Xenocorneal transplantation

Kim, Mee K; Wee, Won Ryang; Park, Chung Gyu; Kim, Sang J

doi:10.1097/mot.0b013e328344870c

Cited by 41 publications

(34 citation statements)

References 45 publications

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“…As a potential donor species they feature qualities similar in physiology and convenient availability [23], [24]. In some areas of medicine (islet cell transplants, cornea transplants, heart valve replacement, blood transfusions), we already have knowledge of and experience with foreign tissue transplantation [25], [26], [27], [28]. However, serious immunological barriers still prevent whole organ pig-to-human transplantation.…”

Section: Discussionmentioning

confidence: 99%

Morphological Features of the Porcine Lacrimal Gland and Its Compatibility for Human Lacrimal Gland Xenografting

et al. 2013

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In this study, we present first data concerning the anatomical structure, blood supply and location of the lacrimal gland of the pig. Our data indicate that the porcine lacrimal gland may serve as a potential xenograft candidate in humans or as an animal model for engineering of a bioartificial lacrimal gland tissue construct for clinical application. For this purpose, we used different macroscopic preparation techniques and digital reconstruction of the histological gland morphology to gain new insights and important information concerning the feasibility of a lacrimal gland transplantation from pig to humans in general. Our results show that the lacrimal gland of the pig reveals a lot of morphological similarities to the analogous human lacrimal gland and thus might be regarded as a xenograft in the future. This is true for a similar anatomical location within the orbit as well as for the feeding artery supply to the organ. Functional differences concerning the composition of the tear fluid, due to a different secretory unit distribution within the gland tissue will, however, be a challenge in future investigations.

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Section: Discussionmentioning

confidence: 99%

Morphological Features of the Porcine Lacrimal Gland and Its Compatibility for Human Lacrimal Gland Xenografting

et al. 2013

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“…22 The T cell-mediated immune response is known to play a major role in graft rejection. 36,[56][57][58] For example, CD8…”

Section: Discussionmentioning

confidence: 99%

A Subsequent Human Neural Progenitor Transplant into the Degenerate Retina Does Not Compromise Initial Graft Survival or Therapeutic Efficacy

Lü

Lin

Tsai

et al. 2015

Trans. Vis. Sci. Tech.

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“…Porcine corneas are one possibility (1–4). Previously, Pan et al, showed that corneas from Wuzhishan miniature pigs survived for 6 months in rhesus monkeys treated with local corticosteroids (5).…”

Section: Introductionmentioning

confidence: 99%

Development of retrocorneal membrane following pig‐to‐monkey penetrating keratoplasty

Lee

Mammen

Dhaliwal

et al. 2016

Xenotransplantation

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Introduction Recent reports of long-term survival after wild-type (WT) pig-to-monkey corneal xenotransplantation are encouraging. We experienced the rapid development of retrocorneal membranes, a rare complication after corneal allotransplantation (though seen in infants and young children). The original specific aim of the study was to determine the factors associated with successful (young) pig corneal transplantation in monkeys. However, when it was obvious that retrocorneal membranes rapidly developed, our aims became to determine the factors involved in its development after both WT and GE pig corneal xenotransplantation and to investigate the characteristics of the retrocorneal membrane. Methods Rhesus monkeys were recipients of penetrating keratoplasty using WT and GE pigs (n=2, respectively, 1–3 months-old). Local/systemic steroids were administered for 3 months. Grafts were evaluated by slit-lamp for corneal transparency, edema, and neovascularization. Hematoxylin and eosin, Masson trichrome staining, and immunohistochemical analysis were performed. Gal staining was also carried out to distinguish the origin of the membrane. Results All penetrating keratoplasty recipients developed fibrous retrocorneal membranes in the early post-transplantation period, regardless of whether the graft was from a WT or GE pig. There were no features of rejection, with no cell infiltrate in the graft or anterior chamber during the 3 months follow-up. There was no difference in the clinical course between the two groups (WT or GE corneas). Immunohistochemistry indicated that the retrocorneal membranes were CK-negative, α-SMA-positive, and vimentin-positive, suggesting that they were of fibrous (keratocytic) origin. Also, the membrane was Gal positive, suggesting that it is derived from pig cornea. Conclusions Following pig-to-monkey corneal xenotransplantation, we report that retrocorneal membranes are derived from donor pig keratocytes. Prevention of retrocorneal membranes will be necessary to achieve successful corneal xenotransplantation.

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Xenocorneal transplantation

Cited by 41 publications

References 45 publications

Morphological Features of the Porcine Lacrimal Gland and Its Compatibility for Human Lacrimal Gland Xenografting

Morphological Features of the Porcine Lacrimal Gland and Its Compatibility for Human Lacrimal Gland Xenografting

A Subsequent Human Neural Progenitor Transplant into the Degenerate Retina Does Not Compromise Initial Graft Survival or Therapeutic Efficacy

Development of retrocorneal membrane following pig‐to‐monkey penetrating keratoplasty

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