Xenobiotic response in Drosophila melanogaster: Sex dependence of P450 and GST gene induction

Goff, Gaëlle Le; Hilliou, Frédérique; Siegfried, Blair D.; Boundy, Sam; Wajnberg, Éric; Sofer, Luc; Audant, Pascaline; ffrench-Constant, Richard H.; Feyereisen, René

doi:10.1016/j.ibmb.2006.05.009

Cited by 146 publications

(118 citation statements)

References 70 publications

(83 reference statements)

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“…Colony specimens for expression analyses were prepared in Table 1 exactly the same manner as the field-caught test subjects except that they were exposed to control, rather than insecticide-treated papers. To date, sublethal exposure to insecticide is not thought to result in wide-scale gene induction (54)(55)(56). Nevertheless, as there is some evidence for short-lived induction (57), the mosquitoes are held for 24 h postexposure so that any genes that are differentially expressed between groups most likely reflect constitutive rather than induced changes.…”

Section: Methodsmentioning

confidence: 99%

Identification and validation of a gene causing cross-resistance between insecticide classes inAnopheles gambiaefrom Ghana

Mitchell

Stevenson

Müller

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

222

255

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In the last decade there have been marked reductions in malaria incidence in sub-Saharan Africa. Sustaining these reductions will rely upon insecticides to control the mosquito malaria vectors. We report that in the primary African malaria vector, Anopheles gambiae sensu stricto, a single enzyme, CYP6M2, confers resistance to two classes of insecticide. This is unique evidence in a disease vector of cross-resistance associated with a single metabolic gene that simultaneously reduces the efficacy of two of the four classes of insecticide routinely used for malaria control. The gene-expression profile of a highly DDT-resistant population of A. gambiae s.s. from Ghana was characterized using a unique whole-genome microarray. A number of genes were significantly overexpressed compared with two susceptible West African colonies, including genes from metabolic families previously linked to insecticide resistance. One of the most significantly overexpressed probe groups (false-discovery rate-adjusted P < 0.0001) belonged to the cytochrome P450 gene CYP6M2. This gene is associated with pyrethroid resistance in wild A. gambiae s.s. populations) and can metabolize both type I and type II pyrethroids in recombinant protein assays. Using in vitro assays we show that recombinant CYP6M2 is also capable of metabolizing the organochlorine insecticide DDT in the presence of solubilizing factor sodium cholate.

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Section: Methodsmentioning

confidence: 99%

Identification and validation of a gene causing cross-resistance between insecticide classes inAnopheles gambiaefrom Ghana

Mitchell

Stevenson

Müller

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

222

255

View full text Add to dashboard Cite

show abstract

“…Other microarray experiments performed by challenging D. melanogaster to a wide variety of conditions, such as exposure phenobarbital (40)(41)(42)76), ethanol (77,78), starvation (79), and looking at transcriptome changes in response to aging (80) identified a number of P450s that alter expression levels in response to variations in these parameters. We highlight 2 examples in which tissue-expression data can be used to extend microarray results.…”

Section: Temporal Expression Differences Of P450 Between Embryos Andmentioning

confidence: 99%

“…Approaches, such as sex-specific expression of P450s, induction capacity of P450s by various exogenous compounds, and transcriptional profiling have been useful in characterizing P450 functions (39)(40)(41)(42). In an effort to distinguish P450s with essential endogenous functions from those involved in detoxification, we characterized expression patterns of D. melanogaster P450s by in situ hybridization and performed a P450 RNA interference (RNAi) knockdown screen.…”

mentioning

confidence: 99%

Characterization of Drosophila melanogaster cytochrome P450 genes

Chung

Sztal

Pasricha

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

250

232

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Cytochrome P450s form a large and diverse family of hemecontaining proteins capable of carrying out many different enzymatic reactions. In both mammals and plants, some P450s are known to carry out reactions essential for processes such as hormone synthesis, while other P450s are involved in the detoxification of environmental compounds. In general, functions of insect P450s are less well understood. We characterized Drosophila melanogaster P450 expression patterns in embryos and 2 stages of third instar larvae. We identified numerous P450s expressed in the fat body, Malpighian (renal) tubules, and in distinct regions of the midgut, consistent with hypothesized roles in detoxification processes, and other P450s expressed in organs such as the gonads, corpora allata, oenocytes, hindgut, and brain. Combining expression pattern data with an RNA interference lethality screen of individual P450s, we identify candidate P450s essential for developmental processes and distinguish them from P450s with potential functions in detoxification.detoxification genes ͉ in situ hybridization ͉ insecticide resistance ͉ multigene family ͉ RNAi

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“…In D. melanogaster, Cyp12d1 is differentially expressed in response to a diverse array of xenobiotics, including DDT (Festucci-Buselli et al 2005), phenobarbital (Le Goff et al 2006;Sun et al 2006), atrazine (LeGoff et al 2006), caffeine (Willoughby et al 2006), piperonyl butoxide (Willoughby et al 2007), hydrogen peroxide , and pepper (Piper nigrum) extract (Jensen et al 2006), suggesting that it has an important role in the general response to xenobiotics. Field and laboratory selections for DDT resistance in D. melanogaster have resulted in constitutively higher levels of Cyp12d1 transcript in one DDT-resistant strain (Wisconsin) and in higher inducibility in response to DDT in two DDT-resistant strains (91-R and Wisconsin) compared with a susceptible strain (Canton-S) (Pedra et al 2004;Festucci-Buselli et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary Toxicogenomics: Diversification of the Cyp12d1 and Cyp12d3 Genes in Drosophila Species

et al. 2012

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Xenobiotic response in Drosophila melanogaster: Sex dependence of P450 and GST gene induction

Cited by 146 publications

References 70 publications

Identification and validation of a gene causing cross-resistance between insecticide classes inAnopheles gambiaefrom Ghana

Identification and validation of a gene causing cross-resistance between insecticide classes inAnopheles gambiaefrom Ghana

Characterization of Drosophila melanogaster cytochrome P450 genes

Evolutionary Toxicogenomics: Diversification of the Cyp12d1 and Cyp12d3 Genes in Drosophila Species

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