Xao tam phan (Paramignya trimera) methanol extract induced apoptosis in hepatocellular carcinoma HepG2 cell line in vitro

Xao tam phan (XTP), a Paramignya genus of the Citrus family, is a renowned medicinal plant in Southeast Asian countries, including Vietnam, and is associated with treating cancer, hepatitis, diabetes, and nose infections. Extraction is a key operation in the preparation of XTP extracts rich in phytochemical compounds. Recently, the application of ultrasound has resulted in a large number of successes in the extraction of medicinal plants for improvement in yield and quality of extracts. Until present, ultrasonic-assisted extraction has not been favored for XTP. In this work, the effect of sonication treatment on collecting the extract of total phenolics and saponin from the XTP root was investigated, then the experimental data were analyzed and optimized by Response Surface Methodology (RSM). The results indicated that the optimal sonication power, temperature, and time were 200 W, 58°C, and 10 min, respectively. Under those conditions, the total phenolic and saponin contents in the XTP extract were maximized and validated to be 20.70 ± 0.77 mg GAE/g dried matter and 326.30 ± 2.03 mg EE/g dried matter, respectively. For comparison, a higher extraction yield of ultrasound-treated samples, in terms of total phenolics (~ 27%) and total saponin (~ 76%), was found as compared to the untreated ones. In conclusion, the results reveal that the application of ultrasound on xao tam phan root significantly enhanced the phytochemical contents during extraction.

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Influence of Drying Conditions on Physicochemical, Phytochemical, and Antioxidant Properties of Medicinal Plant Stem Xáo Tam Phân (Paramignya trimera) Grown in Vietnam

Nguyen,

Tran,

Nguyen

et al. 2024

CNF

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Introduction: This study aimed to assess the physicochemical, phytochemical, and antioxidant properties of medicinal plant stem Xáo tam phân (Paramignya trimera) as influenced by 11 drying conditions. Xáo tam phân [Paramignya trimera (Oliv.) Guillaum] has been used in fold medicine for cancer prevention and treatment. The preparation of the dried sample is crucial and necessary for further investigation and application. To evaluate the effects of some popular drying methods, namely, sun drying, hot-air drying, microwave drying, infrared drying, and freeze drying on the physicochemical properties, the retention of phytochemical compounds (total saponins, phenolics, and flavonoids), and antioxidant properties (DRSC, CUPRAP, and FRAP) of dried P. trimera stem. Methods: The plant stem Xáo tam phân (Paramignya trimera) was dried by 11 drying conditions: sun drying at 36.6°C; hot-air drying at 60°C, 80°C, and 100°C; microwave drying at 270 W, 450 W, and 720 W; infrared drying at 40°C, 45°C, and 50°C; and freeze drying at -40°C. Results: The results show that among the 11 drying conditions used, hot-air drying at 80C achieved the highest phytochemical contents of the P. trimera stem in terms of total saponins (28.82 mg EE/g DS), total phenolics (3.71 mg GAE/g DS), and total flavonoids (10.82 mg CE/g DS), and infrared drying at 50C possessed the greatest antioxidant activity of the P. trimera stem (3.59 and 9.56 mg TE/g DS for DRSC and CUPRAC, respectively); however, both these drying methods had the longest drying time (1.5 to 7.0 h.) and consumed the highest energy (2.10 to 11.77 kWh). In contrast, microwave drying took the shortest drying time (0.1 to 0.4 h.) and consumed the least energy (0.07 to 0.14 kWh), but it still retained moderate levels of phytochemical compounds and antioxidant activity of the P. trimera stem. Conclusion: The findings achieved from this study recommend using hot-air drying at 80C for the preparation of the dried sample from the P. trimera plant stem.

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Xao tam phan (Paramignya trimera) methanol extract induced apoptosis in hepatocellular carcinoma HepG2 cell line in vitro

Cited by 3 publications

References 12 publications

Phytochemical Composition and Potential Bioactivities of the Essential Oil from the Peels of Paramignya trimera (Oliv.) Guillaum from Vietnam

Phytochemical Composition and Potential Bioactivities of the Essential Oil from the Peels of Paramignya trimera (Oliv.) Guillaum from Vietnam

Ultrasound probe assisted extraction of Paramignya trimera (Oliver) Guillaum root

Influence of Drying Conditions on Physicochemical, Phytochemical, and Antioxidant Properties of Medicinal Plant Stem Xáo Tam Phân (Paramignya trimera) Grown in Vietnam

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