Xanthomonas axonopodis pv. eucalyptorum pv. nov. Causing Bacterial Leaf Blight on Eucalypt in Brazil

Ferraz, Hélvio Gledson Maciel; Badel, Jorge L.; Guimarães, Lúcio Mauro da Silva; Reis, Bruna Paolinelli; Tótola, Marcos Rogério; Gonçalves, Rivadalve C.; Alfenas, and Acelino Couto

doi:10.5423/ppj.oa.01.2018.0014

Cited by 12 publications

(22 citation statements)

References 25 publications

(67 reference statements)

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“…MLSA allowed comparing strains from different species and contributed thus to a better taxonomic framework for the genus Xanthomonas [ 157 ]. Because of its robustness and user-friendliness, MLSA has often been used to identify new pathogens, e.g., pathogens of avocado, eucalypt, lavender, nectarine, peony, radicchio, or roses [ 197 , 198 , 199 , 200 , 201 , 202 , 203 ].…”

Section: Taxonomy and Diversity Of Xanthomonadsmentioning

confidence: 99%

Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

et al. 2021

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Bacteria in the genus Xanthomonas infect a wide range of crops and wild plants, with most species responsible for plant diseases that have a global economic and environmental impact on the seed, plant, and food trade. Infections by Xanthomonas spp. cause a wide variety of non-specific symptoms, making their identification difficult. The coexistence of phylogenetically close strains, but drastically different in their phenotype, poses an added challenge to diagnosis. Data on future climate change scenarios predict an increase in the severity of epidemics and a geographical expansion of pathogens, increasing pressure on plant health services. In this context, the effectiveness of integrated disease management strategies strongly depends on the availability of rapid, sensitive, and specific diagnostic methods. The accumulation of genomic information in recent years has facilitated the identification of new DNA markers, a cornerstone for the development of more sensitive and specific methods. Nevertheless, the challenges that the taxonomic complexity of this genus represents in terms of diagnosis together with the fact that within the same bacterial species, groups of strains may interact with distinct host species demonstrate that there is still a long way to go. In this review, we describe and discuss the current molecular-based methods for the diagnosis and detection of regulated Xanthomonas, taxonomic and diversity studies in Xanthomonas and genomic approaches for molecular diagnosis.

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Section: Taxonomy and Diversity Of Xanthomonadsmentioning

confidence: 99%

Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

et al. 2021

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“…Over 35 species are found in this genus, capable of infecting more than 400 plant hosts (Timilsina et al, 2020). Several species in this genus are causative agents of tree diseases, including X. arboricola pvs juglandis (walnut blight), corylina (hazelnut blight), populi (bark necrosis of poplar), and pruni (bacterial spot of stone fruit and almond); and X. axonopodis pvs citri (Asiatic citrus canker) and eucalyptorum (leaf blight of eucalypt) (Ferraz et al, 2018;Fischer-Le Saux et al, 2015;Garita-Cambronero et al, 2018). Of these pathogens, only three-X.…”

Section: Xanthomonas Sppmentioning

confidence: 99%

“…arboricola pvs juglandis (walnut blight), corylina (hazelnut blight), populi (bark necrosis of poplar), and pruni (bacterial spot of stone fruit and almond); and X . axonopodis pvs citri (Asiatic citrus canker) and eucalyptorum (leaf blight of eucalypt) (Ferraz et al, 2018; Fischer‐Le Saux et al, 2015; Garita‐Cambronero et al, 2018). Of these pathogens, only three— X .…”

Section: Phages For Treatment Of Bacterial Tree Diseasesmentioning

confidence: 99%

Seeing the forest for the trees: Use of phages to treat bacterial tree diseases

et al. 2021

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“…and strains of the Rhizobiaceae family (Gonçalves et al, 2008). More recently, Ferraz et al (2018) recovered bacterial isolates from nursery and field eucalypt plants with BLB symptoms in nine Brazilian states and demonstrated that some of them belong to the proposed pathovar X. axonopodis pv. eucalyptorum based on polyphasic taxonomy, including phylogenetic analysis, fatty acid methyl ester profiling, metabolic fingerprinting and cross-inoculation of diverse plant species.…”

Section: Introductionmentioning

confidence: 99%

Xanthomonas species causing leaf blight on eucalypt plants in Brazil and transfer of Xanthomonas axonopodis pv. eucalyptorum to Xanthomonas citri pv. eucalyptorum comb. nov.

Ferraz,

Badel,

Neves

et al. 2023

Plant Pathology

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Outbreaks of bacterial leaf blight (BLB) frequently affect eucalypt plants under nursery and field conditions in several countries. Although research has been conducted to unveil the causal agent, different bacterial species have been associated with similar disease symptoms in different countries. In order to determine the causal agent of BLB in Brazil, a survey was conducted in nine states to recover bacterial isolates from eucalypt plants exhibiting typical BLB symptoms. A total of 41 yellow‐colony isolates with varying aggressiveness towards a susceptible eucalypt clone were obtained, with 16S rDNA sequences indicating that they belong to the Xanthomonas genus. Rep‐PCR analysis separated the Xanthomonas population affecting eucalypt into six distinct groups revealing its high genetic diversity. The same population formed three clusters together with reference strains of X. citri, X. euvesicatoria and X. phaseoli in a phylogenetic tree constructed with partial dnaK, fyuA, gyrB and rpoD gene sequences. Clustering in the phylogenetic tree was clearly related to grouping based on rep‐PCR. Genome sequence comparisons of representative eucalypt isolates with type strains of validly published Xanthomonas species confirmed that the population consisted of X. citri, X. euvesicatoria and X. phaseoli. Inoculation of tomato, common bean, castor bean and eucalypt plants showed that the representative eucalypt isolates can cause disease in these plant species. Based on the results, the transfer of Xanthomonas axonopodis pv. eucalyptorum to Xanthomonas citri is proposed. These results are relevant for eucalypt BLB management under nursery and field conditions, including selection and deployment of effective plant resistance.

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Xanthomonas axonopodis pv. eucalyptorum pv. nov. Causing Bacterial Leaf Blight on Eucalypt in Brazil

Cited by 12 publications

References 25 publications

Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

Seeing the forest for the trees: Use of phages to treat bacterial tree diseases

Xanthomonas species causing leaf blight on eucalypt plants in Brazil and transfer of Xanthomonas axonopodis pv. eucalyptorum to Xanthomonas citri pv. eucalyptorum comb. nov.

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