Xanthomonas fragariae

doi:10.1111/j.1365-2338.2006.00926.x

Cited by 25 publications

(3 citation statements)

References 14 publications

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“…Therefore, X. fragariae was listed in 1986 as an A2 quarantine pest on planting stocks within Europe by the European and Mediterranean Plant Protection Organization (EPPO) [18]. X. fragariae causes angular water-soaked spots appearing initially only on the abaxial leaf surface [19]. The size of the lesions increases progressively, which may lead to visible coalescent spots on the upper surface of the leaf [20].…”

Section: Introductionmentioning

confidence: 99%

Host–Pathogen Interactions between Xanthomonas fragariae and Its Host Fragaria × ananassa Investigated with a Dual RNA-Seq Analysis

et al. 2020

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Strawberry is economically important and widely grown, but susceptible to a large variety of phytopathogenic organisms. Among them, Xanthomonas fragariae is a quarantine bacterial pathogen threatening strawberry productions by causing angular leaf spots. Using whole transcriptome sequencing, the gene expression of both plant and bacteria in planta was analyzed at two time points, 12 and 29 days post inoculation, in order to compare the pathogen and host response between the stages of early visible and of well-developed symptoms. Among 28,588 known genes in strawberry and 4046 known genes in X. fragariae expressed at both time points, a total of 361 plant and 144 bacterial genes were significantly differentially expressed, respectively. The identified higher expressed genes in the plants were pathogen-associated molecular pattern receptors and pathogenesis-related thaumatin encoding genes, whereas the more expressed early genes were related to chloroplast metabolism as well as photosynthesis related coding genes. Most X. fragariae genes involved in host interaction, recognition, and pathogenesis were lower expressed at late-phase infection. This study gives a first insight into the interaction of X. fragariae with its host. The strawberry plant changed gene expression in order to consistently adapt its metabolism with the progression of infection.

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Section: Introductionmentioning

confidence: 99%

Host–Pathogen Interactions between Xanthomonas fragariae and Its Host Fragaria × ananassa Investigated with a Dual RNA-Seq Analysis

et al. 2020

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“…allii, X. euvesicatoria, X. hortorum pv. gardneri, X. perforans and X. vesicatoria) still require several steps-observation of disease symptoms, microscopic examination, pathogen isolation, pathogenicity tests and molecular tests [27][28][29][30][31][32][33][34]. Having in mind that only recently X. euroxanthea was proposed as a new species [4], isolated from different plant hosts [7][8][9][10], for which the nature of the bacteria-plant interaction is still unknown, and no distinct symptoms have been described [4], the herein proposed multiplex PCR is the tool available for accurate detection and identification of X. euroxanthea.…”

Section: Discussionmentioning

confidence: 99%

DNA Markers for Detection and Genotyping of Xanthomonas euroxanthea

et al. 2022

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Xanthomonas euroxanthea is a bacterial species encompassing both pathogenic and non-pathogenic strains and is frequently found colonizing the same host plants as X. arboricola. This presents the need to develop a detection and genotyping assay able to track these bacteria in microbial consortia with other xanthomonads. Eight X. euroxanthea-specific DNA markers (XEA1-XEA8) were selected by comparative genomics and validated in silico regarding their specificity and consistency using BLASTn, synteny analysis, CG content, codon usage (CAI/eCAI values) and genomic proximity to plasticity determinants. In silico, the selected eight DNA markers were found to be specific and conserved across the genomes of 11 X. euroxanthea strains, and in particular, five DNA markers (XEA4, XEA5, XEA6, XEA7 and XEA8) were unfailingly found in these genomes. A multiplex of PCR targeting markers XEA1 (819 bp), XEA8 (648 bp) and XEA5 (295 bp) was shown to successfully detect X. euroxanthea down to 1 ng of DNA (per PCR reaction). The topology of trees generated with the concatenated sequences of three markers (XEA5, XEA6 and XEA8) and four housekeeping genes (gyrB, rpoD, fyuA and acnB) underlined the equal discriminatory power of these features and thus the suitability of the DNA markers to discriminate X. euroxanthea lineages. Overall, this study displays a DNA-marker-based method for the detection and genotyping of X. euroxanthea strains, contributing to monitoring for its presence in X. arboricola-colonizing habitats. The present study proposes a workflow for the selection of species-specific detection markers. Prospectively, this assay could contribute to unveil alternative host species of Xanthomonas euroxanthea; and improve the control of phytopathogenic strains.

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“…Xanthomonas fragariae (Kennedy and King 1962a; Kennedy and King 1962b), is the causative agent of angular leaf spot of strawberry. At present, in Europe the pathogen is listed as a quarantine organism on strawberry plants intended for planting (Anonymous 2006). Infections can result in high economic losses as plants should be removed and destroyed upon disease outbreaks (Desmet et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Risks for infection of strawberry plants with an aerosolized inoculum of Xanthomonas fragariae

et al. 2018

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Xanthomonas fragariae is the causative agent of angular leaf spot of strawberry, a quarantine organism in plant propagation material in the European Union. Field experiments were conducted to assess the risks for infection of strawberry plants through dispersal of an aerosolized inoculum. In practice, pathogen aerosols can be formed during mowing of an infected crop or by water splashing on symptomatic plants during overhead irrigation or rain. In our experiments, aerosols were generated by spraying suspensions of X. fragariae with a density of 10 8 cfu ml −1 or water under pressure vertically up into the air. In strawberry plants (cv Elsanta) placed at 1.3, 5 and 10 m distance downwind from the spray boom, infections were found, as evidenced with a combination of dilution-plating and molecular techniques, but more frequently in plants wetted prior to inoculation than in plants kept dry. A logarithmic decrease in infection incidence was found with the distance to the inoculum source. Symptomatic plants were found up to 5 m distance from the inoculum source. No infected plants were found in plants placed 4 m upwind or treated with water. In glasshouse studies, it was shown that under conditions favorable for disease development, spray-inoculation of strawberry plants with estimated densities of X. fragariae as low as 2000 cfu per plant were able to cause symptoms both in cv Elsanta and cv Sonata. Results indicate that there is a considerable risk on infections of strawberry plants exposed to aerosolized inoculum.

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Xanthomonas fragariae

Cited by 25 publications

References 14 publications

Host–Pathogen Interactions between Xanthomonas fragariae and Its Host Fragaria × ananassa Investigated with a Dual RNA-Seq Analysis

Host–Pathogen Interactions between Xanthomonas fragariae and Its Host Fragaria × ananassa Investigated with a Dual RNA-Seq Analysis

DNA Markers for Detection and Genotyping of Xanthomonas euroxanthea

Risks for infection of strawberry plants with an aerosolized inoculum of Xanthomonas fragariae

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