioning

confidence: 99%

Neptune is involved in posterior axis and tail formation in Xenopus embryogenesis

Takeda

Kurauchi

Yamazaki

et al. 2005

Developmental Dynamics

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In order to elucidate the molecular mechanisms underlying the posterior axis and tail formation in embryogenesis, the function of Neptune, a zinc-finger transcription factor, in Xenopus laevis embryos was investigated. Injection of neptune mRNA into the animal pole area of embryos resulted in the formation of an additional tail structure that included a neural tube and muscle tissue. This activity required FGF signaling since coinjection of a dominant-negative FGF receptor RNA (XFD) completely blocked the formation of a tail structure. A loss-of-function experiment using a fusion construct of neptune and Drosophila engrailed (en-neptune) RNA showed that endogenous Neptune is necessary for formation of the posterior trunk and tail. Furthermore, activity of Neptune was necessary for the endogenous expression of brachyury and fgf-8 at the late gastrula stage. These findings demonstrate a novel function of Neptune in the process of anterior-posterior axis formation through the FGF and brachyury signaling cascades. An experiment using a combination explant with ventral and dorsal marginal tissues showed that cooperation of these two distinct tissues is important for the tail formation and that expression of Neptune in prospective ventral cells may be involved in the activation of the process of tail formation. Developmental Dynamics 234:63-73, 2005.

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Neural induction and factors that stabilize a neural fate

Rogers

Moody

Casey

2009

Birth Defects Research Pt C

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The neural ectoderm of vertebrates forms when the BMP signaling pathway is suppressed. Herein we review the molecules that directly antagonize extracellular BMP and the signaling pathways that further contribute to reduce BMP activity in the neural ectoderm. Downstream of neural induction, a large number of “neural fate stabilizing” (NFS) transcription factors are expressed in the presumptive neural ectoderm, developing neural tube, and ultimately in neural stem cells. Herein we review what is known about their activities during normal development to maintain a neural fate and regulate neural differentiation. Further elucidation of how the NFS genes interact to regulate neural specification and differentiation should ultimately prove useful for regulating the expansion and differentiation of neural stem and progenitor cells.

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Xenopus msx-1 regulates dorso-ventral axis formation by suppressing the expression of organizer genes

Cited by 14 publications

References 43 publications

Involvement of BMP‐4/msx‐1 and FGF pathways in neural induction in the Xenopus embryo

Involvement of BMP‐4/msx‐1 and FGF pathways in neural induction in the Xenopus embryo

Neptune is involved in posterior axis and tail formation in Xenopus embryogenesis

Neural induction and factors that stabilize a neural fate

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