Xenogeneic and allogeneic anti-MHC immune responses induced by plasmid DNA immunization

Cruz, Charles S. Dela; Chamberlain, John W.; MacDonald, Kelly S.; Barber, Brian H.

doi:10.1016/s0264-410x(99)00082-1

Cited by 9 publications

(2 citation statements)

References 46 publications

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“… 17 , 18 In addition, the ß1 cytokine is also a known chronic inflammatory marker in skin conditions and wound healing models. 2 , 8 , 19 The decrease in TGF-B1 staining in the Cortiva specimens at an average of 7 months postimplantation was surprising. One hypothesis for the decrease in this chronic inflammatory marker is the significantly lower amount of donor DNA found in Cortiva matrix compared with Alloderm (Fig.…”

Section: Discussionmentioning

confidence: 99%

A Histological Comparison of Two Human Acellular Dermal Matrix Products in Prosthetic-Based Breast Reconstruction

Moyer

Hart

Yeager

et al. 2017

Plastic and Reconstructive Surgery - Global Open

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Background:Acellular dermal matrices (ADMs) are an integral component of breast reconstruction. The ideal matrix would be relatively immuno-inert, allow rapid vascularization, and be affordable. The purpose of this study was to histologically compare 2 commonly used ADM products.Methods:This is a prospective histological study of 17 patients (20 breasts) following prosthetic-based breast reconstruction with ADM: Alloderm (LifeCell Corp, Branchburg, N.J.) or Cortiva (RTI Surgical, Alachua Fla.). Biopsies were taken from the dermal matrix and natural capsules surrounding the expander/implant during secondary surgery [Range, 72—694 days (mean, 217 days)]. Biopsy specimens were prepared via hematoxylin and eosin, Masson's trichrome, elastin, and transforming growth factor (TGF)-1 stains. Quantitative analysis of staining was performed with ImageJ software. The clinical outcome of each patient is analyzed in relation to capsule architecture and ADM performance.Results:There were 7 breasts in the Alloderm group and 13 in the Cortiva group. Both groups had similar demographic, aesthetic results, and complication profiles. The TGF-1 staining demonstrated significantly lower levels in the Cortiva capsules (P = 0.0139). The percentage of elastin and collagen are similar in the Cortiva, Alloderm, and natural peri-implant capsules. The native capsules show a significantly greater number of blood vessels when compared with Cortiva and Alloderm (P = 0.0371 and P = 0.0347, respectively); however, there is no difference in vascular pattern between the 2 dermal matrices.Discussion:Postoperatively, Cortiva demonstrates equal vascularity with less TGF-1 activation compared with Alloderm. The clinical success and complication profile were similar between the Alloderm and Cortiva patients.

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Section: Discussionmentioning

confidence: 99%

A Histological Comparison of Two Human Acellular Dermal Matrix Products in Prosthetic-Based Breast Reconstruction

Moyer

Hart

Yeager

et al. 2017

Plastic and Reconstructive Surgery - Global Open

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“…Subsequently, their RBD-HR/trimer protein mixed in MF59 adjuvant was tested as Delta RBD-HR/trimer vaccine in multiple animal models against Delta and Omicron variants and showed protective efficacy. However, the use of human cell-based protein vaccines in animal models can cause artificial xenogeneic protection which has been reported [77][78][79]. Thus, an mRNA or DNA vaccine approach will remove such problems.…”

Section: Discussionmentioning

confidence: 99%

Both Feline Coronavirus Serotypes 1 and 2 Infected Domestic Cats Develop Cross-Reactive Antibodies to SARS-CoV-2 Receptor Binding Domain: Its Implication to Pan-CoV Vaccine Development

Yamamoto

Edison

Rowe-Haas

et al. 2023

Viruses

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The current study was initiated when our specific-pathogen-free laboratory toms developed unexpectedly high levels of cross-reactive antibodies to human SARS-CoV-2 (SCoV2) receptor binding domain (RBD) upon mating with feline coronavirus (FCoV)-positive queens. Multi-sequence alignment analyses of SCoV2 Wuhan RBD and four strains each from FCoV serotypes 1 and 2 (FCoV1 and FCoV2) demonstrated an amino acid sequence identity of 11.5% and a similarity of 31.8% with FCoV1 RBD (12.2% identity and 36.5% similarity for FCoV2 RBD). The sera from toms and queens cross-reacted with SCoV2 RBD and reacted with FCoV1 RBD and FCoV2 spike-2, nucleocapsid, and membrane proteins, but not with FCoV2 RBD. Thus, the queens and toms were infected with FCoV1. Additionally, the plasma from six FCoV2-inoculated cats reacted with FCoV2 and SCoV2 RBDs, but not with FCoV1 RBD. Hence, the sera from both FCoV1-infected cats and FCoV2-infected cats developed cross-reactive antibodies to SCoV2 RBD. Furthermore, eight group-housed laboratory cats had a range of serum cross-reactivity to SCoV2 RBD even 15 months later. Such cross-reactivity was also observed in FCoV1-positive group-housed pet cats. The SCoV2 RBD at a high non-toxic dose and FCoV2 RBD at a 60–400-fold lower dose blocked the in vitro FCoV2 infection, demonstrating their close structural conformations essential as vaccine immunogens. Remarkably, such cross-reactivity was also detected by the peripheral blood mononuclear cells of FCoV1-infected cats. The broad cross-reactivity between human and feline RBDs provides essential insights into developing a pan-CoV vaccine.

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Naked DNA immunization as an approach to target the generic tumor antigen survivin induces humoral and cellular immune responses in mice

et al. 2006

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Xenogeneic and allogeneic anti-MHC immune responses induced by plasmid DNA immunization

Cited by 9 publications

References 46 publications

A Histological Comparison of Two Human Acellular Dermal Matrix Products in Prosthetic-Based Breast Reconstruction

A Histological Comparison of Two Human Acellular Dermal Matrix Products in Prosthetic-Based Breast Reconstruction

Both Feline Coronavirus Serotypes 1 and 2 Infected Domestic Cats Develop Cross-Reactive Antibodies to SARS-CoV-2 Receptor Binding Domain: Its Implication to Pan-CoV Vaccine Development

Naked DNA immunization as an approach to target the generic tumor antigen survivin induces humoral and cellular immune responses in mice

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