Xenodiagnostic Technic as an Aid in Diagnosis of Trichinosis 1

Beck, J. Walter

doi:10.4269/ajtmh.1953.2.97

Cited by 4 publications

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“…Five fishes from the experimentally infected group (Group II) were AN EXPERIMENTAL TRIAL FOR INFECTING CLARIAS 177 LAZERA WITH TRICHINELLA SPIRALIS LARVAE slaughtered on the 1 st , 2 nd , 3 rd , 4 th and 7 th days" post infection, ■Intestinal contents were examined by direct smears for the presence of Z spiralis larvae or adult worms and the whole gut were fed to a group of three non infected albino rats which were then killed on the 40 th day post feeding and examined for the presence of encysted T. spiralis larvae according to Besck (1953). By the end of experiment (40 th day post infection), fishes were slaughtered and parasitologically examined for the presence of encysted muscular T, spiralis larvae through: trichinoscopy (Thornton and Gracey, 1974), combined digestion and Baermann technique (Henriksen, 1973) and feeding of fish muscles to non infected albino rats which were killed on the 40 th day post feeding and examined for the presence of encysted T. spiralis larvae according to Beck (1953).…”

Section: Parasitological Examinationmentioning

confidence: 99%

An Experimental Trial for Infecting the Scavenger Catfish Clarias Lazera With Trichinella Spiralis Larvae With Special Reference to Certain Fish Biochemical Reactions.

Nabih¹,

Rashed²,

Rizkalla³

2003

Egypt. J. of Aquatic Biolo. and Fish.

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S cavenger catfish Glorias gariepius were experimentally infected with viable infective Trichinella spiralis larvae (1000 larvae/fish), Examination of the intestinal contents revealed the presence of viable larvae (that infect albino rats, xenodiagnosis) up to 48 hours post infection. Adult worms were not detected in the intestines of any of the experimentally infected fish. Trichinella spiralis larvae were not detected neither in the muscles of the infected catfish nor in the diaphragms of albino rats fed on muscles obtained from the experimentally infected fish by the 40 th day post infection. Thus, C. gariepius might be considered as a paratenic host for T. spiralis infection and could play a role in the epidemiology of trichinosis. Sequential serum samples from each fish were collected 7 and 40 days post infection. Small but statistically significant changes, in alanine aminotransferase and aspartate aminotransferase occurred after infection. Serum concentration of total protein remained constant, indicating little disturbance of liver function. Infection significantly lowered the relative mobility of serum protein fractions separated by polyacrylamide gel electrophoresis on the 40 th day post infection. The relative intensities of the more mobile fraction (# 1) significantly rose, while fractions # 3, 5 and 11 decreased 40 days post infection. It is concluded that the immune response of C gariepius is probably held responsible for the failure of Trichinella spiralis larvae to establish in this abnormal host.

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Section: Parasitological Examinationmentioning

confidence: 99%