Xanthotoxin reverses Parkinson’s disease-like symptoms in zebrafish larvae and mice models: a comparative study

Abstract

Background The aim of this study is to preliminary evaluate the antiparkinsonian activity of furanocoumarin—xanthotoxin, in two behavioral animal models, zebrafish larvae treated with 6-hydroxydopamine and mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine in order to compare both models. Methods Xanthotoxin was isolated from Pastinaca sativa L. (Apiaceae) fruits. Then, the compound was administered by immersion to zebrafish 5 days after fertilization (dpf) larvae or intraperitoneally to male Swiss mice, as a potential therapeutic agent against locomotor impairments. Results Acute xanthotoxin administration at the concentration of 7.5 µM reversed locomotor activity impairments in 5-dpf zebrafish larvae. In mice model, acute xanthotoxin administration alleviated movement impairments at the concentration of 25 mg/kg. Conclusions The similar activity of the same substance in two different animal models indicates their compatibility and proves the potential of in vivo bioassays based on zebrafish models. Results of our study indicate that xanthotoxin may be considered as a potential lead compound in the discovery of antiparkinsonian drugs.

ErbB2pY‐1248 as a predictive biomarker for Parkinson's disease based on research with RPPA technology and in vivo verification

“…Zebrafish exhibit injured dopaminergic neurons and altered locomotor activity when exposed to MPTP or 6‐OHDA 91,92 . In the current study, zebrafish treated with MPTP or 6‐OHDA induced an apparent decrease in the length of dopaminergic neurons and locomotor activity, which were consistent with the previous research that MPTP or 6‐OHDA could induce PD‐like behavior in a zebrafish model 19,93 . Notably, lapatinib could reverse this decrease to the normal level.…”

Coumarins from Seseli devenyense Simonk.: Isolation by Liquid–Liquid Chromatography and Potential Anxiolytic Activity Using an In Vivo Zebrafish Larvae Model

“…The preliminary HPLC-DAD analysis of the SDMFE revealed the presence of several interesting coumarin compounds. The multi-solvent system HEMWat was previously shown to be efficient in separating compounds from other coumarin-rich sources [ 29 , 30 , 31 , 32 ]. Therefore, various HEMWat mixtures were tested in this work and, from these, HEMWat 2/5/2/5 ( v / v / v / v ) was selected for the separation of devenyol ( 1 ) and cis-khellactone ( 2 ), HEMWat 6/5/6/5 ( v / v / v / v ) for the separation of d-laserpitin ( 3 ) and isolaserpitin ( 4 ) and HEMWat 5/2/5/2 ( v / v / v / v ) for the separation of octanoyllomatin ( 5 ).…”

Xanthotoxin (8‐methoxypsoralen): A review of its chemistry, pharmacology, pharmacokinetics, and toxicity

“…XAT has shown promising results in neurodegenerative diseases (Figure 5), including neurotoxicity, hypoxia‐ischemia‐induced cognitive impairment, AD, and PD (de Oliveira et al, 2014; Koziol, Skalicka‐Wozniak, Michalak, Kaszubska, & Budzynska, 2021).…”

“…Finally, after Knoevenagel reaction and decarboxylation, XAT was obtained (Bandyopadhyay, Datta, & Mal, 2000;Mal, Bandhyopadhyay, Datta, & Murty, 1998). XAT has shown promising results in neurodegenerative diseases (Figure 5), including neurotoxicity, hypoxia-ischemia-induced cognitive impairment, AD, and PD (de Oliveira et al, 2014;Koziol, Skalicka-Wozniak, Michalak, Kaszubska, & Budzynska, 2021).…”